LOCUS       V042468                13030 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042468
VERSION     V042468
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7056
                     /label="GLI3(NM_000168)"
                     /note="GLI3(NM_000168)"
                     /gene="GLI3"
     CDS             7063..7128
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7172..7383
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7396..7664
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7693..7702
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7723..8385
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8455..8508
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8509..9108
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9109..9697
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9771..10004
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10076..10197
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10237..10372
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10405..10421)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10429..10445
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10453..10483)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10498..10519
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10807..11395)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11566..12426)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12427..12531)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13005..13021
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aggcccagtc ccacagctcc
     2341 acgaccactg aaaagaaaaa agttgagaat tccatagtga agtgctccac tcgaacagat
     2401 gtgagcgaga aagccgttgc ctccagcacc acttctaatg aggatgaaag tcctggacag
     2461 acttatcaca gagagagaag aaacgcaatc actatgcagc cacagaatgt ccaggggctc
     2521 agcaaagtca gtgaggaacc ttcaacatcg agtgacgaga gggcctcatt gatcaagaaa
     2581 gagatccatg ggtccctgcc acacgtggcg gagccctctg tgccgtaccg cgggacggtg
     2641 tttgccatgg accccaggaa tggttacatg gagccccact accaccctcc tcatcttttc
     2701 cctgccttcc atcctcctgt accaattgat gccagacatc atgagggccg ttaccattac
     2761 gatccatctc cgattcctcc attgcatatg acttccgcct tatctagtag ccctacgtat
     2821 ccggacctgc ccttcattag gatctcccca caccggaacc ccactgctgc ttccgagtct
     2881 cccttcagcc ctccacatcc ctacattaat ccctacatgg actatatccg ctccttgcac
     2941 agcagcccat cgctctccat gatctcagca acccgtgggc tgagccctac agatgcgccc
     3001 catgcaggag tcagcccagc agaatactat catcagatgg ccctgctaac tggccagcgc
     3061 agcccctatg cagacattat tccctcagct gccaccgccg gcacgggggc catccacatg
     3121 gaatatcttc atgctatgga tagcaccaga ttctccagcc ccaggctgtc agccaggccg
     3181 agccgaaaac gtacactgtc catatcacca ctctccgatc atagctttga ccttcagacc
     3241 atgataagga cgtctcccaa ctccttggtc acgattctca ataattcccg tagcagctct
     3301 tcagcaagtg gctcctatgg tcacttatct gcaagtgcaa tcagccctgc cttgagcttc
     3361 acctactctt ccgcgcccgt ctctctccac atgcatcagc agatcctaag ccgacaacag
     3421 agcttaggtt cagcctttgg acacagccct ccactcatcc accctgcccc aacttttcca
     3481 acacagaggc ctattccagg gatccctacg gttctgaacc ccgtccaggt cagctccggc
     3541 ccttctgagt cctcacagaa caagcccacg agtgagtctg cagtgagcag cactggtgac
     3601 ccgatgcaca acaagaggtc caagatcaaa cccgatgaag acctccccag cccaggggct
     3661 cgggggcagc aggaacagcc cgaaggaaca acccttgtca aggaggaagg ggacaaagat
     3721 gaaagcaaac aggagcctga agtcatctat gagacaaact gccactggga aggctgcgcg
     3781 agggagttcg acacccaaga gcagcttgtg caccatataa ataacgacca tattcatgga
     3841 gagaagaagg agttcgtgtg caggtggctg gactgctcaa gagagcagaa acccttcaaa
     3901 gcccagtata tgttggtagt gcatatgaga agacacacgg gcgagaagcc tcacaaatgc
     3961 acttttgaag gttgcacaaa ggcctactcg agactagaaa acttgaaaac acacttgaga
     4021 tctcacactg gagagaaacc atacgtctgt gagcacgaag gttgcaacaa ggctttctca
     4081 aatgcctctg atcgcgccaa acaccaaaac agaacgcatt ccaatgagaa accatatgtg
     4141 tgcaaaatcc caggctgcac taagcgttac acagacccaa gctccctccg gaaacatgtg
     4201 aagacagtgc atggcccaga ggctcatgtc accaagaagc agcgagggga catccatcct
     4261 cggccgccac ccccgagaga ttccggcagc cattcacagt ccaggtcgcc tggccgaccg
     4321 actcagggag cccttggtga gcagcaggac ctcagcaaca ctacctcaaa gcgggaagaa
     4381 tgcctccagg tgaaaaccgt caaggcagag aagccaatga catctcagcc aagccctggt
     4441 ggtcagtctt catgcagcag ccaacagtcc cccatcagca actattccaa cagtgggctc
     4501 gagcttcctc tgaccgatgg aggtagtata ggagacctca gtgccatcga tgaaacccca
     4561 atcatggact caaccatttc cactgcaacc acagcccttg ctttgcaagc caggagaaac
     4621 ccggcaggga ccaaatggat ggagcacgta aaactagaaa ggctaaaaca agtgaatgga
     4681 atgtttccgc gactgaaccc cattctaccc cctaaagccc ctgcggtctc tcctctcata
     4741 ggaaatggca cacagtccaa caacacctgc agcttgggtg ggcccatgac gcttctcccg
     4801 ggcagaagcg acctctctgg ggtggacgtc actatgctga acatgctcaa cagaagggac
     4861 agcagcgcca gcaccatcag ctcggcctac ctgagcagcc gccgctcctc agggatctcg
     4921 ccctgcttct ccagccgccg ctccagcgag gcgtcacagg ccgagggccg gccgcagaac
     4981 gtgagcgtgg ccgactccta cgaccccatc tccaccgacg cctcgcgccg ctccagcgaa
     5041 gccagccaga gcgacggcct gcccagcctg ctcagcctca cgcccgccca gcagtaccgc
     5101 ctcaaggcca agtacgcggc tgccacagga gggccgccgc cgacgcccct gcccaacatg
     5161 gagaggatga gcctgaagac gcgcctggcg ctgctcgggg atgccctcga gcctggcgtg
     5221 gccctgcctc cagttcatgc cccgaggagg tgcagcgacg ggggagccca cggctacggg
     5281 cggcgccacc tgcagccgca cgatgcgccg ggccacggcg tgaggagggc cagcgacccg
     5341 gtgcggacag gctccgaggg cctggccctg cctcgtgtgc cgcgcttcag cagcctcagc
     5401 agctgcaacc ccccggcgat ggccacgtcc gcggagaagc gcagtctcgt gcttcagaat
     5461 tacacgcggc ccgagggcgg ccagtcccga aacttccact cgtccccctg tcctcccagc
     5521 atcaccgaga acgtcaccct ggagtccctg accatggacg ctgatgccaa cctgaacgat
     5581 gaggatttcc tgccggacga cgtggtgcag tatttaaatt cccagaacca agcagggtac
     5641 gagcagcact tccccagcgc cctcccggac gacagcaaag tgccccacgg gcccggtgac
     5701 tttgacgcgc ccgggctgcc agacagccac gctggccagc agttccatgc cctcgagcag
     5761 ccctgccccg agggcagcaa aaccgacctg cccattcagt ggaacgaagt cagctccgga
     5821 agcgccgacc tgtcctcctc caagctcaag tgtgggccgc ggcccgctgt gccgcagact
     5881 cgcgcctttg ggttctgcaa cggcatggtc gtccacccgc agaacccctt gaggagcggg
     5941 cctgctgggg gctatcagac cctcggggag aacagcaacc cctacggtgg cccagagcac
     6001 ttgatgctcc acaacagccc cggaagtggc accagtggaa acgccttcca tgaacagccc
     6061 tgtaaggccc cgcagtatgg gaactgtctc aacaggcagc cagtggcccc tggtgcactc
     6121 gacggtgcct gtggtgccgg gattcaagcc tcaaagctga agagcacccc catgcaaggg
     6181 agcgggggcc agctgaattt cggcctgccg gtagcgccaa atgagtcagc tggcagcatg
     6241 gtgaatggca tgcagaacca ggacccagtg ggacaggggt acctggctca ccagctcctc
     6301 ggcgacagca tgcagcaccc gggggcaggc cgccccggtc agcagatgct tgggcagatt
     6361 agtgctacct cacacatcaa catctaccaa gggccagaga gctgcctgcc aggggctcac
     6421 ggcatgggca gccagccgtc aagcttggca gttgtcaggg gctaccagcc atgtgccagc
     6481 tttgggggca gcaggcgcca ggctatgccg agggacagcc ttgctctgca gtcaggacag
     6541 ctcagtgaca caagtcagac ctgcagggtg aatggtatca agatggagat gaaagggcag
     6601 ccccatccgc tgtgctctaa tctgcagaat tactctggtc agttctatga ccaaaccgtg
     6661 ggcttcagtc agcaagacac gaaagctggt tcattctcta tttcagacgc cagctgcctg
     6721 ctacagggga ccagcgccaa aaactctgag ttactttccc caggtgctaa tcaggtgaca
     6781 agcacagtgg acagcctcga cagccatgac ctggaagggg tacagattga cttcgatgcc
     6841 atcatagacg atggggacca ctccagcctg atgtcggggg ccctgagccc aagtatcatt
     6901 cagaaccttt cccatagctc ctcccgcctc accacgcctc gggcgtccct cccattccca
     6961 gcgctgtcca tgagcaccac caacatggct atcggggaca tgagttcttt gctgacctcc
     7021 ctagcggaag aaagcaaatt ccttgcagtt atgcaaggat ccgactacaa agaccatgac
     7081 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta gcggccgcga
     7141 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     7201 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     7261 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     7321 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     7381 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     7441 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     7501 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     7561 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     7621 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     7681 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     7741 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     7801 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     7861 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     7921 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     7981 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     8041 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     8101 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     8161 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     8221 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     8281 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     8341 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     8401 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     8461 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     8521 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     8581 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     8641 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     8701 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     8761 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     8821 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     8881 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     8941 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     9001 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     9061 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     9121 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     9181 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     9241 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     9301 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     9361 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     9421 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     9481 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     9541 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     9601 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     9661 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac
     9721 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     9781 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga
     9841 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     9901 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     9961 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
    10021 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
    10081 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    10141 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    10201 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
    10261 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    10321 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
    10381 tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
    10441 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    10501 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    10561 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    10621 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    10681 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    10741 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    10801 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    10861 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    10921 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    10981 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    11041 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    11101 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    11161 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    11221 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
    11281 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    11341 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    11401 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    11461 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    11521 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    11581 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    11641 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    11701 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    11761 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    11821 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    11881 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    11941 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    12001 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    12061 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    12121 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    12181 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    12241 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    12301 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    12361 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    12421 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    12481 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    12541 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    12601 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    12661 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    12721 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
    12781 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    12841 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
    12901 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    12961 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    13021 tgccaagctg
//
