LOCUS       V034673                10201 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034673
VERSION     V034673
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4348
                     /label="GFPT2(NM_005110)"
                     /note="GFPT2(NM_005110)"
                     /gene="GFPT2"
     promoter        4399..4610
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4623..4891
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4926..5633
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             5643..5696
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5697..6296
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6297..6884
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6958..7191
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7263..7384
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7403..7538
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7576..7592)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7600..7616
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7624..7654)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7669..7690
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7978..8566)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8737..9597)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9598..9702)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10176..10192
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgtgcgg aatctttgcc tacatgaact acagagtccc
     2341 ccggacgagg aaggagatct tcgaaaccct catcaagggc ctgcagcggc tggagtacag
     2401 aggctacgac tcggcaggtg tggcgatcga tgggaataat cacgaagtca aagaaagaca
     2461 cattcagctg gtcaagaaaa gggggaaagt caaggctctc gatgaagaac tttacaaaca
     2521 agacagcatg gacttaaaag tggagtttga gacacacttc ggcattgccc acacgcgctg
     2581 ggccacccac ggggtcccca gtgctgtcaa cagccaccct cagcgctcag acaaaggcaa
     2641 cgaatttgtt gtcatccaca atgggatcat cacaaattac aaagatctga ggaaatttct
     2701 ggaaagcaaa ggctacgagt ttgagtcaga aacagataca gagaccatcg ccaagctgat
     2761 taaatatgtg ttcgacaaca gagaaactga ggacattacg ttttcaacgt tggtcgagag
     2821 agtcattcag cagttggaag gtgcattcgc gctggttttc aagagtgtcc actacccagg
     2881 agaagccgtt gccacacgga gaggcagccc cctgctcatc ggagtccgga gcaaatacaa
     2941 gctctccaca gaacagatcc ctatcttata caggacgtgc actctggaga atgtgaagaa
     3001 tatctgtaag acacggatga agaggctgga cagctccgcc tgcctgcatg ctgtgggcga
     3061 caaggccgtg gaattcttct ttgcttctga tgcaagcgct atcatagagc acaccaaccg
     3121 ggtcatcttc ctggaggacg atgacatcgc cgcagtggct gatgggaaac tctccattca
     3181 ccgggtcaag cgctcggcca gtgatgaccc atctcgagcc atccagacct tgcagatgga
     3241 actgcagcaa atcatgaaag gtaacttcag tgcgtttatg cagaaggaga tcttcgaaca
     3301 gccagaatca gttttcaata ctatgagagg tcgggtgaat tttgaaacca acacagtgct
     3361 cctgggtggc ttgaaggacc acttgaagga gattcgacga tgccgacggc tcatcgtgat
     3421 tggctgtgga accagctacc acgctgccgt ggctacgcgg caagttttgg aggaactgac
     3481 tgagcttcct gtgatggttg aacttgctag tgattttctg gacaggaaca cacctgtgtt
     3541 cagggatgac gtttgctttt tcatcagcca gtcaggcgag accgcggaca ccctcctggc
     3601 gctgcgctac tgtaaggacc gcggcgctct caccgtgggc gtcaccaaca ccgtgggcag
     3661 ctccatctct cgcgagaccg actgcggcgt ccacatcaac gcagggccgg agatcggcgt
     3721 ggccagcacc aaggcttata ccagtcagtt catctctctg gtgatgtttg gtttgatgat
     3781 gtctgaagac cgaatttcac tacaaaacag gaggcaagag atcatccgtg gcttgagatc
     3841 tttacctgag ctgatcaagg aagtgctgtc tctggaggag aagatccacg acttggccct
     3901 ggagctctac acgcagagat cgctgctggt gatggggcgg ggctacaact atgccacctg
     3961 cctggaagga gccctgaaaa ttaaagagat aacctacatg cactcagaag gcatcctggc
     4021 tggggagctg aagcacgggc ccctggcact gattgacaag cagatgcccg tcatcatggt
     4081 cattatgaag gatccttgct tcgccaaatg ccagaacgcc ctgcagcaag tcacggcccg
     4141 ccagggtcgc cccattatac tgtgctccaa ggacgatact gaaagttcca agtttgcgta
     4201 taagacaatt gagctgcccc acactgtgga ctgcctccag ggcatcctga gcgtgattcc
     4261 gctgcagctg ctgtccttcc acctggctgt tctccgagga tatgacgttg acttccccag
     4321 aaatctggcc aagtctgtaa ctgtggaatg aggatccgcg gccgcgaagg atctgcgatc
     4381 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     4441 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     4501 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     4561 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc
     4621 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
     4681 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct
     4741 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac
     4801 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg
     4861 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagacg
     4921 ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     4981 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     5041 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     5101 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     5161 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     5221 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     5281 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     5341 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     5401 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     5461 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     5521 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     5581 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggatctc
     5641 gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga
     5701 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     5761 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     5821 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     5881 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     5941 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6001 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6061 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6121 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6181 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6241 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatc
     6301 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6361 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6421 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6481 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6541 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6601 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6661 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     6721 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6781 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc
     6841 ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta cctttaagac
     6901 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6961 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct
     7021 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7081 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7141 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7201 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7261 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7321 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7381 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc
     7441 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7501 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc
     7561 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7621 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7681 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7741 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7801 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7861 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8401 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8581 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8641 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8701 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     8761 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     8821 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8881 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8941 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9001 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9061 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9121 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9181 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9241 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9301 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9361 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9421 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9481 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9541 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     9601 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9661 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9721 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     9781 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     9841 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     9901 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     9961 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    10021 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
    10081 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
    10141 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
    10201 g
//
