LOCUS       V034784                10967 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034784
VERSION     V034784
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5061
                     /label="Hkdc1(NM_145419)"
                     /note="Hkdc1(NM_145419)"
                     /gene="Hkdc1"
     promoter        5126..5337
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5350..5618
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5647..5656
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5677..6339
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6409..6462
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6463..7062
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7063..7650
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7724..7957
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8029..8150
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8169..8304
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8342..8358)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8366..8382
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8390..8420)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8435..8456
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8744..9332)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9503..10363)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10364..10468)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10942..10958
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgt ttgcagtaca cttggtggcg
     2341 ttttacttca ccaagctgaa ggaggaccag atcaagaagg tggacaggtt cctgtatcac
     2401 atgcggctct cggatgagac cctggtggac atcatggccc gcttccaggc cgagatggag
     2461 aaaggcttgg ggaaggacac caaccccact gcctctgtga agatgctgcc cacattcgtc
     2521 agggccattc cagatggctc agaaaacggg gaattccttt ccctggatct cggaggatcc
     2581 aagtttcggg tcttgaaggt gcaagtctcc caagagggcc agcaaaatgt tcagatggag
     2641 agccagttct acccaatgcc caatgaaatc acccgtggga atggcacgga gctttttgac
     2701 tacgtagccg actgcctggc agatttcatg aagaccaaaa atctgacaca taagaagctg
     2761 cctctgggct tcaccttttc ttttccctgc agacagaata agttggagga gggtgttctg
     2821 ctttcgtgga ccaagaagtt caaggcccgg ggagttcagg acacagacgt ggtgaaccgc
     2881 ctggccactg ccatgaagaa gcacaaggac ctggacgtgg acatcctggc tttggttaac
     2941 gacactgtgg ggaccatgat gacttgtgct tatgatgatc ccaactgtga agtcggcgtt
     3001 atcatcggaa caggcaccaa cgcgtgttac atggaggaca tgagcaacat cgacctcgtg
     3061 gaaggggacg agggaaggat gtgtatcaac acggagtggg gggcctttgg ggacgatggg
     3121 gctttggagg acatccgcac cgagtttgac agagagctgg acctgggctc gctcaacccc
     3181 gggaagcagc tgttcgagaa gatgatcagc gggctgtaca tgggggagct ggtcaggctt
     3241 atcttgctga agatggccaa agttggtctc ctgtttgggg gtgcaaaatc ctctgctctc
     3301 catacaaagg gcaagatcga aacacagcac gtggctgcca tggagatgag taaagaaggc
     3361 ctggccaaca ctcgagagat cctggtggat ttgggcctgg agccctctga gtcagactgc
     3421 atcgctgtcc agcacgtgtg caccatcgtc tcttttcgct cagccaatct ctgcgcagcg
     3481 gctctggcaa ccatcctgac tcgactccga gagaacaaga agctggcccg gctccgcacc
     3541 acagtgggca tggatggaac tctctacaaa acacaccctc agtaccccaa acgcctacac
     3601 aaggtggtga ggagactggt cccaaactgt gatgtccgct tcctcctgtc agagagtgga
     3661 agcaccaagg gggccgccat ggtgaccgca gtggcctctc gggtacaggc ccagcgcaag
     3721 cagatcgaca aggtgctggc tttgttccag ttgacccgag agcaactcct gggtgtgcgg
     3781 gacaagatgc gggctgaact agagtacggg ctgaagaaaa agacccactc tctggctacc
     3841 gtgaagatgc ttcccaccta tgtctatggg atgccggatg gcacagagaa aggaaagttc
     3901 cttgccctgg atttgggggg aaccaacttc cgagtcctcc tggtaaagat caggcggagg
     3961 tctgtgcgca tgtacaacaa gatcttcgcc atccctctgg agatcatgca gggcactggg
     4021 gaggagctct ttgaccacat cgtacagtgc atcgctgact tcctggacta catgggcctc
     4081 aagggcgccc agctgccttt gggcttcacc ttctccttcc cctgcagaca gacatgcatc
     4141 gacaagggga ctctagtagg gtggaccaaa ggcttcaagg ccaccgactg tgaaggggag
     4201 gacgtggtgg acatgctcag agaagccatc aagaggagaa acgagtttga cctggacatt
     4261 gttgcaatcg tgaacgacac agtggggacc atgatgacct gtggctatga agatcccagg
     4321 tgtgagattg gcttgattgc agggacagga agcaacgtgt gctacatgga ggaaatgagg
     4381 aacatcgagc tggtggatgg ggacgaaggc cggatgtgcg tcaacacgga gtggggcggg
     4441 tttggtgaca atggctgcat cgatgacatc cgcacccagt atgacaagga ggtggatgag
     4501 ggctccctga acgccggcaa gcagagatac gagaaaatga ccagtggaat gtacctgggg
     4561 gagattgtga ggcggatcct gatcgacctc acccggcagg ggctcctctt ccgagggcag
     4621 atttcagagc gactccggac caggggcatc tttgaaacca aattcctgtc ccagattgaa
     4681 agtgaccggc tggccctcct gcaggtcagg aggatcctgc agcagctggg cctggacagc
     4741 acgtgcgaag acagtattgt ggtgaaggag gtgtgtgggg ctgtgtctcg gagggccgcc
     4801 cagatgtgtg gtgccggcat ggctgccatc gtggagaaga ggagagaaga ccaaggcctc
     4861 cagcacttca aggtcaccgt gggcgtggat ggcacactgt acaagctgca cccgcacttc
     4921 tcccggatac tgcaggagac ggtgaaagag ctggcccctc agtgtgatgt gacatttatg
     4981 ttgtcagaag acggcagcgg gaagggagca gctctgatca cagctgtagc caagaggtta
     5041 cagcagccac ggaaggacat ctagggatcc ctgcgatatc cagcgcggcc gcgaaggatc
     5101 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     5161 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     5221 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     5281 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     5341 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     5401 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     5461 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     5521 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     5581 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg
     5641 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc
     5701 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc
     5761 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc
     5821 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc
     5881 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag
     5941 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc
     6001 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc
     6061 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg
     6121 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg
     6181 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc
     6241 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg
     6301 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc
     6361 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga
     6421 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc
     6481 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     6541 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6601 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6661 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6721 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6781 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6841 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     6901 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     6961 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     7021 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac
     7081 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7141 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7201 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7261 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     7321 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7381 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7441 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
     7501 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7561 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg
     7621 agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag
     7681 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7741 tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7801 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7861 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7921 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7981 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     8041 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8101 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8161 ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8221 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8281 agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat
     8341 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8401 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8461 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8521 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8581 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8641 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8701 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8761 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8821 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8881 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8941 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9001 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9061 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9121 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9181 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9241 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9301 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9361 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9421 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9481 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9541 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9601 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9661 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9721 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9781 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9841 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     9901 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     9961 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10021 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10081 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10141 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10201 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10261 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10321 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10381 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10441 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    10501 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    10561 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    10621 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    10681 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    10741 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    10801 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    10861 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
    10921 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg
//
