LOCUS       V039981                10207 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039981
VERSION     V039981
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4233
                     /label="ILDR2(NM_199351)"
                     /note="ILDR2(NM_199351)"
                     /gene="ILDR2"
     CDS             4240..4305
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4349..4560
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4573..4841
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4870..4879
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4900..5562
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5632..5685
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5686..6285
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6286..6874
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6948..7181
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7253..7374
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7414..7549
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7582..7598)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7606..7622
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7630..7660)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7675..7696
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7984..8572)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8743..9603)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9604..9708)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10182..10198
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg atagggtctt gctgaggtgg
     2341 atttctctct tctggctaac agccatggtc gaaggccttc aggtcacagt gcccgacaag
     2401 aagaaggtgg ccatgctctt ccagcccact gtgcttcgct gccacttctc aacatcctcc
     2461 catcagcctg cagttgtgca gtggaagttc aagtcctact gccaggatcg catgggagaa
     2521 tccttgggca tgtcctctac ccgggcccaa tctctcagca agagaaacct ggaatgggac
     2581 ccctacttgg attgtttgga cagcaggagg actgttcgag tagtagcttc aaaacagggc
     2641 tcgactgtca ccctgggaga tttctacagg ggcagagaga tcacgattgt tcatgatgca
     2701 gatcttcaaa ttggaaagct tatgtgggga gacagcggac tctattactg tattatcacc
     2761 accccagatg acctggaggg gaaaaatgag gactcagtgg aactgctggt gttgggcagg
     2821 acagggctgc ttgctgatct cttgcccagt tttgctgtgg agattatgcc agagtgggtg
     2881 tttgttggcc tggtgctcct gggcgtcttc ctcttcttcg tcctggtggg gatctgctgg
     2941 tgccagtgct gccctcacag ctgctgctgc tatgtccgct gcccatgctg cccagattcc
     3001 tgctgctgcc ctcaagcctt gtatgaagca gggaaagcag caaaggccgg gtaccctccc
     3061 tctgtctccg gtgtccccgg cccttactcc atcccctctg tccctttggg aggagccccc
     3121 tcatctggca tgctgatgga caagccgcat ccacctccct tggcaccaag tgactccact
     3181 ggaggaagcc acagtgttcg caaaggttac cggatccagg ctgacaaaga gagagactcc
     3241 atgaaggtcc tgtactatgt tgagaaggag ctggctcagt ttgatccagc cagaaggatg
     3301 agaggcagat ataacaacac catctcagaa ctcagctccc tacatgagga ggacagcaat
     3361 ttccgccagt ctttccatca gatgagaagc aagcagttcc ctgtgtctgg ggacttggag
     3421 agcaatcctg actattggtc aggtgtcatg ggaggcagca gtggggcaag ccgcgggccc
     3481 tcagccatgg agtataacaa agaggatcga gagagcttca ggcacagcca gccgcgctcc
     3541 aagtcggaga tgctgtcgcg gaagaacttc gccacggggg tgccggccgt ttccatggac
     3601 gagctggcgg ccttcgctga ctcctacggc cagcggcccc gccgggcaga cggcaacagt
     3661 cacgaggcgc ggggcgggag ccgcttcgag cgctcggagt cgcgggcgca cagcggcttc
     3721 taccaggacg actccttgga ggagtactac ggtcagcgca gccgcagccg cgagcccctg
     3781 accgatgctg accgcggctg ggccttcagc cccgcgcgcc gcagacccgc cgaggacgcg
     3841 cacctgccgc ggctggtgag ccgcacgcca ggcaccgcac ccaaatacga ccactcgtac
     3901 ctgggcagcg cgcgggagcg ccaggcgcgg cccgagggcg ccagccgcgg tggcagcctg
     3961 gagacgccat ccaagcggag cgcgcagctc ggcccgcgca gcgcctccta ctacgcttgg
     4021 tcgccgcccg gcacctacaa ggccggctcg tcgcaggacg accaggagga cgcgtccgac
     4081 gacgcgctgc cgccctacag cgagctggag ctgacccgcg gcccgtccta ccgcggccgc
     4141 gacctgccct accacagcaa ctcggagaag aagaggaaaa aggagcccgc caagaaaacc
     4201 aatgactttc caaccaggat gtcccttgtg gtcggatccg actacaaaga ccatgacggt
     4261 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     4321 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4381 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     4441 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     4501 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     4561 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     4621 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     4681 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     4741 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     4801 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     4861 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     4921 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     4981 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     5041 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     5101 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     5161 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     5221 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     5281 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     5341 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     5401 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     5461 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     5521 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     5581 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     5641 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     5701 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5761 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5821 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5881 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5941 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6001 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6061 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6121 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6181 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6241 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     6301 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6361 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6421 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6481 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6541 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6601 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6661 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     6721 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6781 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6841 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     6901 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6961 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     7021 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7081 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7141 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7201 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     7261 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7321 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7381 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7441 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7501 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     7561 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7621 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7681 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7741 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7801 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7861 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7921 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7981 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8041 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8101 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8161 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8221 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8281 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8341 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8401 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8461 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8521 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8581 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8641 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8701 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8761 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8821 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8881 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8941 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9001 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9061 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9121 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9181 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9241 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9301 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9361 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9421 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9481 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9541 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9601 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9661 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9721 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     9781 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     9841 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     9901 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     9961 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    10021 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    10081 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    10141 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    10201 caagctg
//
