LOCUS       V040341                11220 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040341
VERSION     V040341
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4273
                     /label="BTK(NM_000061)"
                     /note="BTK(NM_000061)"
                     /gene="BTK"
     CDS             4280..4345
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4392..4603
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4616..4884
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4913..4922
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4943..6592
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             6662..6715
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6716..7315
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7316..7903
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7977..8210
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8282..8403
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8422..8557
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8595..8611)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8619..8635
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8643..8673)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8688..8709
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8997..9585)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9756..10616)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10617..10721)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11195..11211
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg ccgcagtgat tctggagagc atctttctga agcgatccca
     2341 acagaaaaag aaaacatcac ctctaaactt caagaagcgc ctgtttctct tgaccgtgca
     2401 caaactctcc tactatgagt atgactttga acgtgggaga agaggcagta agaagggttc
     2461 aatagatgtt gagaagatca cttgtgttga aacagtggtt cctgaaaaaa atcctcctcc
     2521 agaaagacag attccgagaa gaggtgaaga gtccagtgaa atggagcaaa tttcaatcat
     2581 tgaaaggttc ccttatccct tccaggttgt atatgatgaa gggcctctct acgtcttctc
     2641 cccaactgaa gaactaagga agcggtggat tcaccagctc aaaaacgtaa tccggtacaa
     2701 cagtgatctg gttcagaaat atcacccttg cttctggatc gatgggcagt atctctgctg
     2761 ctctcagaca gccaaaaatg ctatgggctg ccaaattttg gagaacagga atggaagctt
     2821 aaaacctggg agttctcacc ggaagacaaa aaagcctctt cccccaacgc ctgaggagga
     2881 ccagatcttg aaaaagccac taccgcctga gccagcagca gcaccagtct ccacaagtga
     2941 gctgaaaaag gttgtggccc tttatgatta catgccaatg aatgcaaatg atctacagct
     3001 gcggaagggt gatgaatatt ttatcttgga ggaaagcaac ttaccatggt ggagagcacg
     3061 agataaaaat gggcaggaag gctacattcc tagtaactat gtcactgaag cagaagactc
     3121 catagaaatg tatgagtggt attccaaaca catgactcgg agtcaggctg agcaactgct
     3181 aaagcaagag gggaaagaag gaggtttcat tgtcagagac tccagcaaag ctggcaaata
     3241 tacagtgtct gtgtttgcta aatccacagg ggaccctcaa ggggtgatac gtcattatgt
     3301 tgtgtgttcc acacctcaga gccagtatta cctggctgag aagcaccttt tcagcaccat
     3361 ccctgagctc attaactacc atcagcacaa ctctgcagga ctcatatcca ggctcaaata
     3421 tccagtgtct caacaaaaca agaatgcacc ttccactgca ggcctgggat acggatcatg
     3481 ggaaattgat ccaaaggacc tgaccttctt gaaggagctg gggactggac aatttggggt
     3541 agtgaagtat gggaaatgga gaggccagta cgacgtggcc atcaagatga tcaaagaagg
     3601 ctccatgtct gaagatgaat tcattgaaga agccaaagtc atgatgaatc tttcccatga
     3661 gaagctggtg cagttgtatg gcgtctgcac caagcagcgc cccatcttca tcatcactga
     3721 gtacatggcc aatggctgcc tcctgaacta cctgagggag atgcgccacc gcttccagac
     3781 tcagcagctg ctagagatgt gcaaggatgt ctgtgaagcc atggaatacc tggagtcaaa
     3841 gcagttcctt caccgagacc tggcagctcg aaactgtttg gtaaacgatc aaggagttgt
     3901 taaagtatct gatttcggcc tgtccaggta tgtcctggat gatgaataca caagctcagt
     3961 aggctccaaa tttccagtcc ggtggtcccc accggaagtc ctgatgtata gcaagttcag
     4021 cagcaaatct gacatttggg cttttggggt tttgatgtgg gaaatttact ccctggggaa
     4081 gatgccatat gagagattta ctaacagtga gactgctgaa cacattgccc aaggcctacg
     4141 tctctacagg cctcatctgg cttcagagaa ggtatatacc atcatgtaca gttgctggca
     4201 tgagaaagca gatgagcgtc ccactttcaa aattcttctg agcaatattc tagatgtcat
     4261 ggatgaagaa tccggatccg actacaaaga ccatgacggt gattataaag atcatgacat
     4321 cgactacaag gatgacgatg acaagtagtg agcggccgca aggatctgcg atcgctccgg
     4381 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     4441 cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     4501 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     4561 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc
     4621 tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag
     4681 tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag
     4741 tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact
     4801 cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt
     4861 ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat
     4921 ggagagcgac gagagcggcc tgatggaaga cgccaaaaac ataaagaaag gcccggcgcc
     4981 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata
     5041 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
     5101 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
     5161 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
     5221 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
     5281 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
     5341 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
     5401 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
     5461 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc
     5521 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag
     5581 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
     5641 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
     5701 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
     5761 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc
     5821 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
     5881 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct
     5941 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc
     6001 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
     6061 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag
     6121 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
     6181 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
     6241 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga
     6301 attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc
     6361 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
     6421 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
     6481 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
     6541 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgagatcccg
     6601 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg
     6661 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac
     6721 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     6781 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     6841 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     6901 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     6961 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     7021 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     7081 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     7141 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     7201 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     7261 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca
     7321 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7381 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7441 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7501 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7561 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7621 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7681 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     7741 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7801 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct
     7861 tcgccctcag acgagtcgga tctccctttg gccgcctccc cgcctggtac ctttaagacc
     7921 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7981 agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg
     8041 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8101 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8161 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8221 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8281 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8341 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8401 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg
     8461 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8521 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca
     8581 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8641 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8701 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8761 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8821 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8881 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8941 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     9001 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9061 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9121 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9181 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9241 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9301 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9361 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9421 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9481 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9541 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9601 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9661 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9721 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9781 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9841 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9901 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9961 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    10021 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    10081 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10141 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10201 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10261 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10321 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10381 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10441 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10501 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10561 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10621 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10681 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10741 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    10801 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
    10861 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
    10921 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
    10981 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    11041 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
    11101 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
    11161 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
