LOCUS       V044290                12339 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044290
VERSION     V044290
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5392
                     /label="Cnksr2(NM_177751)"
                     /note="Cnksr2(NM_177751)"
                     /gene="Cnksr2"
     CDS             5399..5464
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5511..5722
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5735..6003
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6032..6041
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6062..7711
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             7781..7834
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7835..8434
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8435..9022
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9096..9329
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9401..9522
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9541..9676
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9714..9730)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9738..9754
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9762..9792)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9807..9828
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10116..10704)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10875..11735)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11736..11840)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12314..12330
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg ctctgataat ggaaccggtg agcaaatggt ctccgagtca
     2341 agtagtggac tggatgaaag gtcttgatga ctgtttgcag cagtacatta agaactttga
     2401 gagagagaag atcagtggag accagctgct acgcattaca catcaggagc tagaagatct
     2461 gggagtcagc cgtattggcc atcaggagct gatcttggaa gcagttgacc ttttgtgtgc
     2521 actgaattat ggcttagaaa cagaaaatct aaaaacactt tctcacaagt tgaatgcatc
     2581 tgccaaaaat ctacaaaact tcataacagg aaggagaagg agcggccatt atgatgggag
     2641 gaccagccga aaattaccaa atgacttttt gacctcagtt gtggatctga ttggagcagc
     2701 caagagtctt cttgcttggt tggacaggtc accatttgct gctgtgacag actattcagt
     2761 cacaagaaat aacgtcatac agctctgctt ggaattaaca acaattgtgc aacaggattg
     2821 cactgtatat gaaactgaaa ataaaattct tcatgtgtgt aaaactctct ctggagtctg
     2881 tgaccacatc atatccctgt catcagatcc tctggtttca cagtctgctc acctggaagt
     2941 gattcagctg gcaaatatca aaccaagtga agggctgggt atgtatatta aatccacata
     3001 tgatggcctc catgtaatta ccgggaccac agaaaattca cctgcagatc gctgtaagaa
     3061 aatccatgct ggtgatgaag taatccaagt gaaccatcag actgtggtgg ggtggcagtt
     3121 gaaaaatttg gtgaatgcac tacgagagga cccgagtggt gttatcttaa ctttgaaaaa
     3181 gcgacctcag agcatgctta cctcagcacc agctttactg aaaaatatga gatggaagcc
     3241 ccttgctctg cagcctctta tacctagaag tcccacaagc agcgttgcca cgccctccag
     3301 caccatcagt acacccacca aaagagacag ttctgctctc caggatctct acatcccccc
     3361 tcctcctgca gaaccatata ttcccaggga tgaaaaagga aaccttcctt gtgaagacct
     3421 cagaggacat atggtgggca agccagttca taaggggtct gaatccccaa attcatttct
     3481 ggaccaagaa tatcgaaaga ggtttaacat tgttgaagaa gatactgtct tatattgcta
     3541 tgaatatgaa aagggacgat caagtagtca aggaagacgg gaaagcacac caacctatgg
     3601 caagctacga cctatatcta tgccagtgga atataattgg gtgggggact atgaagatcc
     3661 aaataagatg aagagagata gtagaagaga aaactctcta cttcgataca tgagcaatga
     3721 aaaaattgct caagaagaat acatgtttca gagaaacagc aaaaaagaca cagggaagaa
     3781 gtcaaaaaag aagggtgata agagtaatag cccagcccac tactcattgc tgcctagttt
     3841 gcaaatggat gcattgagac aagacatcat gggcacacca gtgccagaaa ccacactgta
     3901 ccatacattt caacagtcct ctctgcagca caaatcaaag aagaaaaaca aaggagctat
     3961 atcaggcaag agcaaaagaa gaatttcttg caaggatctt ggccgtggtg actgtgaagg
     4021 atggctttgg aaaaagaaag atgcaaagag ctatttttca cagaaatgga agaagtactg
     4081 gtttgtccta aaggatgcat ccctatactg gtatattaat gaagaggatg aaaaagcaga
     4141 aggattcatc agtctgcctg aatttaaaat cgatagagcc agtgaatgcc gcaagaaata
     4201 tgcattcaaa gcctgccatc ctaaaatcaa aagcttttat tttgctgctg aacatcttga
     4261 tgacatgaac aggtggctta acagaattaa catgctgact gcaggatatg cagaaagaga
     4321 gaggattaaa caagaacaag attactggag cgagagtgac aaagaagaag cagatactcc
     4381 atcgacacca aaacaagaca gccctccgcc cccctatgac acatacccac ggcctccctc
     4441 tatgagttgt gccagtcctt atgtggaagc aaaacacagc cggctttcct ccacagagac
     4501 ctctcagtct cagtcttcac acgaggagtt ccgccaggaa gtcactggga gcagtgctgt
     4561 gtctcccatt cgcaagacag ccagtcagcg ccgctcctgg caggatttaa tcgagacacc
     4621 gctgacaagc tcaggcttac actatcttca gactctgcct ctggaagatt ctgtcttctc
     4681 ggattcagca gccatctccc ctgagcacag gcggcagtcc actcttccaa ctcagaagtg
     4741 ccacctccag gatcattatg gcccataccc tttagcagag agcgagagga tgcaagtgtt
     4801 aaacggcaat gggggcaagc ctcgaagttt tactctgcct cgagatagcg ggttcaacca
     4861 ttgctgtctg aacacaccag ttagtgcctg tgacccacaa gatgacatac agccaccaga
     4921 ggtggaggaa gaagaggagg aggaagaaga ggaagcagca ggagaaaacg ttggagagaa
     4981 aaatgaaaac agggaagaaa aattaggaga ctccttgcaa gatttataca gggcactgga
     5041 ggaagccagt ctgtcaccac taggagaaca tcgcatttca accaagatgg aatacaaact
     5101 atcattcata aaacggtgta atgatcctgt gatgaatgag aaactgcaca ggctgagaat
     5161 tcttaaaagc actttaaagg ccagagaagg ggaagtagcc attattgata aagtcctaga
     5221 caatccagac ttgacttcta aagaattcca acaatggaag cagatgtacc tcgacctttt
     5281 cttggatatc tgtcaaagca ccacctcaaa tgacccacta agtatctctt ctgaagtaga
     5341 tgttctcact tcctctctaa cacacactca ctcatacatt gagacacatg tgggatccga
     5401 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
     5461 caagtagtga gcggccgcaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     5521 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     5581 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     5641 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     5701 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     5761 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     5821 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     5881 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     5941 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     6001 atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct
     6061 gatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg
     6121 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
     6181 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc
     6241 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
     6301 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
     6361 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat
     6421 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca
     6481 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg
     6541 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
     6601 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg
     6661 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc
     6721 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt
     6781 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt
     6841 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta
     6901 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct
     6961 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc
     7021 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata
     7081 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg
     7141 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa
     7201 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg
     7261 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc
     7321 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc
     7381 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca
     7441 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact
     7501 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga
     7561 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga
     7621 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat
     7681 aaaggccaag aagggcggaa agatcgccgt gagatcccgc gctcagtcgt ccaattctgc
     7741 cgtggacggc accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct
     7801 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg
     7861 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     7921 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     7981 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     8041 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     8101 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     8161 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     8221 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     8281 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     8341 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     8401 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg
     8461 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     8521 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     8581 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     8641 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     8701 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     8761 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     8821 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     8881 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     8941 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat
     9001 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     9061 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     9121 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     9181 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9241 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9301 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     9361 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     9421 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9481 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     9541 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9601 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9661 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag
     9721 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9781 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9841 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9901 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    10021 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    10081 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    10141 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    10201 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    10261 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    10321 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10381 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10441 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10501 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    10561 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10621 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10681 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10741 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10801 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    10861 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    10921 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    10981 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    11041 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    11101 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    11161 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    11221 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    11281 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    11341 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    11401 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    11461 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    11521 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    11581 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    11641 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    11701 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    11761 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    11821 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    11881 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    11941 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    12001 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    12061 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    12121 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    12181 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    12241 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    12301 cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
