LOCUS       V028564                10635 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028564
VERSION     V028564
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2285..3763
                     /label="Pltp(NM_011125)"
                     /note="Pltp(NM_011125)"
                     /gene="Pltp"
     promoter        3807..4018
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4031..4299
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4328..4337
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4358..6007
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             6077..6130
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6131..6730
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6731..7318
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7392..7625
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7697..7818
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7837..7972
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8010..8026)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8034..8050
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8058..8088)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8103..8124
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8412..9000)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9171..10031)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10032..10136)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10610..10626
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 caccatggtc ctgctctggg ccctcttcct agcgctcctg gcaggtgccc acgccgagct
     2341 cccgggctgc aagatccgcg tcacctccgc ggctctggat ctggtgaagc aggaaggcct
     2401 gcgctttctg gaacaagagc tggagaccat caccatccca gacgtgtacg gcgcaaaggg
     2461 ccacttttac tacaatatct cggacgtgag ggtcacacag ctgcacctga tatcctcgga
     2521 actccacttc cagccagacc aagatctgct gctgaacatc tccaacgcat ccttggggct
     2581 gcacttccgg aggcagctac tctactggtt cttatatgat gggggctaca tcaatgcctc
     2641 ggcggagggt gtgtccatcc gaacaggtct acagctctcc caggattcca gtggtcggat
     2701 taaagtgtcc aatgtctcct gcgaggcctc tgtgtctaaa atgaatatgg ccttcggggg
     2761 aaccttcagg aggatgtata actttttctc cacgttcatc acttctggga tgcggttcct
     2821 cctcaaccag cagatctgcc ctgtgctcta ccatgctggg acggtgttgc tcaactccct
     2881 cctggacaca gtgcccgtgc gcagttctgt ggatgatctc gtgggcatcg attactccct
     2941 tctgaaggat cctgtggtct ccaacggcaa cctggatatg gaattccggg gagcattctt
     3001 ccctctgaag gaggacaact ggagcctccc caacagggcg gtggagcctc agctggagga
     3061 cgacgagagg atggtgtacg tggccttttc cgagttcttc tttgactctg ccatggagag
     3121 ctacttccag gctggagcgc tgcagctgac acttgttggg gacaaggtgc cgagtgacct
     3181 ggacatgctt ctgagggcca cctactttgg gagcattgtt ctcctgagtc cgacagtgat
     3241 taactcccca ctgaagctga agctagaggc cacgagccct ccacgttgta ccatcaagcc
     3301 gtcaggcacc accatctcca tcaccgccag cgtcaccatc accttggccc cgcccatgtt
     3361 gcccgaagtg gagctgtcca agatgatcat ggaaggccgt ctcagtgcta agttgacact
     3421 ccggggcaag gcgctgcgag tgaagctgga ccttcgaagg tttcaaatct actcaaatca
     3481 gtctgcgctg gagtctctgg cgctgatccc actgcaggcc ccactgaaga cactgctgca
     3541 aatcggagtg atgcctttgc tgaacgagcg tacctggcgt ggggtgcaga tcccccttcc
     3601 tgagggtatc aacttcgtgc gtgaggtggt gaccaaccac gcgggcttcg tcactgttgg
     3661 ggctgacctc cactttgcca aagggcttcg agaagtgatt gacaagaacc gtcctgcaga
     3721 cgttgcggcc tcccatgtcc ccccaccctc tgctgcagct gcctgagcgg ccgcaaggat
     3781 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     3841 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     3901 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     3961 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     4021 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     4081 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     4141 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     4201 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     4261 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     4321 gctagacgcc accatggaga gcgacgagag cggcctgatg gaagacgcca aaaacataaa
     4381 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
     4441 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
     4501 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
     4561 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
     4621 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
     4681 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
     4741 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa
     4801 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
     4861 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag
     4921 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
     4981 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
     5041 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
     5101 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     5161 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
     5221 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     5281 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
     5341 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
     5401 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     5461 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
     5521 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
     5581 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     5641 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
     5701 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
     5761 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     5821 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     5881 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     5941 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
     6001 cgccgtgaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg
     6061 ctccaccgga tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     6121 tcccggccct atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     6181 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     6241 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     6301 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     6361 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     6421 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     6481 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     6541 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     6601 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     6661 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     6721 cggtgcctga aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6781 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6841 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6901 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6961 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7021 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7081 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     7141 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7201 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7261 tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgcct
     7321 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7381 aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg
     7441 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7501 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7561 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7621 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     7681 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7741 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7801 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt
     7861 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7921 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
     7981 tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8041 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8101 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8161 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8221 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8281 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8341 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8401 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8461 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8521 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8581 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8641 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8701 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8761 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8821 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8881 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8941 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9001 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9061 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10141 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
    10201 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
    10261 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    10321 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
    10381 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
    10441 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
    10501 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    10561 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    10621 gccagtgcca agctg
//
