LOCUS       V042504                10715 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042504
VERSION     V042504
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4716
                     /label="Hnrnpu(NM_016805)"
                     /note="Hnrnpu(NM_016805)"
                     /gene="Hnrnpu"
     CDS             4723..4749
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4753..4779
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4783..4809
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4874..5085
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5098..5366
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5395..5404
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5425..6087
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6157..6210
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6211..6810
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6811..7398
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7472..7705
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7777..7898
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7917..8052
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8090..8106)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8114..8130
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8138..8168)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8183..8204
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8492..9080)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9251..10111)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10112..10216)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10690..10706
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga gttcttcgcc tgttaatgtc
     2341 aagaagctga aggtgtcgga gctgaaggag gagctcaaga agcggcgcct ctccgacaag
     2401 ggcctcaagg ccgatctcat ggatcgactc caggccgcgc tggacaacga ggcaggaggc
     2461 cgccccgcca tggagcccgg gaacggcagt ctcgacctag gtggcgatgc ggccgggcgc
     2521 tcgggagcgg gcctagagca ggaggccgcg gctggcgccg aagacgacga ggaggaggag
     2581 ggcatcgccg ctctggacgg cgaccagatg gagctgggcg aggagaacgg ggcggcgggg
     2641 gcggctgacg ccggcgcgat ggaggaagag gaggcagcgt cggaagacga gaacggcgac
     2701 gaccagggct tccaggaggg ggaagacgag ctcggcgacg aggaggaggg cgcgggcgac
     2761 gagaacggtc acggggagca gcagtcccaa ccgccggcag cggcggcgca gcagcagcct
     2821 tcccagcagc gtggtgccgg caaggaggcc gcgggcaaga gcagcggccc cacctcgctc
     2881 ttcgcggtga cggtggcgcc gccaggggcg aggcagggcc aacagcaggc gggaggagac
     2941 ggcaaaacag aacagaaagg tggagataaa aagagaggcg ttaaaagacc gcgagaagat
     3001 catggccgag ggtattttga gtacatcgaa gaaaacaagt acagcagagc caagtctcct
     3061 cagccacctg ttgaagaaga agacgaacac ttcgatgaca cagtggtttg tcttgatact
     3121 tataattgtg atctgcattt taaaatctcg agagaccgtc tgagtgcttc ttcccttact
     3181 atggagagtt ttgctttcct gtgggctgga ggaagagctt cctacggtgt gtcaaaaggc
     3241 aaagtctgct ttgagatgaa ggtaacagag aagattccag taagacactt atatacaaaa
     3301 gatattgata tacatgaagt tcggattggc tggtcactaa ccacaagtgg aatgttgctt
     3361 ggtgaagaag aattttctta cgggtattct ctgaaaggaa taaaaacatg caactgtgag
     3421 acagaagatt atggggagaa gtttgatgaa aatgatgtga ttacatgctt tgctaacttt
     3481 gaaactgatg aagttgaact ctcttatgcg aagaatggac aagatcttgg tgttgccttt
     3541 aagatcagta aggaagttct tgctgaccgg ccactattcc cacatgttct ctgccataac
     3601 tgtgcagttg aatttaattt cggtcaaaag gaaaagccat actttccaat acctgaagac
     3661 tgtactttta tccaaaatgt ccccttagag gaccgagtta gaggaccaaa aggacctgaa
     3721 gagaagaagg attgtgaggt tgtaatgatg attggcttgc caggagctgg aaaaactacc
     3781 tgggttacta aacatgcagc tgaaaaccct gggaaataca acattcttgg aacaaatacg
     3841 attatggaca agatgatggt ggcaggtttt aagaagcaaa tggcagatac tggaaaactg
     3901 aacacactgt tgcagagagc cccacagtgt cttggcaagt ttattgaaat tgctgcccgt
     3961 aagaagcgaa attttattct ggatcagaca aatgtgtctg ctgctgccca gagaagaaaa
     4021 atgtgcctgt ttgcaggctt ccagcggaaa gctgttgtag tgtgcccaaa agatgaagac
     4081 tataagcaga ggacacagaa gaaggcagaa gtagagggga aggacctacc agaacatgct
     4141 gtcctcaaga tgaaaggaaa cttcaccctt ccagaggttg cagaatgctt tgatgaaata
     4201 acctatgttg aacttcagaa agaggaagcc caaaagcttt tggagcaata taaagaagaa
     4261 agcaaaaagg cactgccacc agaaaagaag caaaacactg gctcaaagaa aagcaataag
     4321 aataagagtg gcaagaacca gttcaacaga ggtggtggcc atagaggccg tggaggattc
     4381 aatatgcgag gtggcaattt cagaggagga gctcctggga atcgtggtgg atataatagg
     4441 agaggcaaca tgccacagag aggtggtggc ggtggaagtg gtggaattgg ctatccatac
     4501 ccacgtggcc ctgtttttcc tggccgaggt ggttactcaa acagagggaa ttacaacaga
     4561 ggtggaatgc ccaacagagg gaactataac cagaacttca gaggacgagg aaataatcgt
     4621 ggctacaaaa atcaatctca gggctacaat cagtggcagc agggtcaatt ctggggtcag
     4681 aagccatgga gtcagcatta tcaccaagga tattatctcg agtacccata cgacgtacct
     4741 gattatgcaa cttatcctta tgatgtacct gattatgcta gctatcctta tgatgtccca
     4801 gactacgcat agggatccct gcgatatcca gcgcggccgc gaaggatctg cgatcgctcc
     4861 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     4921 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     4981 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     5041 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     5101 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5161 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5221 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5281 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5341 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     5401 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     5461 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     5521 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     5581 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     5641 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     5701 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     5761 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     5821 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     5881 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     5941 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     6001 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     6061 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     6121 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     6181 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     6241 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6301 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6361 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6421 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6481 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6541 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6601 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6661 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6721 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6781 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
     6841 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6901 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6961 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7021 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7081 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7141 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7201 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7261 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7321 ctgccggctc tgcggcctct tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc
     7381 ctttggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat
     7441 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaaa
     7501 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7561 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7621 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7681 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7741 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     7801 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7861 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     7921 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7981 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8041 tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg tcatagctgt
     8101 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8161 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8221 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8281 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8341 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8401 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8461 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8521 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8581 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8641 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8701 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8761 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8821 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8881 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     8941 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9001 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9061 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9121 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9181 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9241 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9301 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9361 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9421 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9481 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9541 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9601 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9661 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9721 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9781 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9841 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9901 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9961 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10021 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10081 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10141 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10201 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    10261 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
    10321 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    10381 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    10441 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    10501 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
    10561 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    10621 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    10681 cacgacgttg taaaacgacg gccagtgcca agctg
//
