LOCUS       V036246                11440 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036246
VERSION     V036246
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5466
                     /label="HERC3(NM_014606)"
                     /note="HERC3(NM_014606)"
                     /gene="HERC3"
     CDS             5473..5538
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5582..5793
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5806..6074
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6103..6112
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6133..6795
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6865..6918
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6919..7518
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7519..8107
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8181..8414
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8486..8607
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8647..8782
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8815..8831)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8839..8855
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8863..8893)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8908..8929
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9217..9805)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9976..10836)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10837..10941)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11415..11431
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt tatgttgggg atattggtct
     2341 ctgggccaac ctggtatcag caccaacctg cagggaattg tggctgagcc ccaggtgtgt
     2401 gggttcatat ctgacagaag tgtcaaggaa gtggcctgtg ggggaaacca ctctgtgttc
     2461 ctgctggaag atggggaagt ttacacatgt ggtttgaaca ccaaggggca actgggccat
     2521 gagagggaag gaaacaagcc agaacaaatt ggagctctgg cagatcagca tatcattcat
     2581 gtggcatgtg gcgagtccca cagtctggcc ctcagtgacc gaggccagct gttttcttgg
     2641 ggtgcaggga gtgatggtca gctaggactc atgactactg aggattctgt ggcagtgccc
     2701 aggttaatac aaaagctgaa ccagcaaaca atattacaag tttcctgtgg caactggcat
     2761 tgcttggctc ttgcggctga tggccagttc ttcacctggg gaaagaacag ccatgggcag
     2821 cttggcttag ggaaggagtt cccctcccaa gccagcccac agagggtgag gtccctggag
     2881 gggatcccac tggctcaggt ggctgccgga ggggctcaca gctttgccct gtctctctca
     2941 ggagctgttt ttggctgggg gatgaataat gccgggcagc tagggctcag tgatgaaaaa
     3001 gatcgagaat ctccatgcca tgtaaaactc ttacgcacgc aaaaagttgt ctatattagt
     3061 tgtggagaag aacacacagc agttctcaca aagagtggag gtgtgtttac ctttggcgct
     3121 ggttcctgtg ggcaacttgg acacgactcc atgaatgatg aggttaaccc tagaagagtt
     3181 ctagagctga tgggtagtga agtaactcaa attgcttgtg gcagacaaca taccctagcc
     3241 ttcgtgcctt cttctggact catctatgca tttggttgtg gagcaagagg tcaattagga
     3301 actgggcaca cttgtaatgt taagtgccca tctcctgtca agggttactg ggctgcccac
     3361 agtggccagc tttcagcccg agctgatcgc tttaaatatc atatcgttaa gcagatcttc
     3421 tctggaggag accagacttt tgtactttgc tccaaatacg agaattattc tcctgctgtt
     3481 gacttcagga ctatgaacca agcacattat accagtttaa taaatgatga aaccatagca
     3541 gtttggagac aaaaactctc agaacacaac aatgcaaata caatcaatgg tgttgttcag
     3601 atattatctt ctgcagcctg ttggaatgga agttttcttg aaaaaaaaat tgatgaacat
     3661 tttaaaacga gtcccaaaat ccctgggatt gacctgaact caactagggt gttatttgag
     3721 aagttaatga actctcagca ctccatgatt ctagaacaga ttttgaacag ttttgaaagt
     3781 tgtctgattc cccagttgtc aagctcacca ccagatgttg aagccatgag aatctattta
     3841 atactacctg agtttcccct actccaggat tccaagtatt atataacatt gactattccc
     3901 ttggctatgg ccattcttcg gctggataca aaccccagca aagtactaga taactggtgg
     3961 tctcaggtat gcccgaaata tttcatgaag ctggtaaacc tctataaagg tgcagtcctt
     4021 tatctactga ggggaagaaa gacattctta attcccgtac tgtttaacaa ttatatcaca
     4081 gcagctctca aactcttgga gaagttatat aaggtaaatc ttaaagtgaa gcatgtggaa
     4141 tatgatacat tttacattcc tgagatttcc aatctcgtgg acattcagga agactacctc
     4201 atgtggttct tgcatcaagc agggatgaag gctagaccat caataataca ggatactgta
     4261 acactttgtt cctacccttt catctttgat gcccaagcca agaccaaaat gttacagaca
     4321 gatgctgaac tacagatgca ggtggcagtc aatggagcca acctgcagaa tgtcttcatg
     4381 cttctcaccc tggagcctct gctggccaga agccccttcc tggtccttca cgttcgcagg
     4441 aacaaccttg ttggagatgc cctaagagag ctgagcattc attctgatat tgatttgaaa
     4501 aagcctctca aagtaatctt tgatggtgaa gaagcagtgg atgccggtgg tgttacaaag
     4561 gaattttttc ttttgctgtt aaaagaactt ttgaatccca tctatggaat gtttacctac
     4621 tatcaagatt caaatctctt gtggttttca gacacgtgtt ttgtagagca caactggttt
     4681 cacttgattg gtataacctg tggactagct atctacaact ccactgtggt cgatctccac
     4741 ttcccattgg ctctctacaa gaagttactc aatgtaaagc ctggcttgga agacttaaag
     4801 gagttgtcac ccactgaagg aaggagtctc caagagcttt tagattaccc cggggaggat
     4861 gtggaggaga ctttctgcct caacttcacg atctgccgag aaagctatgg agtgattgaa
     4921 cagaagaagc tgatacctgg gggagataat gtaactgtgt gcaaggataa caggcaggaa
     4981 tttgtggatg cttatgtgaa ttatgtcttc caaatctcag ttcatgaatg gtacacagcc
     5041 ttctctagtg gcttcctaaa ggtgtgtggt ggcaaagtac ttgagctctt ccagccttca
     5101 gaactgaggg ctatgatggt ggggaacagc aactacaact gggaagaact ggaagagact
     5161 gccatctaca agggagatta ctcggccaca catcccactg taaaactatt ttgggaaaca
     5221 tttcatgagt ttccattgga aaagaagaag aagtttctct tgttcctgac aggcagcgat
     5281 cggattccca tctacggcat ggccagtctg cagattgtca tccagtccac agccagcggg
     5341 gaggagtact tgccggtggc ccacacttgc tacaaccttc ttgacctccc caagtacagc
     5401 agcaaagaga ttctgagtgc ccggctgacc caggcccttg acaactatga agggtttagt
     5461 ttggccggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     5521 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     5581 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5641 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5701 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5761 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     5821 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     5881 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     5941 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     6001 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     6061 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     6121 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     6181 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     6241 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     6301 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     6361 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     6421 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     6481 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     6541 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     6601 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     6661 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     6721 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     6781 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     6841 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     6901 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6961 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7021 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7081 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7141 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7201 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7261 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7321 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7381 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7441 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7501 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7561 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7621 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7681 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7741 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7801 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7861 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7921 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7981 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8041 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8101 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8161 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
     8221 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8281 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8341 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8401 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8461 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     8521 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8581 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     8641 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8701 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8761 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
     8821 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8881 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8941 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9001 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9061 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9121 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9181 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9241 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9301 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9361 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9421 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9481 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9541 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9601 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     9661 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9721 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9781 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9841 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9901 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9961 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10021 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10081 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10141 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10201 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10261 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10321 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10381 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10441 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10501 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10561 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10621 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10681 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10741 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10801 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10861 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10921 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10981 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    11041 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    11101 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    11161 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    11221 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    11281 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    11341 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    11401 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
