LOCUS       V041698                10877 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041698
VERSION     V041698
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3009
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             3043..3759
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3781..5796
                     /label="GPCPD1(NM_019593)"
                     /note="GPCPD1(NM_019593)"
                     /gene="GPCPD1"
     promoter        5847..6058
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6071..6339
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6366..6965
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6972..7560
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7634..7867
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7939..8060
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8079..8214
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8252..8268)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8276..8292
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8300..8330)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8345..8366
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8654..9242)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9413..10273)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10274..10378)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10852..10868
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggtgagc aagggcgagg aggataacat ggccatcatc
     2341 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     2401 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     2461 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     2521 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     2581 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     2641 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     2701 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     2761 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     2821 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     2881 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     2941 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     3001 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtatggtgag caagggcgag
     3061 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     3121 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     3181 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     3241 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     3301 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     3361 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     3421 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     3481 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     3541 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     3601 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     3661 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     3721 gccgccggga tcactctcgg catggacgag ctgtacaagg aattcggcgg cggagggagt
     3781 atgacacctt ctcaggttgc ctttgaaata agaggaactc ttttaccagg agaagttttt
     3841 gcgatatgtg gaagctgtga tgctttggga aactggaatc ctcaaaatgc tgtggctctt
     3901 cttccagaga atgacacagg tgaaagcatg ctatggaaag caaccattgt actcagtaga
     3961 ggagtatcag ttcagtatcg ctacttcaaa gggtactttt tagaaccaaa gactatcggt
     4021 ggtccatgtc aagtgatagt tcacaagtgg gagactcatc tacaaccacg atcaataacc
     4081 cctttagaaa gcgaaattat tattgacgat ggacaatttg gaatccacaa tggtgttgaa
     4141 actctggatt ctggatggct gacatgtcag actgaaataa gattacgttt gcattattct
     4201 gaaaaacctc ctgtgtcaat aaccaagaaa aaattaaaaa aatctagatt tagggtgaag
     4261 ctgacactag aaggcctgga ggaagatgac gatgataggg tatctcccac tgtactccac
     4321 aaaatgtcca atagcttgga gatatcctta ataagcgaca atgagttcaa gtgcaggcat
     4381 tcacagccgg agtgtggtta tggcttgcag cctgatcgtt ggacagagta cagcatacag
     4441 acgatggaac cagataacct ggaactaatc tttgattttt tcgaagaaga tctcagtgag
     4501 cacgtagttc agggtgatgc ccttcctgga catgtgggta cagcttgtct cttatcatcc
     4561 accattgctg agagtggaaa gagtgctgga attcttactc ttcccatcat gagcagaaat
     4621 tcccggaaaa caataggcaa agtgagagtt gactatataa ttattaagcc attaccagga
     4681 tacagttgtg acatgaaatc ttcattttcc aagtattgga agccaagaat accattggat
     4741 gttggccatc gaggtgcagg aaactctaca acaactgccc agctggctaa agttcaagaa
     4801 aatactattg cttctttaag aaatgctgct agtcatggtg cagcctttgt agaatttgac
     4861 gtacaccttt caaaggactt tgtgcccgtg gtatatcatg atcttacctg ttgtttgact
     4921 atgaaaaaga aatttgatgc tgatccagtt gaattatttg aaattccagt aaaagaatta
     4981 acatttgacc aactccagtt gttaaagctc actcatgtga ctgcactgaa atctaaggat
     5041 cggaaagaat ctgtggttca ggaggaaaat tccttttcag aaaatcagcc atttccttct
     5101 cttaagatgg ttttagagtc tttgccagaa gatgtagggt ttaacattga aataaaatgg
     5161 atctgccagc aaagggatgg aatgtgggat ggtaacttat caacatattt tgacatgaat
     5221 ctgtttttgg atataatttt aaaaactgtt ttagaaaatt ctgggaagag gagaatagtg
     5281 ttttcttcat ttgatgcaga tatttgcaca atggttcggc aaaagcagaa caaatatccg
     5341 atactatttt taactcaagg aaaatctgag atttatcctg aactcatgga cctcagatct
     5401 cggacaaccc ccattgcaat gagctttgca cagtttgaaa atctactggg gataaatgta
     5461 catactgaag acttgctcag aaacccatcc tatattcaag aggcaaaagc taagggacta
     5521 gtcatattct gctggggtga tgataccaat gatcctgaaa acagaaggaa attgaaggaa
     5581 cttggagtta atggtctaat ttatgatagg atatatgatt ggatgcctga acaaccaaat
     5641 atattccaag tggagcaatt ggaacgcctg aagcaggaat tgccagagct taagagctgt
     5701 ttgtgtccca ctgttagccg ctttgttccc tcatctttgt gtggggagtc tgatatccat
     5761 gtggatgcca acggcattga taacgtggag aatgcttagg gatccgcggc cgcgaaggat
     5821 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     5881 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     5941 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     6001 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     6061 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     6121 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     6181 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     6241 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     6301 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     6361 gctagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     6421 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     6481 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     6541 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     6601 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     6661 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     6721 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     6781 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     6841 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     6901 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     6961 cctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7021 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7081 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7141 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7201 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7261 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7321 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7381 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7441 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7501 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7561 ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7621 gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct
     7681 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7741 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7801 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7861 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7921 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7981 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8041 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8101 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8161 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
     8221 tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8281 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8341 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8401 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8461 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8521 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8581 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8641 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8701 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8761 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8821 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8881 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8941 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9001 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9061 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     9121 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9181 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9241 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9301 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9361 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9421 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9481 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9541 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9601 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9661 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9721 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9781 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9841 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9901 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9961 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10021 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10081 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10141 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10201 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10261 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10321 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10381 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
    10441 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
    10501 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    10561 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
    10621 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
    10681 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
    10741 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    10801 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    10861 cggccagtgc caagctg
//
