LOCUS       V018451                10581 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018451
VERSION     V018451
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4690
                     /label="ITGB1(NM_002211)"
                     /note="ITGB1(NM_002211)"
                     /gene="ITGB1"
     promoter        4740..4951
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4964..5232
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5261..5270
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5291..5953
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6023..6076
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6077..6676
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6677..7264
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7338..7571
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7643..7764
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7783..7918
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7956..7972)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7980..7996
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8004..8034)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8049..8070
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8358..8946)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9117..9977)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9978..10082)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10556..10572
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatga atttacaacc aattttctgg attggactga tcagttcagt
     2341 ttgctgtgtg tttgctcaaa cagatgaaaa tagatgttta aaagcaaatg ccaaatcatg
     2401 tggagaatgt atacaagcag ggccaaattg tgggtggtgc acaaattcaa catttttaca
     2461 ggaaggaatg cctacttctg cacgatgtga tgatttagaa gccttaaaaa agaagggttg
     2521 ccctccagat gacatagaaa atcccagagg ctccaaagat ataaagaaaa ataaaaatgt
     2581 aaccaaccgt agcaaaggaa cagcagagaa gctcaagcca gaggatatta ctcagatcca
     2641 accacagcag ttggttttgc gattaagatc aggggagcca cagacattta cattaaaatt
     2701 caagagagct gaagactatc ccattgacct ctactacctt atggacctgt cttactcaat
     2761 gaaagacgat ttggagaatg taaaaagtct tggaacagat ctgatgaatg aaatgaggag
     2821 gattacttcg gacttcagaa ttggatttgg ctcatttgtg gaaaagactg tgatgcctta
     2881 cattagcaca acaccagcta agctcaggaa cccttgcaca agtgaacaga actgcaccag
     2941 cccatttagc tacaaaaatg tgctcagtct tactaataaa ggagaagtat ttaatgaact
     3001 tgttggaaaa cagcgcatat ctggaaattt ggattctcca gaaggtggtt tcgatgccat
     3061 catgcaagtt gcagtttgtg gatcactgat tggctggagg aatgttacac ggctgctggt
     3121 gttttccaca gatgccgggt ttcactttgc tggagatggg aaacttggtg gcattgtttt
     3181 accaaatgat ggacaatgtc acctggaaaa taatatgtac acaatgagcc attattatga
     3241 ttatccttct attgctcacc ttgtccagaa actgagtgaa aataatattc agacaatttt
     3301 tgcagttact gaagaatttc agcctgttta caaggagctg aaaaacttga tccctaagtc
     3361 agcagtagga acattatctg caaattctag caatgtaatt cagttgatca ttgatgcata
     3421 caattccctt tcctcagaag tcattttgga aaacggcaaa ttgtcagaag gcgtaacaat
     3481 aagttacaaa tcttactgca agaacggggt gaatggaaca ggggaaaatg gaagaaaatg
     3541 ttccaatatt tccattggag atgaggttca atttgaaatt agcataactt caaataagtg
     3601 tccaaaaaag gattctgaca gctttaaaat taggcctctg ggctttacgg aggaagtaga
     3661 ggttattctt cagtacatct gtgaatgtga atgccaaagc gaaggcatcc ctgaaagtcc
     3721 caagtgtcat gaaggaaatg ggacatttga gtgtggcgcg tgcaggtgca atgaagggcg
     3781 tgttggtaga cattgtgaat gcagcacaga tgaagttaac agtgaagaca tggatgctta
     3841 ctgcaggaaa gaaaacagtt cagaaatctg cagtaacaat ggagagtgcg tctgcggaca
     3901 gtgtgtttgt aggaagaggg ataatacaaa tgaaatttat tctggcaaat tctgcgagtg
     3961 tgataatttc aactgtgata gatccaatgg cttaatttgt ggaggaaatg gtgtttgcaa
     4021 gtgtcgtgtg tgtgagtgca accccaacta cactggcagt gcatgtgact gttctttgga
     4081 tactagtact tgtgaagcca gcaacggaca gatctgcaat ggccggggca tctgcgagtg
     4141 tggtgtctgt aagtgtacag atccgaagtt tcaagggcaa acgtgtgaga tgtgtcagac
     4201 ctgccttggt gtctgtgctg agcataaaga atgtgttcag tgcagagcct tcaataaagg
     4261 agaaaagaaa gacacatgca cacaggaatg ttcctatttt aacattacca aggtagaaag
     4321 tcgggacaaa ttaccccagc cggtccaacc tgatcctgtg tcccattgta aggagaagga
     4381 tgttgacgac tgttggttct attttacgta ttcagtgaat gggaacaacg aggtcatggt
     4441 tcatgttgtg gagaatccag agtgtcccac tggtccagac atcattccaa ttgtagctgg
     4501 tgtggttgct ggaattgttc ttattggcct tgcattactg ctgatatgga agcttttaat
     4561 gataattcat gacagaaggg agtttgctaa atttgaaaag gagaaaatga atgccaaatg
     4621 ggacacgggt gaaaatccta tttataagag tgccgtaaca actgtggtca atccgaagta
     4681 tgagggaaaa tgaggatccg cggccgcaag gatctgcgat cgctccggtg cccgtcagtg
     4741 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     4801 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     4861 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     4921 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
     4981 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     5041 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     5101 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     5161 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     5221 ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga
     5281 gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga
     5341 gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat
     5401 gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta
     5461 cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat
     5521 caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca
     5581 cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg
     5641 caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac
     5701 cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt
     5761 cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag
     5821 cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga
     5881 ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc
     5941 catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg
     6001 acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga
     6061 ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     6121 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     6181 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     6241 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     6301 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     6361 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     6421 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     6481 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6541 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6601 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6661 caagcccggt gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6721 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6781 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6841 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6901 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6961 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7021 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7081 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7141 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7201 gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg atctcccttt ggccgcctcc
     7261 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7321 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt
     7381 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7441 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7501 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7561 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7621 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7681 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7741 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     7801 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7861 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7921 gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7981 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     8041 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8101 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8161 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8221 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8281 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8341 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8401 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8461 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8521 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8581 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8641 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8701 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8761 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     8821 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8881 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8941 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9001 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9061 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9121 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9181 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9241 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9301 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9361 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9421 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9481 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9541 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9601 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9661 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9721 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9781 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9841 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9901 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9961 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10021 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10081 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
    10141 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
    10201 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
    10261 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
    10321 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
    10381 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
    10441 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    10501 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    10561 acgacggcca gtgccaagct g
//
