LOCUS V039307 10087 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039307 VERSION V039307 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1798..1915 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2005..2208 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2283..3137 /label="Miox(NM_019977)" /note="Miox(NM_019977)" /gene="Miox" CDS 3144..3209 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3259..3470 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3483..3751 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 3780..3789 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 3810..5459 /EC_number="" /codon_start=1 /gene="luc+" /note="enhanced luc+ version of the luciferase gene" /product="firefly luciferase" /protein_id="" /transl_table=1 /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" /label="luciferase" CDS 5529..5582 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5583..6182 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6183..6770 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6844..7077 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7149..7270 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7289..7424 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7462..7478) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7486..7502 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7510..7540) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7555..7576 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7864..8452) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8623..9483) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9484..9588) /gene="bla" /label="AmpR promoter" primer_bind 10062..10078 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag gaattcgcca 2281 ccatgaaggt cgatgtgggc ccagaccctt ccctggtcta tcgacccgat gtggacccag 2341 agatggccaa aagcaaggac agcttccgaa actatacttc aggcccgctg ctggatcgtg 2401 tctttaccac atacaagctc atgcacactc accagactgt ggacttcgtc agcaggaagc 2461 gcatccagta tggaagcttc tcttacaaga agatgaccat catggaggct gtgggcatgc 2521 tggatgatct ggtggacgaa tctgacccag acgtagattt ccccaactcc ttccacgcgt 2581 tccagaccgc ggagggcatc cggaaagccc acccggacaa ggactggttc cacctggtcg 2641 gacttttgca cgatctgggg aaaattatgg ctctgtgggg ggaacctcag tgggctgttg 2701 ttggagacac gttccccgtg ggctgccgtc cccaggcctc tgtggtgttc tgtgactcta 2761 ctttccagga caatcctgac ctccaggatc ctcgatacag cacagaactc ggcatgtacc 2821 agcctcactg tggactagag aacgtcctta tgtcctgggg ccatgatgag tacctatacc 2881 agatgatgaa gttcaacaag ttctccctgc cttcagaggc cttctacatg atccgattcc 2941 actccttcta tccgtggcac accggcggtg actaccggca gctgtgcagc cagcaggacc 3001 tggatatgct gccctgggtg caagagttca acaagtttga tctctacacg aagtgccctg 3061 acctaccgga tgtggagagc ctgcggccct actatcaagg gctgattgac aagtactgcc 3121 cgggcaccct gagctggctc gaggactaca aagaccatga cggtgattat aaagatcatg 3181 acatcgacta caaggatgac gatgacaagt aaggatccgc ggccgcaagg atctgcgatc 3241 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg 3301 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg 3361 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag 3421 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc 3481 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc 3541 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct 3601 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac 3661 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg 3721 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagacg 3781 ccaccatgga gagcgacgag agcggcctga tggaagacgc caaaaacata aagaaaggcc 3841 cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga 3901 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca 3961 tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg 4021 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc 4081 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg 4141 aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa 4201 aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta 4261 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc 4321 atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga 4381 caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc 4441 ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa 4501 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta 4561 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag 4621 agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc 4681 tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg 4741 aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt 4801 tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca gctattctga 4861 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag 4921 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt 4981 gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct 5041 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac 5101 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc 5161 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag 5221 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa 5281 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa 5341 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg 5401 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtga 5461 gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg 5521 gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc 5581 ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg 5641 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg 5701 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg 5761 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg 5821 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg 5881 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg 5941 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg 6001 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc 6061 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg 6121 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct 6181 gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 6241 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 6301 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 6361 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 6421 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 6481 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 6541 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 6601 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 6661 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 6721 tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt 6781 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 6841 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt 6901 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6961 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 7021 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 7081 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 7141 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 7201 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 7261 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca 7321 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 7381 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga 7441 cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 7501 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 7561 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 7621 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 7681 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 7741 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 7801 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 7861 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 7921 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 7981 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 8041 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 8101 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 8161 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 8221 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 8281 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 8341 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 8401 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 8461 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 8521 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 8581 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 8641 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 8701 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 8761 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 8821 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 8881 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 8941 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 9001 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 9061 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 9121 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 9181 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 9241 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 9301 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 9361 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 9421 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 9481 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 9541 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 9601 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 9661 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 9721 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 9781 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 9841 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 9901 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 9961 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 10021 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 10081 caagctg //