LOCUS       V037653                10493 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037653
VERSION     V037653
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3621
                     /label="Sox4(NM_009238)"
                     /note="Sox4(NM_009238)"
                     /gene="Sox4"
     promoter        3665..3876
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             3889..4157
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4186..4195
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4216..5865
                     /EC_number=""
                     /codon_start=1
                     /gene="luc+"
                     /note="enhanced&nbsp;luc+ version of the luciferase gene"
                     /product="firefly luciferase"
                     /protein_id=""
                     /transl_table=1
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
                     /label="luciferase"
     CDS             5935..5988
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5989..6588
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6589..7176
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7250..7483
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7555..7676
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7695..7830
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7868..7884)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7892..7908
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7916..7946)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7961..7982
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8270..8858)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9029..9889)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9890..9994)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10468..10484
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggtacaa cagaccaaca acgcggagaa cactgaggct
     2341 ctgctggccg gggagagctc ggactcgggc gccggcctgg agctgggcat cgcgtcctcc
     2401 ccgacgcctg gctccaccgc gtcgacgggc ggcaaggcgg acgaccccag ctggtgcaag
     2461 acgcccagtg gccacatcaa gcggcccatg aacgccttta tggtgtggtc gcagatcgag
     2521 cggcgcaaga tcatggagca gtcgcccgac atgcacaacg ccgagatctc caagcggcta
     2581 ggcaaacgct ggaagctgct caaggacagc gacaagattc cgttcatcca ggaggcggag
     2641 cggctgcgcc tcaagcacat ggctgactac cctgactaca agtaccggcc gcgaaagaag
     2701 gtgaagtcgg gcaacgcggg cgcgggatcg gcggccacag ccaagccagg ggagaagggc
     2761 gacaaggtcg cgggcagcag cggccacgcg ggaagcagcc acgcgggggg tggcgcgggc
     2821 ggcagctcca agcccgcgcc caagaagagc tgtggcccca aggtggcggg cagctcggtc
     2881 ggcaagcccc acgctaagct ggtcccggcg ggcggcagca aggcggctgc atcgttctct
     2941 ccagagcaag ctgccctgct gcccctgggg gagcccacgg ccgtctacaa ggtgcggact
     3001 cccagtgcgg ccactccggc cgcctcctcc tcgccgtcca gtgcgctggc caccccagcc
     3061 aaacaccctg ccgacaagaa agtgaagcgc gtctacctgt ttggaagcct gggcgcttcg
     3121 gcgtctcccg tcgggggcct gggagcgagc gccgacccca gtgatccact ggggttgtac
     3181 gaagatggag gcccgggatg ctcgcccgat ggccggagtc tgagcggccg cagcagcgca
     3241 gcatcatcgc cagccgccag ccgatcgccc gctgaccacc gcggctacgc cagcctacgc
     3301 gcagcctcgc ccgccccgtc cagcgcgccc tcgcacgcgt cctcctcgct ctcctcgtcc
     3361 tcttcctcct cctcgggctc ttcgtcgtcc gacgacgagt tcgaagacga cctgctcgac
     3421 ctgaacccca gctcaaactt tgagagcatg tccctgggca gtttcagctc ctcatcggcg
     3481 ctcgatcggg acctggattt taacttcgaa cccggctcag gctcccactt cgaattcccg
     3541 gactattgca cgcccgaggt gagcgagatg atctcgggag attggctgga gtccagcatc
     3601 tctaacctgg tcttcaccta ctgagcggcc gcaaggatct gcgatcgctc cggtgcccgt
     3661 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     3721 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     3781 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     3841 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     3901 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     3961 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     4021 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     4081 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     4141 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     4201 gacgagagcg gcctgatgga agacgccaaa aacataaaga aaggcccggc gccattctat
     4261 ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     4321 gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag
     4381 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     4441 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     4501 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     4561 aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa
     4621 attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa
     4681 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     4741 tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc
     4801 atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc
     4861 tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     4921 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     4981 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg
     5041 agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc
     5101 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt
     5161 ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt
     5221 atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     5281 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     5341 ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct
     5401 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     5461 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt
     5521 gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa
     5581 tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat
     5641 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     5701 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     5761 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     5821 agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgagatc ccgcgctcag
     5881 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     5941 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     6001 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6061 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6121 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6181 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6241 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6301 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6361 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6421 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6481 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6541 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     6601 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6661 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6721 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6781 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6841 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6901 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6961 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     7021 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7081 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct
     7141 cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact
     7201 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7261 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac
     7321 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7381 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7441 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7501 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     7561 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7621 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7681 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7741 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7801 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt
     7861 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7921 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7981 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8041 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8101 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8161 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8221 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8281 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8341 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8401 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8461 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8521 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8581 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8641 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8701 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8761 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8821 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8881 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8941 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9001 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9061 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9121 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9181 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9241 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9301 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9361 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9421 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9481 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9541 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9601 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9661 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9721 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9781 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9841 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9901 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9961 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10021 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    10081 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    10141 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10201 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    10261 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10321 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    10381 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    10441 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
