LOCUS       V029975                10456 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029975
VERSION     V029975
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2283..3506
                     /label="Ccne1(NM_007633)"
                     /note="Ccne1(NM_007633)"
                     /gene="Ccne1"
     CDS             3513..3578
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        3628..3839
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             3852..4120
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4149..4158
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4179..5828
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             5898..5951
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5952..6551
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6552..7139
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7213..7446
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7518..7639
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7658..7793
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7831..7847)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7855..7871
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7879..7909)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7924..7945
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8233..8821)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8992..9852)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9853..9957)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10431..10447
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag gaattcgcca
     2281 ccatgccaag ggagagagac tcgacggacc acagcaacat gaaagaagaa ggtggctccg
     2341 acctttcagt ccgctccaga aaaaggaagg caaatgtggc cgtgtttttg caagacccag
     2401 atgaagaaat tgccaagatt gacaagactg tgaaaagcga ggatagcagt cagccctggg
     2461 atgataattc agcatgcgtg gacccctgct ctttcatccc cacccctaac aaagaagagg
     2521 acaatgagct tgaatacccc aggactgcat ttcagcctcg gaaaatcaga ccacccagag
     2581 cctccccact tcccgtcttg aattggggca atagagaaga ggtttggagg atcatgttaa
     2641 acaaagaaaa gacttacctg agagatgagc actttctgca gcgtcatcct ctcctgcagg
     2701 cgaggatgag agcagttctt ctggattggc taatggaggt gtgcgaagtc tataagctcc
     2761 acagagagac gttctacttg gcacaggact tctttgatcg ttacatggca tcacaacata
     2821 atatcataaa aacactttta cagcttattg ggatttcagc cttatttatt gcttcaaaac
     2881 ttgaggaaat ctaccctcca aagttgcacc agtttgctta tgttacagat ggcgcttgct
     2941 ccggggatga aattcttacc atggaattga tgatgatgaa ggcccttaag tggcgtctaa
     3001 gccctctgac cattgtgtcc tggctgaatg tctatgtcca agtggcctat gtcaacgaca
     3061 cgggtgaggt gctgatgcct cagtacccac agcaggtctt cgtgcagatc gcagagcttc
     3121 tagacctgtg cgtcctggat gttggctgct tagaatttcc ttatggtgtc ctcgctgctt
     3181 ctgctttgta tcatttctcc tcactggagt tgatgcagaa ggtctcaggt tatcagtggt
     3241 gcgacataga gaagtgtgtc aaatggatgg ttccgttcgc catggttatc cgggagatgg
     3301 gaagttccaa gctcaagcac ttccggggag tccccatgga agactcccac aacatccaga
     3361 cccacaccaa cagcttggat ttgctggaca aagcccaagc aaagaaagcc atattgtcag
     3421 aacagaatag gatttctcct cctccgagtg tggtcctgac acccccaccc agcagtaaga
     3481 agcagagcag cgagcaggag acagaactcg aggactacaa agaccatgac ggtgattata
     3541 aagatcatga catcgactac aaggatgacg atgacaagta aggatccgcg gccgcaagga
     3601 tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     3661 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact
     3721 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     3781 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc
     3841 tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc
     3901 atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt
     3961 ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt
     4021 ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct
     4081 acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta
     4141 cgctagacgc caccatggag agcgacgaga gcggcctgat ggaagacgcc aaaaacataa
     4201 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata
     4261 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
     4321 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga
     4381 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     4441 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     4501 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg
     4561 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa
     4621 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
     4681 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata
     4741 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg
     4801 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg
     4861 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     4921 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     4981 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg
     5041 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     5101 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg
     5161 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag
     5221 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     5281 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag
     5341 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     5401 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     5461 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc
     5521 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag
     5581 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     5641 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     5701 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     5761 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga
     5821 tcgccgtgag atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg
     5881 gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
     5941 atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     6001 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6061 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6121 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6181 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     6241 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     6301 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     6361 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     6421 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     6481 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     6541 ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6601 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6661 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6721 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6781 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6841 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6901 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6961 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7021 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7081 ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc
     7141 tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7201 aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt
     7261 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7321 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7381 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7441 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7501 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7561 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7621 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag
     7681 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7741 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt
     7801 ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7861 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7921 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7981 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8041 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8101 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8161 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8641 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8881 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8941 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9001 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9061 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9121 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9181 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9241 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9301 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9361 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9421 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9481 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9541 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9601 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9661 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9721 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9781 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9841 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9901 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9961 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    10021 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    10081 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    10141 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    10201 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    10261 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    10321 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    10381 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    10441 ggccagtgcc aagctg
//
