LOCUS       V042327                15200 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042327
VERSION     V042327
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..9628
                     /label="CREBBP(NM_004380)"
                     /note="CREBBP(NM_004380)"
                     /gene="CREBBP"
     CDS             9635..9700
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        9743..9954
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             9967..10235
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             10263..11288
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /note="confers resistance to hygromycin"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E*"
                     /label="HygR"
     misc_feature    11295..11883
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             11957..12190
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    12262..12383
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      12402..12537
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(12575..12591)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    12599..12615
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(12623..12653)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    12668..12689
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(12977..13565)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(13736..14596)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(14597..14701)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     15175..15191
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcc ggatccgcca ccatggctga gaacttgctg gacggaccgc ccaaccccaa
     2341 aagagccaaa ctcagctcgc ccggtttctc ggcgaatgac agcacagatt ttggatcatt
     2401 gtttgacttg gaaaatgatc ttcctgatga gctgataccc aatggaggag aattaggcct
     2461 tttaaacagt gggaaccttg ttccagatgc tgcttccaaa cataaacaac tgtcggagct
     2521 tctacgagga ggcagcggct ctagtatcaa cccaggaata ggaaatgtga gcgccagcag
     2581 ccccgtgcag cagggcctgg gtggccaggc tcaagggcag ccgaacagtg ctaacatggc
     2641 cagcctcagt gccatgggca agagccctct gagccaggga gattcttcag cccccagcct
     2701 gcctaaacag gcagccagca cctctgggcc cacccccgct gcctcccaag cactgaatcc
     2761 gcaagcacaa aagcaagtgg ggctggcgac tagcagccct gccacgtcac agactggacc
     2821 tggtatctgc atgaatgcta actttaacca gacccaccca ggcctcctca atagtaactc
     2881 tggccatagc ttaattaatc aggcttcaca agggcaggcg caagtcatga atggatctct
     2941 tggggctgct ggcagaggaa ggggagctgg aatgccgtac cctactccag ccatgcaggg
     3001 cgcctcgagc agcgtgctgg ctgagaccct aacgcaggtt tccccgcaaa tgactggtca
     3061 cgcgggactg aacaccgcac aggcaggagg catggccaag atgggaataa ctgggaacac
     3121 aagtccattt ggacagccct ttagtcaagc tggagggcag ccaatgggag ccactggagt
     3181 gaacccccag ttagccagca aacagagcat ggtcaacagt ttgcccacct tccctacaga
     3241 tatcaagaat acttcagtca ccaacgtgcc aaatatgtct cagatgcaaa catcagtggg
     3301 aattgtaccc acacaagcaa ttgcaacagg ccccactgca gatcctgaaa aacgcaaact
     3361 gatacagcag cagctggttc tactgcttca tgctcataag tgtcagagac gagagcaagc
     3421 aaacggagag gttcgggcct gctcgctccc gcattgtcga accatgaaaa acgttttgaa
     3481 tcacatgacg cattgtcagg ctgggaaagc ctgccaagtt gcccattgtg catcttcacg
     3541 acaaatcatc tctcattgga agaactgcac acgacatgac tgtcctgttt gcctcccttt
     3601 gaaaaatgcc agtgacaagc gaaaccaaca aaccatcctg gggtctccag ctagtggaat
     3661 tcaaaacaca attggttctg ttggcacagg gcaacagaat gccacttctt taagtaaccc
     3721 aaatcccata gaccccagct ccatgcagcg agcctatgct gctctcggac tcccctacat
     3781 gaaccagccc cagacgcagc tgcagcctca ggttcctggc cagcaaccag cacagcctca
     3841 aacccaccag cagatgagga ctctcaaccc cctgggaaat aatccaatga acattccagc
     3901 aggaggaata acaacagatc agcagccccc aaacttgatt tcagaatcag ctcttccgac
     3961 ttccctgggg gccacaaacc cactgatgaa cgatggctcc aactctggta acattggaac
     4021 cctcagcact ataccaacag cagctcctcc ttctagcacc ggtgtaagga aaggctggca
     4081 cgaacatgtc actcaggacc tgcggagcca tctagtgcat aaactcgtcc aagccatctt
     4141 cccaacacct gatcccgcag ctctaaagga tcgccgcatg gaaaacctgg tagcctatgc
     4201 taagaaagtg gaaggggaca tgtacgagtc tgccaacagc agggatgaat attatcactt
     4261 attagcagag aaaatctaca agatacaaaa agaactagaa gaaaaacgga ggtcgcgttt
     4321 acataaacaa ggcatcttgg ggaaccagcc agccttacca gccccggggg ctcagccccc
     4381 tgtgattcca caggcacaac ctgtgagacc tccaaatgga cccctgtccc tgccagtgaa
     4441 tcgcatgcaa gtttctcaag ggatgaattc atttaacccc atgtccttgg ggaacgtcca
     4501 gttgccacaa gcacccatgg gacctcgtgc agcctcccca atgaaccact ctgtccagat
     4561 gaacagcatg ggctcagtgc cagggatggc catttctcct tcccgaatgc ctcagcctcc
     4621 gaacatgatg ggtgcacaca ccaacaacat gatggcccag gcgcccgctc agagccagtt
     4681 tctgccacag aaccagttcc cgtcatccag cggggcgatg agtgtgggca tggggcagcc
     4741 gccagcccaa acaggcgtgt cacagggaca ggtgcctggt gctgctcttc ctaaccctct
     4801 caacatgctg gggcctcagg ccagccagct accttgccct ccagtgacac agtcaccact
     4861 gcacccaaca ccgcctcctg cttccacggc tgctggcatg ccatctctcc agcacacgac
     4921 accacctggg atgactcctc cccagccagc agctcccact cagccatcaa ctcctgtgtc
     4981 gtcttccggg cagactccca ccccgactcc tggctcagtg cccagtgcta cccaaaccca
     5041 gagcacccct acagtccagg cagcagccca ggcccaggtg accccgcagc ctcaaacccc
     5101 agttcagccc ccgtctgtgg ctacccctca gtcatcgcag caacagccga cgcctgtgca
     5161 cgcccagcct cctggcacac cgctttccca ggcagcagcc agcattgata acagagtccc
     5221 taccccctcc tcggtggcca gcgcagaaac caattcccag cagccaggac ctgacgtacc
     5281 tgtgctggaa atgaagacgg agacccaagc agaggacact gagcccgatc ctggtgaatc
     5341 caaaggggag cccaggtctg agatgatgga ggaggatttg caaggagctt cccaagttaa
     5401 agaagaaaca gacatagcag agcagaaatc agaaccaatg gaagtggatg aaaagaaacc
     5461 tgaagtgaaa gtagaagtta aagaggaaga agagagtagc agtaacggca cagcctctca
     5521 gtcaacatct ccttcgcagc cgcgcaaaaa aatctttaaa ccagaggagt tacgccaggc
     5581 cctcatgcca accctagaag cactgtatcg acaggaccca gagtcattac ctttccggca
     5641 gcctgtagat ccccagctcc tcggaattcc agactatttt gacatcgtaa agaatcccat
     5701 ggacctctcc accatcaagc ggaagctgga cacagggcaa taccaagagc cctggcagta
     5761 cgtggacgac gtctggctca tgttcaacaa tgcctggctc tataatcgca agacatcccg
     5821 agtctataag ttttgcagta agcttgcaga ggtctttgag caggaaattg accctgtcat
     5881 gcagtccctt ggatattgct gtggacgcaa gtatgagttt tccccacaga ctttgtgctg
     5941 ctatgggaag cagctgtgta ccattcctcg cgatgctgcc tactacagct atcagaatag
     6001 gtatcatttc tgtgagaagt gtttcacaga gatccagggc gagaatgtga ccctgggtga
     6061 cgacccttca cagccccaga cgacaatttc aaaggatcag tttgaaaaga agaaaaatga
     6121 taccttagac cccgaacctt tcgttgattg caaggagtgt ggccggaaga tgcatcagat
     6181 ttgcgttctg cactatgaca tcatttggcc ttcaggtttt gtgtgcgaca actgcttgaa
     6241 gaaaactggc agacctcgaa aagaaaacaa attcagtgct aagaggctgc agaccacaag
     6301 actgggaaac cacttggaag accgagtgaa caaatttttg cggcgccaga atcaccctga
     6361 agccggggag gtttttgtcc gagtggtggc cagctcagac aagacggtgg aggtcaagcc
     6421 cgggatgaag tcacggtttg tggattctgg ggaaatgtct gaatctttcc catatcgaac
     6481 caaagctctg tttgcttttg aggaaattga cggcgtggat gtctgctttt ttggaatgca
     6541 cgtccaagaa tacggctctg attgcccccc tccaaacacg aggcgtgtgt acatttctta
     6601 tctggatagt attcatttct tccggccacg ttgcctccgc acagccgttt accatgagat
     6661 ccttattgga tatttagagt atgtgaagaa attagggtat gtgacagggc acatctgggc
     6721 ctgtcctcca agtgaaggag atgattacat cttccattgc cacccacctg atcaaaaaat
     6781 acccaagcca aaacgactgc aggagtggta caaaaagatg ctggacaagg cgtttgcaga
     6841 gcggatcatc catgactaca aggatatttt caaacaagca actgaagaca ggctcaccag
     6901 tgccaaggaa ctgccctatt ttgaaggtga tttctggccc aatgtgttag aagagagcat
     6961 taaggaacta gaacaagaag aagaggagag gaaaaaggaa gagagcactg cagccagtga
     7021 aaccactgag ggcagtcagg gcgacagcaa gaatgccaag aagaagaaca acaagaaaac
     7081 caacaagaac aaaagcagca tcagccgcgc caacaagaag aagcccagca tgcccaacgt
     7141 gtccaatgac ctgtcccaga agctgtatgc caccatggag aagcacaagg aggtcttctt
     7201 cgtgatccac ctgcacgctg ggcctgtcat caacaccctg ccccccatcg tcgaccccga
     7261 ccccctgctc agctgtgacc tcatggatgg gcgcgacgcc ttcctcaccc tcgccagaga
     7321 caagcactgg gagttctcct ccttgcgccg ctccaagtgg tccacgctct gcatgctggt
     7381 ggagctgcac acccagggcc aggaccgctt tgtctacacc tgcaacgagt gcaagcacca
     7441 cgtggagacg cgctggcact gcactgtgtg cgaggactac gacctctgca tcaactgcta
     7501 taacacgaag agccatgccc ataagatggt gaagtggggg ctgggcctgg atgacgaggg
     7561 cagcagccag ggcgagccac agtcaaagag cccccaggag tcacgccggc tgagcatcca
     7621 gcgctgcatc cagtcgctgg tgcacgcgtg ccagtgccgc aacgccaact gctcgctgcc
     7681 atcctgccag aagatgaagc gggtggtgca gcacaccaag ggctgcaaac gcaagaccaa
     7741 cgggggctgc ccggtgtgca agcagctcat cgccctctgc tgctaccacg ccaagcactg
     7801 ccaagaaaac aaatgccccg tgcccttctg cctcaacatc aaacacaagc tccgccagca
     7861 gcagatccag caccgcctgc agcaggccca gctcatgcgc cggcggatgg ccaccatgaa
     7921 cacccgcaac gtgcctcagc agagtctgcc ttctcctacc tcagcaccgc ccgggacccc
     7981 cacacagcag cccagcacac cccagacgcc gcagccccct gcccagcccc aaccctcacc
     8041 cgtgagcatg tcaccagctg gcttccccag cgtggcccgg actcagcccc ccaccacggt
     8101 gtccacaggg aagcctacca gccaggtgcc ggccccccca cccccggccc agccccctcc
     8161 tgcagcggtg gaagcggctc ggcagatcga gcgtgaggcc cagcagcagc agcacctgta
     8221 ccgggtgaac atcaacaaca gcatgccccc aggacgcacg ggcatgggga ccccggggag
     8281 ccagatggcc cccgtgagcc tgaatgtgcc ccgacccaac caggtgagcg ggcccgtcat
     8341 gcccagcatg cctcccgggc agtggcagca ggcgcccctt ccccagcagc agcccatgcc
     8401 aggcttgccc aggcctgtga tatccatgca ggcccaggcg gccgtggctg ggccccggat
     8461 gcccagcgtg cagccaccca ggagcatctc acccagcgct ctgcaagacc tgctgcggac
     8521 cctgaagtcg cccagctccc ctcagcagca acagcaggtg ctgaacattc tcaaatcaaa
     8581 cccgcagcta atggcagctt tcatcaaaca gcgcacagcc aagtacgtgg ccaatcagcc
     8641 cggcatgcag ccccagcctg gcctccagtc ccagcccggc atgcaacccc agcctggcat
     8701 gcaccagcag cccagcctgc agaacctgaa tgccatgcag gctggcgtgc cgcggcccgg
     8761 tgtgcctcca cagcagcagg cgatgggagg cctgaacccc cagggccagg ccttgaacat
     8821 catgaaccca ggacacaacc ccaacatggc gagtatgaat ccacagtacc gagaaatgtt
     8881 acggaggcag ctgctgcagc agcagcagca acagcagcag caacaacagc agcaacagca
     8941 gcagcagcaa gggagtgccg gcatggctgg gggcatggcg gggcacggcc agttccagca
     9001 gcctcaagga cccggaggct acccaccggc catgcagcag cagcagcgca tgcagcagca
     9061 tctccccctc cagggcagct ccatgggcca gatggcggct cagatgggac agcttggcca
     9121 gatggggcag ccggggctgg gggcagacag cacccccaac atccagcaag ccctgcagca
     9181 gcggattctg cagcaacagc agatgaagca gcagattggg tccccaggcc agccgaaccc
     9241 catgagcccc cagcaacaca tgctctcagg acagccacag gcctcgcatc tccctggcca
     9301 gcagatcgcc acgtccctta gtaaccaggt gcggtctcca gcccctgtcc agtctccacg
     9361 gccccagtcc cagcctccac attccagccc gtcaccacgg atacagcccc agccttcgcc
     9421 acaccacgtc tcaccccaga ctggttcccc ccaccccgga ctcgcagtca ccatggccag
     9481 ctccatagat cagggacact tggggaaccc cgaacagagt gcaatgctcc cccagctgaa
     9541 cacccccagc aggagtgcgc tgtccagcga actgtccctg gtcggggaca ccacggggga
     9601 cacgctagag aagtttgtgg agggcttgct cgaggactac aaagaccatg acggtgatta
     9661 taaagatcat gacatcgact acaaggatga cgatgacaag tagcggccgc aaggatctgc
     9721 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     9781 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     9841 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     9901 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     9961 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
    10021 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
    10081 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
    10141 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
    10201 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgtcg
    10261 acatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
    10321 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
    10381 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
    10441 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
    10501 ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
    10561 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
    10621 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
    10681 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
    10741 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
    10801 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
    10861 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
    10921 cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg
    10981 cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc
    11041 cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg
    11101 acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg
    11161 gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg
    11221 gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa
    11281 aggaataggt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
    11341 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
    11401 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
    11461 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
    11521 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
    11581 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
    11641 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
    11701 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
    11761 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
    11821 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
    11881 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
    11941 aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga tctgcttttt
    12001 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    12061 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    12121 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    12181 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
    12241 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
    12301 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    12361 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
    12421 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    12481 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    12541 acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct gtgtgaaatt
    12601 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    12661 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    12721 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    12781 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    12841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    12901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    12961 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    13021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    13081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    13141 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    13201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    13261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    13321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    13381 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    13441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    13501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    13561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    13621 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    13681 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    13741 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    13801 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    13861 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    13921 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    13981 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    14041 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    14101 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    14161 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    14221 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    14281 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    14341 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    14401 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    14461 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    14521 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    14581 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    14641 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    14701 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    14761 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
    14821 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    14881 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
    14941 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    15001 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
    15061 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    15121 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    15181 cgacggccag tgccaagctg
//
