LOCUS V038714 8110 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038714 VERSION V038714 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..2964 /label="Gpx6(NM_145451)" /note="Gpx6(NM_145451)" /gene="Gpx6" CDS 2971..3036 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3080..3291 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3304..3572 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 3599..4198 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4205..4793 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 4867..5100 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5172..5293 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5312..5447 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(5485..5501) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5509..5525 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5533..5563) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5578..5599 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5887..6475) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6646..7506) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7507..7611) /gene="bla" /label="AmpR promoter" primer_bind 8085..8101 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggcccag aagttgtggg gttcctgtct tttctcattg 2341 tttatggctg ccttagctca ggagactctg aatcctcaaa aatcgaaggt ggattgcaac 2401 aaaggggtga ctggcaccgt ctatgagtat ggagccaaca ccatagatgg tggggagttt 2461 gtcaacttcc agcagtatgc aggaaagcac atcctctttg tcaacgtggc atccttctgt 2521 ggcctgacag ctacgtaccc tgagctgaac acattgcagg aggagctgaa gccattcaac 2581 gtcacggttt tgggctttcc atgcaaccag tttggaaagc aagaacctgg aaagaactca 2641 gagattctcc ttggactcaa gtatgtgcgt ccaggcggtg gctatgtccc caatttccag 2701 ctctttgaga agggggatgt gaacggagac aatgaacaaa aggttttttc tttcctaaag 2761 aactcctgcc ctcccacctc tgaacttttt ggctctccag aacatctctt ctgggatccc 2821 atgaaggttc atgatatccg ctggaacttt gagaagttcc tggtgggacc tgatggagtc 2881 cctgtcatgc gctggttcca ccatactcct gtcagaattg tccagtcaga catcatggag 2941 tacctaaacc aaaccagtac ccagggatcc gactacaaag accatgacgg tgattataaa 3001 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat 3061 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 3121 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 3181 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 3241 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 3301 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 3361 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 3421 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 3481 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 3541 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagat 3601 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg 3661 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg 3721 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat 3781 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag 3841 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc 3901 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc 3961 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag 4021 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga 4081 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga 4141 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt 4201 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt 4261 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc 4321 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga 4381 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 4441 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 4501 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg 4561 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct 4621 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc 4681 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 4741 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac 4801 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 4861 ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg 4921 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 4981 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 5041 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 5101 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 5161 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 5221 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 5281 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc 5341 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 5401 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag 5461 agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 5521 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 5581 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 5641 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 5701 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 5761 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 5821 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 5881 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 5941 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 6001 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 6061 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 6121 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 6181 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 6241 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 6301 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 6361 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 6421 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 6481 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 6541 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 6601 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 6661 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 6721 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 6781 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 6841 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 6901 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 6961 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 7021 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 7081 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 7141 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 7201 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 7261 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 7321 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 7381 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 7441 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 7501 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 7561 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 7621 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 7681 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg 7741 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 7801 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 7861 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 7921 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 7981 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 8041 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 8101 tgccaagctg //