LOCUS V041047 10082 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V041047 VERSION V041047 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2317..4083 /label="HNRNPL(NM_001533)" /note="HNRNPL(NM_001533)" /gene="HNRNPL" CDS 4090..4116 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4120..4146 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4150..4176 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" promoter 4241..4452 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4465..4733 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" regulatory 4762..4771 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" CDS 4792..5454 /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" CDS 5524..5577 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5578..6177 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 6178..6765 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6839..7072 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 7144..7265 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 7284..7419 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(7457..7473) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7481..7497 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7505..7535) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7550..7571 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7859..8447) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8618..9478) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9479..9583) /gene="bla" /label="AmpR promoter" primer_bind 10057..10073 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt cgcggaggct gctgccccgg 2341 gcggagaagc ggcgtcggcg gctggagcag aggcagcagc cggacgagca gcggaggcgg 2401 tcgggagcga tggtgaagat ggcggcggcg ggcggcggag gcggcggtgg ccgctactac 2461 ggcggcggca gtgagggcgg ccgggcccct aagcggctca agactgacaa cgccggcgac 2521 cagcacggag gcggcggcgg tggcggtgga ggagccgggg cggcgggcgg cggcggcggt 2581 ggggagaact acgatgaccc gcacaaaacc cctgcctccc cagttgtcca catcaggggc 2641 ctgattgacg gtgtggtgga agcagacctt gtggaggcct tgcaggagtt tggacccatc 2701 agctatgtgg tggtaatgcc taaaaagaga caagcactgg tggagtttga agatgtgttg 2761 ggggcttgca acgcagtgaa ctacgcagcc gacaaccaaa tatacattgc tggtcaccca 2821 gcttttgtca actactctac cagccagaag atctcccgcc ctggggactc ggatgactcc 2881 cggagcgtga acagtgtgct tctctttacc atcctgaacc ccatttattc gatcaccacg 2941 gatgttcttt acactatctg taatccttgt ggccctgtcc agagaattgt cattttcagg 3001 aagaatggag ttcaggcgat ggtggaattt gactcagttc aaagtgccca gcgggccaag 3061 gcctctctca atggggctga tatctattct ggctgttgca ctctgaagat cgaatacgca 3121 aagcctacac gcttgaatgt gttcaagaat gatcaggata cttgggacta cacaaacccc 3181 aatctcagtg gacaaggtga ccctggcagc aaccccaaca aacgccagag gcagccccct 3241 ctcctgggag atcaccccgc agaatatgga gggccccacg gtgggtacca cagccattac 3301 catgatgagg gctacgggcc ccccccacct cactacgaag ggagaaggat gggtccacca 3361 gtggggggtc accgtcgggg cccaagtcgc tacggccccc agtatgggca ccccccaccc 3421 cctcccccac cacccgagta tggccctcac gccgacagcc ctgtgctcat ggtctatggc 3481 ttggatcaat ctaagatgaa ctgtgaccga gtcttcaatg tcttctgctt atatggcaat 3541 gtggagaagg tgaaattcat gaaaagcaag ccgggggccg ccatggtgga gatggctgat 3601 ggctacgctg tagaccgggc cattacccac ctcaacaaca acttcatgtt tgggcagaag 3661 ctgaatgtct gtgtctccaa gcagccagcc atcatgcctg gtcagtcata cgggttggaa 3721 gacgggtctt gcagttacaa agacttcagt gaatcccgga acaatcggtt ctccacccca 3781 gagcaggcag ccaagaaccg catccagcac cccagcaacg tgctgcactt cttcaacgcc 3841 ccgctggagg tgaccgagga gaacttcttt gagatctgcg atgagctggg agtgaagcgg 3901 ccatcttctg tgaaagtatt ctcaggcaaa agtgagcgca gctcctctgg actgctggag 3961 tgggaatcca agagcgatgc cctggagact ctgggcttcc tgaaccatta ccagatgaaa 4021 aacccaaatg gtccataccc ttacactctg aagttgtgtt tctccactgc tcagcacgcc 4081 tccctcgagt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat 4141 tatgctagct atccttatga tgtcccagac tacgcatagg gatccctgcg atatccagcg 4201 cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc 4261 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg 4321 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 4381 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 4441 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct 4501 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc 4561 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt 4621 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg 4681 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg 4741 tgaccggcgc ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg 4801 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 4861 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 4921 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 4981 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 5041 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 5101 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 5161 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 5221 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 5281 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 5341 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 5401 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 5461 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 5521 cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg 5581 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc 5641 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc 5701 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc 5761 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc 5821 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc 5881 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc 5941 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc 6001 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag 6061 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac 6121 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat 6181 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 6241 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 6301 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 6361 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 6421 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 6481 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 6541 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 6601 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 6661 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc 6721 cttcgccctc agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga 6781 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 6841 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc 6901 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 6961 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 7021 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 7081 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 7141 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 7201 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 7261 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 7321 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 7381 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 7441 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 7501 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 7561 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 7621 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 7681 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 7741 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 7801 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 7861 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 7921 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 7981 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 8041 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 8101 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 8161 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 8221 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 8281 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 8341 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 8401 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 8461 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 8521 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 8581 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 8641 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 8701 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 8761 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 8821 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 8881 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 8941 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 9001 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 9061 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 9121 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 9181 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 9241 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 9301 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 9361 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 9421 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 9481 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 9541 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 9601 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 9661 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 9721 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 9781 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 9841 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 9901 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 9961 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 10021 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 10081 tg //