LOCUS V043269 9504 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V043269 VERSION V043269 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1921 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2011..2214 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2303..3586 /label="Hdac3(NM_010411)" /note="Hdac3(NM_010411)" /gene="Hdac3" CDS 3593..3658 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3702..3913 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3926..4194 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4229..4936 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 4946..4999 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS 5000..5599 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5600..6187 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6261..6494 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6566..6687 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6706..6841 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6879..6895) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6903..6919 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6927..6957) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6972..6993 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7281..7869) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8040..8900) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8901..9005) /gene="bla" /label="AmpR promoter" primer_bind 9479..9495 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggccaa gaccgtggcg tatttctacg accccgatgt 2341 gggcaacttc cactatggag ctggacaccc aatgaaacct catcgcctgg cattgactca 2401 tagcctagtc ctgcattatg gtctctataa gaagatgatc gtcttcaagc cttaccaggc 2461 ctcccagcat gacatgtgcc gcttccattc tgaggactac atcgacttcc tgcagagagt 2521 cagccccacc aatatgcagg gtttcaccaa gagccttaat gccttcaacg tgggtgatga 2581 ctgcccagtg tttccaggac ttttcgagtt ctgctcccgt tacacaggcg catctctgca 2641 aggagcaaca cagctaaaca acaagatctg tgatattgcc atcaactggg ccggtggtct 2701 acatcatgcc aagaaatttg aggcctctgg cttctgctat gtcaatgaca tagtaattgg 2761 tatcctggag ctgcttaagt accaccctcg ggtgctctac attgatatcg acatccacca 2821 tggtgacggg gttcaggaag ccttctacct cactgaccgg gtcatgactg tgtccttcca 2881 caaatacgga aattacttct ttcctggaac aggtgacatg tatgaagttg gagcagagag 2941 tggccgctac tattgtctca atgtgccctt acgagatggc attgatgacc agagttacaa 3001 gcaccttttc cagccagtca tcagccaggt ggtggacttc taccagccga cgtgcatcgt 3061 gctccagtgt ggcgctgact ccctgggctg tgatcgatta ggctgcttca atctcagcat 3121 tcgaggacat ggggaatgtg ttgaatatgt caagagtttc aatatccctc tcctggtact 3181 gggaggtggt ggctacactg tccgaaatgt tgcccggtgt tggacatatg aaacatctct 3241 gctggtagaa gaggccatta gtgaggaact tccctatagt gaatacttcg agtactttgc 3301 cccagatttc acactccatc cagatgtcag cacccgcatc gagaatcaga actcacgcca 3361 gtatctggac cagatccgcc agacaatctt tgaaaacttg aagatgctga accatgcacc 3421 cagtgtccag attcatgatg tcccggcaga cctcctgacg tatgacagga ctgacgaggc 3481 cgacgctgaa gagagaggtc ccgaggagaa ctacagcagg ccagaagcac ccaatgagtt 3541 ctatgatggc gaccatgaca acgacaagga aagtgatgtg gagattggat ccgactacaa 3601 agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta 3661 gcggccgcga aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc 3721 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg 3781 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg 3841 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc 3901 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc 3961 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc 4021 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg 4081 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct 4141 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct 4201 gtgaccggcg cctacgctag acgccaccat ggtgagcaag ggcgaggagg ataacatggc 4261 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga 4321 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 4381 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat 4441 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc 4501 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac 4561 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg 4621 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 4681 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa 4741 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc 4801 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga 4861 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat 4921 ggacgagctg tacaagggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt 4981 ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 5041 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 5101 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 5161 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 5221 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 5281 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 5341 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 5401 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 5461 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 5521 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac 5581 ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg 5641 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 5701 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 5761 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 5821 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 5881 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 5941 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 6001 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 6061 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 6121 gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc 6181 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 6241 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct 6301 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6361 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6421 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6481 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6541 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 6601 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 6661 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact 6721 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 6781 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 6841 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga 6901 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 6961 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 7021 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 7081 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 7141 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 7201 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 7261 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 7321 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 7381 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 7441 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 7501 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 7561 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 7621 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 7681 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 7741 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 7801 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 7861 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 7921 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 7981 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 8041 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 8101 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 8161 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 8221 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 8281 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 8341 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 8401 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 8461 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 8521 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 8581 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 8641 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 8701 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 8761 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 8821 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 8881 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 8941 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 9001 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca 9061 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac 9121 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 9181 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg 9241 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 9301 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc 9361 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg 9421 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt 9481 aaaacgacgg ccagtgccaa gctg //