LOCUS       V040176                10169 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040176
VERSION     V040176
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..4582
                     /label="TFRC(NM_003234)"
                     /note="TFRC(NM_003234)"
                     /gene="TFRC"
     CDS             4604..4669
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4712..4923
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4936..5204
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5232..6257
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /note="confers resistance to hygromycin"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /transl_table=1
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E*"
                     /label="HygR"
     misc_feature    6264..6852
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6926..7159
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7231..7352
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7371..7506
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7544..7560)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7568..7584
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7592..7622)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7637..7658
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7946..8534)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8705..9565)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9566..9670)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10144..10160
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcc ggatccgcca ccatgatgga tcaagctaga tcagcattct ctaacttgtt
     2341 tggtggagaa ccattgtcat atacccggtt cagcctggct cggcaagtag atggcgataa
     2401 cagtcatgtg gagatgaaac ttgctgtaga tgaagaagaa aatgctgaca ataacacaaa
     2461 ggccaatgtc acaaaaccaa aaaggtgtag tggaagtatc tgctatggga ctattgctgt
     2521 gatcgtcttt ttcttgattg gatttatgat tggctacttg ggctattgta aaggggtaga
     2581 accaaaaact gagtgtgaga gactggcagg aaccgagtct ccagtgaggg aggagccagg
     2641 agaggacttc cctgcagcac gtcgcttata ttgggatgac ctgaagagaa agttgtcgga
     2701 gaaactggac agcacagact tcaccggcac catcaagctg ctgaatgaaa attcatatgt
     2761 ccctcgtgag gctggatctc aaaaagatga aaatcttgcg ttgtatgttg aaaatcaatt
     2821 tcgtgaattt aaactcagca aagtctggcg tgatcaacat tttgttaaga ttcaggtcaa
     2881 agacagcgct caaaactcgg tgatcatagt tgataagaac ggtagacttg tttacctggt
     2941 ggagaatcct gggggttatg tggcgtatag taaggctgca acagttactg gtaaactggt
     3001 ccatgctaat tttggtacta aaaaagattt tgaggattta tacactcctg tgaatggatc
     3061 tatagtgatt gtcagagcag ggaaaatcac ctttgcagaa aaggttgcaa atgctgaaag
     3121 cttaaatgca attggtgtgt tgatatacat ggaccagact aaatttccca ttgttaacgc
     3181 agaactttca ttctttggac atgctcatct ggggacaggt gacccttaca cacctggatt
     3241 cccttccttc aatcacactc agtttccacc atctcggtca tcaggattgc ctaatatacc
     3301 tgtccagaca atctccagag ctgctgcaga aaagctgttt gggaatatgg aaggagactg
     3361 tccctctgac tggaaaacag actctacatg taggatggta acctcagaaa gcaagaatgt
     3421 gaagctcact gtgagcaatg tgctgaaaga gataaaaatt cttaacatct ttggagttat
     3481 taaaggcttt gtagaaccag atcactatgt tgtagttggg gcccagagag atgcatgggg
     3541 ccctggagct gcaaaatccg gtgtaggcac agctctccta ttgaaacttg cccagatgtt
     3601 ctcagatatg gtcttaaaag atgggtttca gcccagcaga agcattatct ttgccagttg
     3661 gagtgctgga gactttggat cggttggtgc cactgaatgg ctagagggat acctttcgtc
     3721 cctgcattta aaggctttca cttatattaa tctggataaa gcggttcttg gtaccagcaa
     3781 cttcaaggtt tctgccagcc cactgttgta tacgcttatt gagaaaacaa tgcaaaatgt
     3841 gaagcatccg gttactgggc aatttctata tcaggacagc aactgggcca gcaaagttga
     3901 gaaactcact ttagacaatg ctgctttccc tttccttgca tattctggaa tcccagcagt
     3961 ttctttctgt ttttgcgagg acacagatta tccttatttg ggtaccacca tggacaccta
     4021 taaggaactg attgagagga ttcctgagtt gaacaaagtg gcacgagcag ctgcagaggt
     4081 cgctggtcag ttcgtgatta aactaaccca tgatgttgaa ttgaacctgg actatgagag
     4141 gtacaacagc caactgcttt catttgtgag ggatctgaac caatacagag cagacataaa
     4201 ggaaatgggc ctgagtttac agtggctgta ttctgctcgt ggagacttct tccgtgctac
     4261 ttccagacta acaacagatt tcgggaatgc tgagaaaaca gacagatttg tcatgaagaa
     4321 actcaatgat cgtgtcatga gagtggagta tcacttcctc tctccctacg tatctccaaa
     4381 agagtctcct ttccgacatg tcttctgggg ctccggctct cacacgctgc cagctttact
     4441 ggagaacttg aaactgcgta aacaaaataa cggtgctttt aatgaaacgc tgttcagaaa
     4501 ccagttggct ctagctactt ggactattca gggagctgca aatgccctct ctggtgacgt
     4561 ttgggacatt gacaatgagt ttggtggagg cggatcactc gaggactaca aagaccatga
     4621 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt agcggccgca
     4681 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4741 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     4801 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4861 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4921 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     4981 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     5041 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     5101 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     5161 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     5221 cctacgtcga catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     5281 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     5341 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     5401 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     5461 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     5521 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     5581 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     5641 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     5701 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     5761 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     5821 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     5881 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     5941 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     6001 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     6061 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     6121 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     6181 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     6241 cgagggcaaa ggaataggtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     6301 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6361 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6421 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6481 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6541 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6601 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     6661 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6721 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6781 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6841 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     6901 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat
     6961 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7021 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7081 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7141 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     7201 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     7261 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7321 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     7381 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7441 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7501 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg
     7561 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7621 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7681 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7741 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7801 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7861 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7921 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7981 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8041 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8101 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8161 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8221 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8281 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8341 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     8401 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8461 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8521 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8581 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8641 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8701 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8761 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8821 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8881 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8941 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9001 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9061 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9121 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9181 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9241 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9301 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9361 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9421 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9481 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9541 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9601 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9661 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     9721 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     9781 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     9841 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     9901 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     9961 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
    10021 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
    10081 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
    10141 gttgtaaaac gacggccagt gccaagctg
//
