LOCUS       V038897                13360 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038897
VERSION     V038897
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7386
                     /label="KDM5A(NM_001042603)"
                     /note="KDM5A(NM_001042603)"
                     /gene="KDM5A"
     CDS             7393..7458
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7502..7713
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7726..7994
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      8023..8032
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             8053..8715
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8785..8838
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8839..9438
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9439..10027
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10101..10334
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10406..10527
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10567..10702
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10735..10751)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10759..10775
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10783..10813)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10828..10849
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11137..11725)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11896..12756)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12757..12861)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13335..13351
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg cgggcgtggg gccggggggc
     2341 tacgcggcgg agttcgtgcc accgccagag tgccccgtct ttgagccgag ttgggaggag
     2401 ttcacagatc cgctcagctt tatcggccgc atccggcctt tggcggagaa aaccggcatc
     2461 tgcaaaattc ggccgcccaa ggactggcag cctccatttg cctgtgaagt aaaaagcttt
     2521 cgtttcactc caagagtcca gcgcctgaat gaacttgagg caatgaccag agtgagattg
     2581 gatttcttgg atcaactagc aaaattttgg gaacttcaag gatctactct gaagatccct
     2641 gtggtagaga gaaaaatcct ggatctgtat gctttgagca agattgttgc cagcaaagga
     2701 ggttttgaaa tggtcaccaa agagaagaaa tggtctaaag tgggtagtcg cttgggatat
     2761 ctgccaggaa aaggaactgg gtctcttttg aagtcacatt atgaaagaat tctctaccca
     2821 tatgagcttt tccagtctgg tgtgagcctt atgggtgtgc agatgcctaa tttagatctt
     2881 aaagaaaaag tggagcctga ggttctcagc actgataccc aaacttcccc agagccaggc
     2941 acaaggatga acattctgcc gaagagaaca agacgtgtga agactcagtc agaatctgga
     3001 gatgtgagta gaaacacgga actgaagaaa cttcagattt ttggggctgg gcccaaggtt
     3061 gtgggcttgg caatgggaac aaaagataaa gaagatgagg tcacccgaag acgaaaagtt
     3121 accaacaggt cagacgcatt taacatgcaa atgagacaac ggaaaggcac tctctctgtt
     3181 aactttgttg atctctatgt ttgtatgttt tgtggtcggg gaaacaatga agataaattg
     3241 cttttgtgtg atggatgtga tgacagctat catacatttt gtctaattcc tccactacct
     3301 gatgtgccca aaggagactg gaggtgtcct aaatgtgtcg ccgaggaatg tagcaaacct
     3361 cgagaagcct ttggatttga acaagctgta cgagagtata cacttcagag ctttggagag
     3421 atggcagata attttaagtc tgattatttt aatatgccag tccatatggt tcccacagaa
     3481 ctagtagaaa aggaattttg gcggctggta agcagcattg aagaagatgt tattgtggaa
     3541 tatggagcag atatctcctc aaaagacttt ggaagtggat ttccggtgaa ggatgggcgg
     3601 agaaagattc tgccagaaga agaggaatat gcactttctg gttggaattt gaataacatg
     3661 cctgtcctgg aacagtctgt tcttgcacat attaatgtgg acatctctgg tatgaaagtg
     3721 ccgtggctct atgtgggaat gtgcttctct tctttttgct ggcacattga ggatcactgg
     3781 agttattcca tcaactactt gcactggggg gagccaaaga catggtatgg tgtgccatct
     3841 catgctgcag agcaactgga ggaggtgatg agagagctgg cccccgagtt atttgaatcc
     3901 cagcctgatc ttctgcatca gttagttacc atcatgaacc ccaacgtgct aatggagcat
     3961 ggtgtgcctg tgtacaggac caatcagtgt gctggcgagt ttgttgtgac atttcctcgt
     4021 gcctatcact ctggatttaa ccagggctac aactttgctg aagctgtgaa cttctgtact
     4081 gctgactggt tgcccattgg acgtcaatgt gtaaatcatt accgacgcct aaggcgccac
     4141 tgtgtctttt cacacgagga actaattttc aagatggcag cagatccaga atgcttagat
     4201 gtggggctgg ctgccatggt ctgcaaagaa ttgactctca tgactgaaga agaaacacga
     4261 ttaagagagt ctgttgtaca gatgggtgtc ctgatgtcag aagaagaagt gtttgaactt
     4321 gttcctgatg atgagcggca gtgttcagca tgcagaacca catgttttct ctctgctctc
     4381 acatgttcct gtaatcctga gcggcttgta tgtctctacc atccaactga tctgtgcccc
     4441 tgccccatgc agaagaaatg tcttagatat cgctacccat tagaagacct cccttctctg
     4501 ctatatggtg taaaagtcag ggcacagtcc tatgacactt gggtcagtcg tgttacagaa
     4561 gcattgtctg ctaacttcaa ccacaaaaaa gatttgattg aattgcgagt aatgctggaa
     4621 gatgctgagg ataggaaata cccagagaat gatctctttc gaaaactcag ggatgctgta
     4681 aaagaagctg agacctgtgc ttctgtggct cagctgcttc tgagcaaaaa gcagaaacac
     4741 agacagagcc cagatagtgg gaggactcgg accaaactga cagtggaaga attgaaggcc
     4801 tttgtccaac aactttttag tcttccgtgt gtcatcagcc aagctcggca agtaaagaat
     4861 ctgctagatg atgtggaaga gtttcatgaa cgtgctcagg aggccatgat ggatgaaacc
     4921 ccagattctt ccaaactcca gatgttgata gatatgggct ctagtctcta tgtggaactc
     4981 cctgaattac cacgactgaa gcaagagcta caacaggctc ggtggttgga cgaagtaaga
     5041 ctgaccttat cagatccgca acaagtcact ttggatgtca tgaagaagct gatagactct
     5101 ggggtagggt tggcacccca ccatgctgtg gagaaagcaa tggctgaact acaggagctc
     5161 cttacagtct ctgaacgatg ggaagaaaag gctaaggtct gcctacaggc aagaccgagg
     5221 cacagtgtgg caagtttaga aagcattgtg aatgaagcca agaacattcc agcctttcta
     5281 cccaatgtgt tgtccttgaa agaagcctta caaaaggctc gagaatggac cgctaaagtg
     5341 gaagctattc agagtggcag caattacgct tatttggagc agcttgagag cttgtctgcg
     5401 aaaggacgcc ctattcctgt gcgtcttgaa gcactgccgc aagtggaatc acaggtagca
     5461 gcagcacggg catggagaga acggactggg cggacgtttc ttaagaagaa ttctagccat
     5521 acattgttac aggtgctgag cccccggacc gacattggtg tatatgggag tggcaaaaat
     5581 aggaggaaaa aagtaaaaga actaatagaa aaagaaaaag aaaaggatct ggacctggag
     5641 cctctgagtg atctggagga aggattggag gaaaccagag atacagccat ggtggtggca
     5701 gttttcaaag aacgggagca aaaagagatt gaagccatgc attctctcag agcagccaac
     5761 ctagccaaga tgacaatggt ggaccgcata gaagaagtaa aattttgcat ttgccgcaag
     5821 acagccagtg ggtttatgct acagtgtgag ctctgcaaag actggttcca taacagctgt
     5881 gttcctcttc ctaaatcaag ttcccaaaaa aaaggatcca gctggcaagc taaagaagta
     5941 aaattccttt gccctctttg tatgcggtct cgaaggccca ggctagagac tattctgtca
     6001 ctcctggtat cccttcagaa gttgcccgta cggttgcctg aaggagaggc cctgcagtgt
     6061 ttgacagaac gtgctatgag ttggcaagat agagcgcggc aggctctagc cacagatgaa
     6121 ctatcctctg ccctggccaa actatctgtg ttgagccagc gtatggtgga acaggcggct
     6181 cgagaaaaaa ctgaaaagat catcagtgca gaactccaaa aagcagctgc caatccagac
     6241 ttacagggac acttacctag tttccagcag tctgctttta accgggtggt gagcagtgtg
     6301 tcatcttctc ctcgacaaac aatggactat gatgatgaag aaacagactc tgatgaagac
     6361 attcgagaga catatggcta cgacatgaag gacacagcca gtgtgaagtc ctctagtagt
     6421 cttgaaccca atcttttttg tgatgaagag attcccatca aatccgagga ggtggtgacc
     6481 cacatgtgga cagcaccttc attttgtgca gagcatgctt attcttctgc ttctaagagt
     6541 tgttctcaag gttctagcac cccaaggaaa caacctcgga agagcccttt ggtgccccga
     6601 agtttggaac ctccagtgct ggagttgtca cctggagcta aggcacaact ggaagaactt
     6661 atgatggttg gagatctcct ggaagtatct ctggacgaga ctcaacacat atggcggatt
     6721 ttgcaggcca cacacccacc ctctgaagac agattcttgc atatcatgga ggatgacagc
     6781 atggaagaga aaccactaaa agtgaaagga aaggactctt cagagaagaa acggaaacgg
     6841 aagctagaaa aggtagagca actttttgga gaaggaaaac agaagtccaa ggagttaaag
     6901 aaaatggaca aacctagaaa gaagaaatta aaattaggtg cagacaaatc aaaggagctg
     6961 aataaactgg ccaagaaact agcaaaagaa gaagagagaa agaaaaagaa ggagaaggct
     7021 gctgcagcca aagttgaact tgtgaaagag agcactgaaa agaaaagaga gaaaaaggtg
     7081 ctggacatcc cctcaaagta tgactggtca ggagcagagg agtctgatga tgagaatgct
     7141 gtgtgcgcag cacagaactg ccaaaggccc tgcaaggaca aggtagactg ggtacaatgt
     7201 gatggtggct gtgatgagtg gtttcatcaa gtttgtgtgg gtgtatctcc agaaatggct
     7261 gaaaatgaag attacatctg tataaactgt gcaaagaagc aggggccagt tagcccaggt
     7321 ccagcaccac ctccttcctt cataatgagc tacaaactac caatggagga tcttaaagag
     7381 accagtggat ccgactacaa agaccatgac ggtgattata aagatcatga catcgactac
     7441 aaggatgacg atgacaagta gcggccgcga aggatctgcg atcgctccgg tgcccgtcag
     7501 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     7561 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     7621 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     7681 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     7741 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     7801 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     7861 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     7921 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     7981 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac
     8041 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg
     8101 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag
     8161 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
     8221 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc
     8281 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg
     8341 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg
     8401 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc
     8461 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc
     8521 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag
     8581 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg
     8641 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc
     8701 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc
     8761 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg
     8821 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     8881 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     8941 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     9001 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     9061 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     9121 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     9181 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     9241 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     9301 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     9361 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     9421 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     9481 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     9541 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     9601 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     9661 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     9721 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     9781 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     9841 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     9901 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     9961 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
    10021 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
    10081 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct
    10141 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    10201 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    10261 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    10321 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
    10381 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
    10441 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10501 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
    10561 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    10621 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    10681 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata
    10741 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    10801 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    10861 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    10921 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    10981 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    11041 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    11101 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    11161 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11221 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    11281 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    11341 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    11401 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    11461 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    11521 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    11581 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    11641 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    11701 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    11761 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    11821 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    11881 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    11941 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    12001 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    12061 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    12121 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    12181 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    12241 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    12301 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    12361 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    12421 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    12481 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    12541 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    12601 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    12661 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    12721 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    12781 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    12841 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    12901 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
    12961 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    13021 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    13081 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
    13141 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
    13201 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    13261 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    13321 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
