LOCUS       V022869                12323 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022869
VERSION     V022869
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6417
                     /label="IGF1R(NM_000875)"
                     /note="IGF1R(NM_000875)"
                     /gene="IGF1R"
     promoter        6482..6693
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6706..6974
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7003..7012
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7033..7695
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7765..7818
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7819..8418
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8419..9006
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9080..9313
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9385..9506
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9525..9660
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9698..9714)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9722..9738
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9746..9776)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9791..9812
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10100..10688)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10859..11719)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11720..11824)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12298..12314
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga agtctggctc cggaggaggg
     2341 tccccgacct cgctgtgggg gctcctgttt ctctccgccg cgctctcgct ctggccgacg
     2401 agtggagaaa tctgcgggcc aggcatcgac atccgcaacg actatcagca gctgaagcgc
     2461 ctggagaact gcacggtgat cgagggctac ctccacatcc tgctcatctc caaggccgag
     2521 gactaccgca gctaccgctt ccccaagctc acggtcatta ccgagtactt gctgctgttc
     2581 cgagtggctg gcctcgagag cctcggagac ctcttcccca acctcacggt catccgcggc
     2641 tggaaactct tctacaacta cgccctggtc atcttcgaga tgaccaatct caaggatatt
     2701 gggctttaca acctgaggaa cattactcgg ggggccatca ggattgagaa aaatgctgac
     2761 ctctgttacc tctccactgt ggactggtcc ctgatcctgg atgcggtgtc caataactac
     2821 attgtgggga ataagccccc aaaggaatgt ggggacctgt gtccagggac catggaggag
     2881 aagccgatgt gtgagaagac caccatcaac aatgagtaca actaccgctg ctggaccaca
     2941 aaccgctgcc agaaaatgtg cccaagcacg tgtgggaagc gggcgtgcac cgagaacaat
     3001 gagtgctgcc accccgagtg cctgggcagc tgcagcgcgc ctgacaacga cacggcctgt
     3061 gtagcttgcc gccactacta ctatgccggt gtctgtgtgc ctgcctgccc gcccaacacc
     3121 tacaggtttg agggctggcg ctgtgtggac cgtgacttct gcgccaacat cctcagcgcc
     3181 gagagcagcg actccgaggg gtttgtgatc cacgacggcg agtgcatgca ggagtgcccc
     3241 tcgggcttca tccgcaacgg cagccagagc atgtactgca tcccttgtga aggtccttgc
     3301 ccgaaggtct gtgaggaaga aaagaaaaca aagaccattg attctgttac ttctgctcag
     3361 atgctccaag gatgcaccat cttcaagggc aatttgctca ttaacatccg acgggggaat
     3421 aacattgctt cagagctgga gaacttcatg gggctcatcg aggtggtgac gggctacgtg
     3481 aagatccgcc attctcatgc cttggtctcc ttgtccttcc taaaaaacct tcgcctcatc
     3541 ctaggagagg agcagctaga agggaattac tccttctacg tcctcgacaa ccagaacttg
     3601 cagcaactgt gggactggga ccaccgcaac ctgaccatca aagcagggaa aatgtacttt
     3661 gctttcaatc ccaaattatg tgtttccgaa atttaccgca tggaggaagt gacggggact
     3721 aaagggcgcc aaagcaaagg ggacataaac accaggaaca acggggagag agcctcctgt
     3781 gaaagtgacg tcctgcattt cacctccacc accacgtcga agaatcgcat catcataacc
     3841 tggcaccggt accggccccc tgactacagg gatctcatca gcttcaccgt ttactacaag
     3901 gaagcaccct ttaagaatgt cacagagtat gatgggcagg atgcctgcgg ctccaacagc
     3961 tggaacatgg tggacgtgga cctcccgccc aacaaggacg tggagcccgg catcttacta
     4021 catgggctga agccctggac tcagtacgcc gtttacgtca aggctgtgac cctcaccatg
     4081 gtggagaacg accatatccg tggggccaag agtgagatct tgtacattcg caccaatgct
     4141 tcagttcctt ccattccctt ggacgttctt tcagcatcga actcctcttc tcagttaatc
     4201 gtgaagtgga accctccctc tctgcccaac ggcaacctga gttactacat tgtgcgctgg
     4261 cagcggcagc ctcaggacgg ctacctttac cggcacaatt actgctccaa agacaaaatc
     4321 cccatcagga agtatgccga cggcaccatc gacattgagg aggtcacaga gaaccccaag
     4381 actgaggtgt gtggtgggga gaaagggcct tgctgcgcct gccccaaaac tgaagccgag
     4441 aagcaggccg agaaggagga ggctgaatac cgcaaagtct ttgagaattt cctgcacaac
     4501 tccatcttcg tgcccagacc tgaaaggaag cggagagatg tcatgcaagt ggccaacacc
     4561 accatgtcca gccgaagcag gaacaccacg gccgcagaca cctacaacat caccgacccg
     4621 gaagagctgg agacagagta ccctttcttt gagagcagag tggataacaa ggagagaact
     4681 gtcatttcta accttcggcc tttcacattg taccgcatcg atatccacag ctgcaaccac
     4741 gaggctgaga agctgggctg cagcgcctcc aacttcgtct ttgcaaggac tatgcccgca
     4801 gaaggagcag atgacattcc tgggccagtg acctgggagc caaggcctga aaactccatc
     4861 tttttaaagt ggccggaacc tgagaatccc aatggattga ttctaatgta tgaaataaaa
     4921 tacggatcac aagttgagga tcagcgagaa tgtgtgtcca gacaggaata caggaagtat
     4981 ggaggggcca agctaaaccg gctaaacccg gggaactaca cagcccggat tcaggccaca
     5041 tctctctctg ggaatgggtc gtggacagat cctgtgttct tctatgtcca ggccaaaaca
     5101 ggatatgaaa acttcatcca tctgatcatc gctctgcccg tcgctgtcct gttgatcgtg
     5161 ggagggttgg tgattatgct gtacgtcttc catagaaaga gaaataacag caggctgggg
     5221 aatggagtgc tgtatgcctc tgtgaacccg gagtacttca gcgctgctga tgtgtacgtt
     5281 cctgatgagt gggaggtggc tcgggagaag atcaccatga gccgggaact tgggcagggg
     5341 tcgtttggga tggtctatga aggagttgcc aagggtgtgg tgaaagatga acctgaaacc
     5401 agagtggcca ttaaaacagt gaacgaggcc gcaagcatgc gtgagaggat tgagtttctc
     5461 aacgaagctt ctgtgatgaa ggagttcaat tgtcaccatg tggtgcgatt gctgggtgtg
     5521 gtgtcccaag gccagccaac actggtcatc atggaactga tgacacgggg cgatctcaaa
     5581 agttatctcc ggtctctgag gccagaaatg gagaataatc cagtcctagc acctccaagc
     5641 ctgagcaaga tgattcagat ggccggagag attgcagacg gcatggcata cctcaacgcc
     5701 aataagttcg tccacagaga ccttgctgcc cggaattgca tggtagccga agatttcaca
     5761 gtcaaaatcg gagattttgg tatgacgcga gatatctatg agacagacta ttaccggaaa
     5821 ggagggaaag ggctgctgcc cgtgcgctgg atgtctcctg agtccctcaa ggatggagtc
     5881 ttcaccactt actcggacgt ctggtccttc ggggtcgtcc tctgggagat cgccacactg
     5941 gccgagcagc cctaccaggg cttgtccaac gagcaagtcc ttcgcttcgt catggagggc
     6001 ggccttctgg acaagccaga caactgtcct gacatgctgt ttgaactgat gcgcatgtgc
     6061 tggcagtata accccaagat gaggccttcc ttcctggaga tcatcagcag catcaaagag
     6121 gagatggagc ctggcttccg ggaggtctcc ttctactaca gcgaggagaa caagctgccc
     6181 gagccggagg agctggacct ggagccagag aacatggaga gcgtccccct ggacccctcg
     6241 gcctcctcgt cctccctgcc actgcccgac agacactcag gacacaaggc cgagaacggc
     6301 cccggccctg gggtgctggt cctccgcgcc agcttcgacg agagacagcc ttacgcccac
     6361 atgaacgggg gccgcaagaa cgagcgggcc ttgccgctgc cccagtcttc gacctgctga
     6421 ggatccctgc gatatccagc gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     6481 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     6541 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6601 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     6661 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     6721 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     6781 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     6841 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     6901 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     6961 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     7021 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     7081 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     7141 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     7201 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     7261 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     7321 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     7381 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     7441 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     7501 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     7561 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     7621 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     7681 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     7741 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     7801 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     7861 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7921 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7981 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     8041 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     8101 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     8161 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     8221 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     8281 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     8341 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     8401 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8461 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8521 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8581 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8641 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8701 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8761 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     8821 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8881 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8941 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct
     9001 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     9061 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt
     9121 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9181 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9241 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9301 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     9361 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     9421 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9481 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     9541 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     9601 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     9661 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa
     9721 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9781 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9841 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9901 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9961 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    10021 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10081 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10141 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10201 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10261 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10321 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10381 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10441 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10501 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    10561 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10621 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10681 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10741 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10801 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10861 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10921 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10981 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    11041 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    11101 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    11161 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    11221 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11281 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11341 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11401 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11461 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11521 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11581 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11641 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11701 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11761 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11821 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    11881 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
    11941 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
    12001 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
    12061 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    12121 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
    12181 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    12241 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    12301 aaacgacggc cagtgccaag ctg
//
