LOCUS V037076 8821 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037076 VERSION V037076 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1804..1920 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2010..2213 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2302..3675 /label="KAT8(NM_032188)" /note="KAT8(NM_032188)" /gene="KAT8" CDS 3682..3747 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" promoter 3791..4002 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 4015..4283 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4310..4909 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4916..5504 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5578..5811 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5883..6004 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6023..6158 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6196..6212) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6220..6236 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6244..6274) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6289..6310 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6598..7186) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7357..8217) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8218..8322) /gene="bla" /label="AmpR promoter" primer_bind 8796..8812 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agagctagcg aattcgccac catggcggca cagggagctg ctgcggcggt tgcggcgggg 2341 acttcagggg tcgcggggga gggcgagccc gggcccgggg agaatgcggc cgctgagggg 2401 accgccccat ccccgggccg cgtctctccg ccgaccccgg cgcgcggcga gccggaagtc 2461 acggtggaga tcggagaaac gtacctgtgc cggcgaccgg atagcacctg gcattctgct 2521 gaagtgatcc agtctcgagt gaacgaccag gagggccgag aggaattcta tgtacactac 2581 gtgggcttta accggcggct ggacgagtgg gtagacaaga accggctggc gctgaccaag 2641 acagtgaagg atgctgtaca gaagaactca gagaagtacc tgagcgagct cgcagagcag 2701 cctgagcgca agatcactcg caaccaaaag cgcaagcatg atgagatcaa ccatgtgcag 2761 aagacttatg cagagatgga ccccaccaca gcagccttgg agaaggagca tgaggcgatc 2821 accaaggtga agtatgtgga caagatccac atcgggaact acgaaattga tgcctggtat 2881 ttctcaccat tccccgaaga ctatgggaaa cagcccaagc tctggctctg cgagtactgc 2941 ctcaagtaca tgaaatatga gaagagctac cgcttccact tgggtcagtg ccagtggcgg 3001 cagccccccg ggaaagagat ctaccgcaag agcaacatct ccgtgtacga agttgatggc 3061 aaagaccata agatttactg tcagaacctg tgtctgctgg ccaagctttt cctggaccat 3121 aagacactgt actttgacgt ggagccgttc gtcttttaca tcctgactga ggtggaccgg 3181 cagggggccc acattgttgg ctacttctcc aaggagaagg agtccccgga tggaaacaat 3241 gtggcctgca tcctgacctt gcccccctac caacgccgcg gctacgggaa gttcctcatc 3301 gctttcagtt atgagctctc caagctggag agcacagtcg gctccccgga gaagccactg 3361 tctgacctgg gcaagctcag ctaccgcagc tactggtcct gggtgctgct agagatcctg 3421 cgggacttcc ggggcacact gtccatcaag gacctcagcc agatgaccag tatcacccaa 3481 aatgacatca tcagtaccct gcaatccctc aatatggtca agtactggaa gggccagcac 3541 gtgatctgtg tcacacccaa gctggtggag gagcacctca aaagtgccca gtataagaaa 3601 ccacccatca cagtggactc cgtctgcctc aagtgggcac cccccaagca caagcaagtc 3661 aagctctcca agaagggatc cgactacaaa gaccatgacg gtgattataa agatcatgac 3721 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt 3781 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 3841 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 3901 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 3961 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 4021 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 4081 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 4141 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 4201 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 4261 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga tgaccgagta 4321 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc 4381 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga 4441 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 4501 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 4561 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc 4621 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 4681 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 4741 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc 4801 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt 4861 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca 4921 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 4981 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 5041 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 5101 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 5161 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 5221 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 5281 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg 5341 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 5401 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 5461 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac 5521 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 5581 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct 5641 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 5701 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 5761 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 5821 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 5881 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 5941 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 6001 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc 6061 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 6121 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc 6181 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 6241 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 6301 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 6361 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 6421 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 6481 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 6541 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 6601 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 6661 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 6721 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 6781 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 6841 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 6901 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 6961 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 7021 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 7081 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 7141 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 7201 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 7261 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 7321 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 7381 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 7441 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 7501 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 7561 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 7621 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 7681 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 7741 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 7801 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 7861 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 7921 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 7981 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 8041 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 8101 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 8161 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 8221 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 8281 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 8341 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 8401 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 8461 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 8521 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 8581 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 8641 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 8701 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 8761 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 8821 g //