LOCUS       V025810                12016 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025810
VERSION     V025810
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6042
                     /label="IRS1(NM_005544)"
                     /note="IRS1(NM_005544)"
                     /gene="IRS1"
     CDS             6049..6114
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6158..6369
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6382..6650
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6679..6688
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6709..7371
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7441..7494
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7495..8094
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8095..8683
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8757..8990
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9062..9183
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9223..9358
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9391..9407)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9415..9431
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9439..9469)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9484..9505
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9793..10381)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10552..11412)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11413..11517)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11991..12007
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgagccctcc ggagagcgat
     2341 ggcttctcgg acgtgcgcaa ggtgggctac ctgcgcaaac ccaagagcat gcacaaacgc
     2401 ttcttcgtac tgcgcgcggc cagcgaggct gggggcccgg cgcgcctcga gtactacgag
     2461 aacgagaaga agtggcggca caagtcgagc gcccccaaac gctcgatccc ccttgagagc
     2521 tgcttcaaca tcaacaagcg ggctgactcc aagaacaagc acctggtggc tctctacacc
     2581 cgggacgagc actttgccat cgcggcggac agcgaggccg agcaagacag ctggtaccag
     2641 gctctcctac agctgcacaa ccgtgctaag ggccaccacg acggagctgc ggccctcggg
     2701 gcgggaggtg gtgggggcag ctgcagcggc agctccggcc ttggtgaggc tggggaggac
     2761 ttgagctacg gtgacgtgcc cccaggaccc gcattcaaag aggtctggca agtgatcctg
     2821 aagcccaagg gcctgggtca gacaaagaac ctgattggta tctaccgcct ttgcctgacc
     2881 agcaagacca tcagcttcgt gaagctgaac tcggaggcag cggccgtggt gctgcagctg
     2941 atgaacatca ggcgctgtgg ccactcggaa aacttcttct tcatcgaggt gggccgttct
     3001 gccgtgacgg ggcccgggga gttctggatg caggtggatg actctgtggt ggcccagaac
     3061 atgcacgaga ccatcctgga ggccatgcgg gccatgagtg atgagttccg ccctcgcagc
     3121 aagagccagt cctcgtccaa ctgctctaac cccatcagcg tccccctgcg ccggcaccat
     3181 ctcaacaatc ccccgcccag ccaggtgggg ctgacccgcc gatcacgcac tgagagcatc
     3241 accgccacct ccccggccag catggtgggc gggaagccag gctccttccg tgtccgcgcc
     3301 tccagtgacg gcgaaggcac catgtcccgc ccagcctcgg tggacggcag ccctgtgagt
     3361 cccagcacca acagaaccca cgcccaccgg catcggggca gcgcccggct gcaccccccg
     3421 ctcaaccaca gccgctccat ccccatgccg gcttcccgct gctcgccttc ggccaccagc
     3481 ccggtcagtc tgtcgtccag tagcaccagt ggccatggct ccacctcgga ttgtctcttc
     3541 ccacggcgat ctagtgcttc ggtgtctggt tcccccagcg atggcggttt catctcctcg
     3601 gatgagtatg gctccagtcc ctgcgatttc cggagttcct tccgcagtgt cactccggat
     3661 tccctgggcc acaccccacc agcccgcggt gaggaggagc taagcaacta tatctgcatg
     3721 ggtggcaagg ggccctccac cctgaccgcc cccaacggtc actacatttt gtctcggggt
     3781 ggcaatggcc accgctgcac cccaggaaca ggcttgggca cgagtccagc cttggctggg
     3841 gatgaagcag ccagtgctgc agatctggat aatcggttcc gaaagagaac tcactcggca
     3901 ggcacatccc ctaccattac ccaccagaag accccgtccc agtcctcagt ggcttccatt
     3961 gaggagtaca cagagatgat gcctgcctac ccaccaggag gtggcagtgg aggccgactg
     4021 ccgggacaca ggcactccgc cttcgtgccc acccgctcct acccagagga gggtctggaa
     4081 atgcacccct tggagcgtcg gggggggcac caccgcccag acagctccac cctccacacg
     4141 gatgatggct acatgcccat gtccccaggg gtggccccag tgcccagtgg ccgaaagggc
     4201 agtggagact atatgcccat gagccccaag agcgtatctg ccccacagca gatcatcaat
     4261 cccatcagac gccatcccca gagagtggac cccaatggct acatgatgat gtcccccagc
     4321 ggtggctgct ctcctgacat tggaggtggc cccagcagca gcagcagcag cagcaacgcc
     4381 gtcccttccg ggaccagcta tggaaagctg tggacaaacg gggtaggggg ccaccactct
     4441 catgtcttgc ctcaccccaa acccccagtg gagagcagcg gtggtaagct cttaccttgc
     4501 acaggtgact acatgaacat gtcaccagtg ggggactcca acaccagcag cccctccgac
     4561 tgctactacg gccctgagga cccccagcac aagccagtcc tctcctacta ctcattgcca
     4621 agatccttta agcacaccca gcgccccggg gagccggagg agggtgcccg gcatcagcac
     4681 ctccgccttt ccactagctc tggtcgcctt ctctatgctg caacagcaga tgattcttcc
     4741 tcttccacca gcagcgacag cctgggtggg ggatactgcg gggctaggct ggagcccagc
     4801 cttccacatc cccaccatca ggttctgcag ccccatctgc ctcgaaaggt ggacacagct
     4861 gctcagacca atagccgcct ggcccggccc acgaggctgt ccctggggga tcccaaggcc
     4921 agcaccttac ctcgggcccg agagcagcag cagcagcagc agcccttgct gcaccctcca
     4981 gagcccaaga gcccggggga atatgtcaat attgaatttg ggagtgatca gtctggctac
     5041 ttgtctggcc cggtggcttt ccacagctca ccttctgtca ggtgtccatc ccagctccag
     5101 ccagctccca gagaggaaga gactggcact gaggagtaca tgaagatgga cctggggccg
     5161 ggccggaggg cagcctggca ggagagcact ggggtcgaga tgggcagact gggccctgca
     5221 cctcccgggg ctgctagcat ttgcaggcct acccgggcag tgcccagcag ccggggtgac
     5281 tacatgacca tgcagatgag ttgtccccgt cagagctacg tggacacctc gccagctgcc
     5341 cctgtaagct atgctgacat gcgaacaggc attgctgcag aggaggtgag cctgcccagg
     5401 gccaccatgg ctgctgcctc ctcatcctca gcagcctctg cttccccgac tgggcctcaa
     5461 ggggcagcag agctggctgc ccactcgtcc ctgctggggg gcccacaagg acctgggggc
     5521 atgagcgcct tcacccgggt gaacctcagt cctaaccgca accagagtgc caaagtgatc
     5581 cgtgcagacc cacaagggtg ccggcggagg catagctccg agactttctc ctcaacaccc
     5641 agtgccaccc gggtgggcaa cacagtgccc tttggagcgg gggcagcagt agggggcggt
     5701 ggcggtagca gcagcagcag cgaggatgtg aaacgccaca gctctgcttc ctttgagaat
     5761 gtgtggctga ggcctgggga gcttggggga gcccccaagg agccagccaa actgtgtggg
     5821 gctgctgggg gtttggagaa tggtcttaac tacatagacc tggatttggt caaggacttc
     5881 aaacagtgcc ctcaggagtg cacccctgaa ccgcagcctc ccccaccccc accccctcat
     5941 caacccctgg gcagcggtga gagcagctcc acccgccgct caagtgagga tttaagcgcc
     6001 tatgccagca tcagtttcca gaagcagcca gaggaccgtc agggatccga ctacaaagac
     6061 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagcgg
     6121 ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     6181 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc
     6241 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     6301 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     6361 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
     6421 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
     6481 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
     6541 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
     6601 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
     6661 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag
     6721 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga
     6781 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg
     6841 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc
     6901 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac
     6961 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac
     7021 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc
     7081 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg
     7141 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac
     7201 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg
     7261 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag
     7321 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc
     7381 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc
     7441 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc
     7501 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     7561 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     7621 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     7681 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     7741 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     7801 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     7861 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     7921 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     7981 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     8041 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa
     8101 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     8161 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8221 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8281 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8341 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8401 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8461 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8521 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8581 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8641 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     8701 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     8761 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     8821 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8881 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8941 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     9001 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     9061 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9121 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9181 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     9241 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9301 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9361 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     9421 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9481 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9541 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     9601 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9661 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9721 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9781 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9841 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9901 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9961 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10021 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10081 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10141 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10201 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    10261 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10321 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10381 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10441 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10501 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10561 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10621 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10681 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10741 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10801 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10861 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10921 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10981 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11041 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11101 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11161 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11221 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11281 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11341 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11401 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11461 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    11521 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    11581 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    11641 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    11701 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    11761 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    11821 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
    11881 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
    11941 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
    12001 ggccagtgcc aagctg
//
