LOCUS       V043632                10720 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043632
VERSION     V043632
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5574
                     /label="ITGA6(NM_001079818)"
                     /note="ITGA6(NM_001079818)"
                     /gene="ITGA6"
     CDS             5581..5646
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5690..5901
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5914..6182
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6209..6808
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6815..7403
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7477..7710
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7782..7903
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7922..8057
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8095..8111)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8119..8135
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8143..8173)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8188..8209
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8497..9085)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9256..10116)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10117..10221)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10695..10711
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggccgcc gccgggcagc tgtgcttgct ctacctgtcg
     2341 gcggggctcc tgtcccggct cggcgcagcc ttcaacttgg acactcggga ggacaacgtg
     2401 atccggaaat atggagaccc cgggagcctc ttcggcttct cgctggccat gcactggcaa
     2461 ctgcagcccg aggacaagcg gctgttgctc gtgggggccc cgcgggcaga agcgcttcca
     2521 ctgcagagag ccaacagaac gggagggctg tacagctgcg acatcaccgc ccgggggcca
     2581 tgcacgcgga tcgagtttga taacgatgct gaccccacgt cagaaagcaa ggaagatcag
     2641 tggatggggg tcaccgtcca gagccaaggt ccagggggca aggtcgtgac atgtgctcac
     2701 cgatatgaaa aaaggcagca tgttaatacg aagcaggaat cccgagacat ctttgggcgg
     2761 tgttatgtcc tgagtcagaa tctcaggatt gaagacgata tggatggggg agattggagc
     2821 ttttgtgatg ggcgattgag aggccatgag aaatttggct cttgccagca aggtgtagca
     2881 gctactttta ctaaagactt tcattacatt gtatttggag ccccgggtac ttataactgg
     2941 aaagggattg ttcgtgtaga gcaaaagaat aacacttttt ttgacatgaa catctttgaa
     3001 gatgggcctt atgaagttgg tggagagact gagcatgatg aaagtctcgt tcctgttcct
     3061 gctaacagtt acttaggttt ttctttggac tcagggaaag gtattgtttc taaagatgag
     3121 atcacttttg tatctggtgc tcccagagcc aatcacagtg gagccgtggt tttgctgaag
     3181 agagacatga agtctgcaca tctcctccct gagcacatat tcgatggaga aggtctggcc
     3241 tcttcatttg gctatgatgt ggcggtggtg gacctcaaca aggatgggtg gcaagatata
     3301 gttattggag ccccacagta ttttgataga gatggagaag ttggaggtgc agtgtatgtc
     3361 tacatgaacc agcaaggcag atggaataat gtgaagccaa ttcgtcttaa tggaaccaaa
     3421 gattctatgt ttggcattgc agtaaaaaat attggagata ttaatcaaga tggctaccca
     3481 gatattgcag ttggagctcc gtatgatgac ttgggaaagg tttttatcta tcatggatct
     3541 gcaaatggaa taaataccaa accaacacag gttctcaagg gtatatcacc ttattttgga
     3601 tattcaattg ctggaaacat ggaccttgat cgaaattcct accctgatgt tgctgttggt
     3661 tccctctcag attcagtaac tattttcaga tcccggcctg tgattaatat tcagaaaacc
     3721 atcacagtaa ctcctaacag aattgacctc cgccagaaaa cagcgtgtgg ggcgcctagt
     3781 gggatatgcc tccaggttaa atcctgtttt gaatatactg ctaaccccgc tggttataat
     3841 ccttcaatat caattgtggg cacacttgaa gctgaaaaag aaagaagaaa atctgggcta
     3901 tcctcaagag ttcagtttcg aaaccaaggt tctgagccca aatatactca agaactaact
     3961 ctgaagaggc agaaacagaa agtgtgcatg gaggaaaccc tgtggctaca ggataatatc
     4021 agagataaac tgcgtcccat tcccataact gcctcagtgg agatccaaga gccaagctct
     4081 cgtaggcgag tgaattcact tccagaagtt cttccaattc tgaattcaga tgaacccaag
     4141 acagctcata ttgatgttca cttcttaaaa gagggatgtg gagacgacaa tgtatgtaac
     4201 agcaacctta aactagaata taaattttgc acccgagaag gaaatcaaga caaattttct
     4261 tatttaccaa ttcaaaaagg tgtaccagaa ctagttctaa aagatcagaa ggatattgct
     4321 ttagaaataa cagtgacaaa cagcccttcc aacccaagga atcccacaaa agatggcgat
     4381 gacgcccatg aggctaaact gattgcaacg tttccagaca ctttaaccta ttctgcatat
     4441 agagaactga gggctttccc tgagaaacag ttgagttgtg ttgccaacca gaatggctcg
     4501 caagctgact gtgagctcgg aaatcctttt aaaagaaatt caaatgtcac tttttatttg
     4561 gttttaagta caactgaagt cacctttgac accccagatc tggatattaa tctgaagtta
     4621 gaaacaacaa gcaatcaaga taatttggct ccaattacag ctaaagcaaa agtggttatt
     4681 gaactgcttt tatcggtctc gggagttgct aaaccttccc aggtgtattt tggaggtaca
     4741 gttgttggcg agcaagctat gaaatctgaa gatgaagtgg gaagtttaat agagtatgaa
     4801 ttcagggtaa taaacttagg taaacctctt acaaacctcg gcacagcaac cttgaacatt
     4861 cagtggccaa aagaaattag caatgggaaa tggttgcttt atttggtgaa agtagaatcc
     4921 aaaggattgg aaaaggtaac ttgtgagcca caaaaggaga taaactccct gaacctaacg
     4981 gagtctcaca actcaagaaa gaaacgggaa attactgaaa aacagataga tgataacaga
     5041 aaattttctt tatttgctga aagaaaatac cagactctta actgtagcgt gaacgtgaac
     5101 tgtgtgaaca tcagatgccc gctgcggggg ctggacagca aggcgtctct tattttgcgc
     5161 tcgaggttat ggaacagcac atttctagag gaatattcca aactgaacta cttggacatt
     5221 ctcatgcgag ccttcattga tgtgactgct gctgccgaaa atatcaggct gccaaatgca
     5281 ggcactcagg ttcgagtgac tgtgtttccc tcaaagactg tagctcagta ttcgggagta
     5341 ccttggtgga tcatcctagt ggctattctc gctgggatct tgatgcttgc tttattagtg
     5401 tttatactat ggaagtgtgg attctttaaa cgctctaggt acgatgacag tgttccccga
     5461 taccatgctg taaggatccg gaaagaagag cgagagatca aagatgaaaa gtatattgat
     5521 aaccttgaaa aaaaacagtg gatcacaaag tggaacgaaa atgaaagcta ctcaggatcc
     5581 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     5641 gacaagtagc ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     5701 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     5761 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     5821 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     5881 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     5941 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     6001 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     6061 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     6121 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     6181 tccaagctgt gaccggcgcc tacgctagat gaccgagtac aagcccacgg tgcgcctcgc
     6241 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6301 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6361 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6421 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6481 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6541 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6601 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6661 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     6721 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     6781 gtgcatgacc cgcaagcccg gtgcctgagt cgacaatcaa cctctggatt acaaaatttg
     6841 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6901 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6961 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     7021 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     7081 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     7141 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     7201 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     7261 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     7321 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     7381 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     7441 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7501 gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7561 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7621 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7681 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     7741 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7801 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7861 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7921 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7981 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8041 aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata
     8101 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8161 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8221 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8281 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8341 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8401 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8461 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8521 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8581 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8641 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8701 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8761 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8821 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8881 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8941 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9001 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9061 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9121 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9181 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9241 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9301 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9361 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9421 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9481 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9541 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9601 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9661 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9721 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9781 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9841 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9901 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9961 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10021 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10081 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10141 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10201 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    10261 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    10321 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    10381 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    10441 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    10501 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    10561 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    10621 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    10681 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
