LOCUS V037074 8893 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037074 VERSION V037074 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3747 /label="HDAC1(NM_004964)" /note="HDAC1(NM_004964)" /gene="HDAC1" CDS 3754..3819 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3863..4074 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4087..4355 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4382..4981 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4988..5576 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5650..5883 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5955..6076 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6095..6230 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6268..6284) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6292..6308 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6316..6346) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6361..6382 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6670..7258) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7429..8289) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8290..8394) /gene="bla" /label="AmpR promoter" primer_bind 8868..8884 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggcgcag acgcagggca cccggaggaa agtctgttac 2341 tactacgacg gggatgttgg aaattactat tatggacaag gccacccaat gaagcctcac 2401 cgaatccgca tgactcataa tttgctgctc aactatggtc tctaccgaaa aatggaaatc 2461 tatcgccctc acaaagccaa tgctgaggag atgaccaagt accacagcga tgactacatt 2521 aaattcttgc gctccatccg tccagataac atgtcggagt acagcaagca gatgcagaga 2581 ttcaacgttg gtgaggactg tccagtattc gatggcctgt ttgagttctg tcagttgtct 2641 actggtggtt ctgtggcaag tgctgtgaaa cttaataagc agcagacgga catcgctgtg 2701 aattgggctg ggggcctgca ccatgcaaag aagtccgagg catctggctt ctgttacgtc 2761 aatgatatcg tcttggccat cctggaactg ctaaagtatc accagagggt gctgtacatt 2821 gacattgata ttcaccatgg tgacggcgtg gaagaggcct tctacaccac ggaccgggtc 2881 atgactgtgt cctttcataa gtatggagag tacttcccag gaactgggga cctacgggat 2941 atcggggctg gcaaaggcaa gtattatgct gttaactacc cgctccgaga cgggattgat 3001 gacgagtcct atgaggccat tttcaagccg gtcatgtcca aagtaatgga gatgttccag 3061 cctagtgcgg tggtcttaca gtgtggctca gactccctat ctggggatcg gttaggttgc 3121 ttcaatctaa ctatcaaagg acacgccaag tgtgtggaat ttgtcaagag ctttaacctg 3181 cctatgctga tgctgggagg cggtggttac accattcgta acgttgcccg gtgctggaca 3241 tatgagacag ctgtggccct ggatacggag atccctaatg agcttccata caatgactac 3301 tttgaatact ttggaccaga tttcaagctc cacatcagtc cttccaatat gactaaccag 3361 aacacgaatg agtacctgga gaagatcaaa cagcgactgt ttgagaacct tagaatgctg 3421 ccgcacgcac ctggggtcca aatgcaggcg attcctgagg acgccatccc tgaggagagt 3481 ggcgatgagg acgaagacga ccctgacaag cgcatctcga tctgctcctc tgacaaacga 3541 attgcctgtg aggaagagtt ctccgattct gaagaggagg gagagggggg ccgcaagaac 3601 tcttccaact tcaaaaaagc caagagagtc aaaacagagg atgaaaaaga gaaagaccca 3661 gaggagaaga aagaagtcac cgaagaggag aaaaccaagg aggagaagcc agaagccaaa 3721 ggggtcaagg aggaggtcaa gttggccgga tccgactaca aagaccatga cggtgattat 3781 aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgcg aaggatctgc 3841 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 3901 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa 3961 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 4021 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag 4081 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca 4141 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc 4201 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc 4261 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc 4321 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta 4381 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt 4441 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgacccgga 4501 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 4561 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 4621 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 4681 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 4741 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 4801 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 4861 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 4921 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 4981 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 5041 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 5101 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 5161 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 5221 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 5281 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 5341 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg 5401 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 5461 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 5521 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 5581 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 5641 aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta 5701 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 5761 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 5821 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 5881 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 5941 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 6001 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 6061 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc 6121 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 6181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg 6241 cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 6301 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 6361 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 6421 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 6481 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6541 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 6601 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 6661 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6721 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6781 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6841 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6901 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 6961 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 7021 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 7081 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 7141 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7201 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 7261 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 7321 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 7381 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 7441 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 7501 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 7561 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 7621 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 7681 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 7741 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 7801 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 7861 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 7921 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 7981 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 8041 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 8101 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 8161 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 8221 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 8281 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 8341 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 8401 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 8461 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 8521 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 8581 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 8641 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 8701 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 8761 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 8821 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 8881 cagtgccaag ctg //