LOCUS       V033873                10162 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033873
VERSION     V033873
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4188
                     /label="KEAP1(NM_203500)"
                     /note="KEAP1(NM_203500)"
                     /gene="KEAP1"
     CDS             4195..4260
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4304..4515
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4528..4796
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      4825..4834
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             4855..5517
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5587..5640
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5641..6240
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6241..6829
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6903..7136
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7208..7329
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7369..7504
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7537..7553)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7561..7577
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7585..7615)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7630..7651
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7939..8527)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8698..9558)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9559..9663)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10137..10153
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc agccagatcc caggcctagc
     2341 ggggctgggg cctgctgccg attcctgccc ctgcagtcac agtgccctga gggggcaggg
     2401 gacgcggtga tgtacgcctc cactgagtgc aaggcggagg tgacgccctc ccagcatggc
     2461 aaccgcacct tcagctacac cctggaggat cataccaagc aggcctttgg catcatgaac
     2521 gagctgcggc tcagccagca gctgtgtgac gtcacactgc aggtcaagta ccaggatgca
     2581 ccggccgccc agttcatggc ccacaaggtg gtgctggcct catccagccc tgtcttcaag
     2641 gccatgttca ccaacgggct gcgggagcag ggcatggagg tggtgtccat tgagggtatc
     2701 caccccaagg tcatggagcg cctcattgaa ttcgcctaca cggcctccat ctccatgggc
     2761 gagaagtgtg tcctccacgt catgaacggt gctgtcatgt accagatcga cagcgttgtc
     2821 cgtgcctgca gtgacttcct ggtgcagcag ctggacccca gcaatgccat cggcatcgcc
     2881 aacttcgctg agcagattgg ctgtgtggag ttgcaccagc gtgcccggga gtacatctac
     2941 atgcattttg gggaggtggc caagcaagag gagttcttca acctgtccca ctgccaactg
     3001 gtgaccctca tcagccggga cgacctgaac gtgcgctgcg agtccgaggt cttccacgcc
     3061 tgcatcaact gggtcaagta cgactgcgaa cagcgacggt tctacgtcca ggcgctgctg
     3121 cgggccgtgc gctgccactc gttgacgccg aacttcctgc agatgcagct gcagaagtgc
     3181 gagatcctgc agtccgactc ccgctgcaag gactacctgg tcaagatctt cgaggagctc
     3241 accctgcaca agcccacgca ggtgatgccc tgccgggcgc ccaaggtggg ccgcctgatc
     3301 tacaccgcgg gcggctactt ccgacagtcg ctcagctacc tggaggctta caaccccagt
     3361 gacggcacct ggctccggtt ggcggacctg caggtgccgc ggagcggcct ggccggctgc
     3421 gtggtgggcg ggctgttgta cgccgtgggc ggcaggaaca actcgcccga cggcaacacc
     3481 gactccagcg ccctggactg ttacaacccc atgaccaatc agtggtcgcc ctgcgccccc
     3541 atgagcgtgc cccgtaaccg catcggggtg ggggtcatcg atggccacat ctatgccgtc
     3601 ggcggctccc acggctgcat ccaccacaac agtgtggaga ggtatgagcc agagcgggat
     3661 gagtggcact tggtggcccc aatgctgaca cgaaggatcg gggtgggcgt ggctgtcctc
     3721 aatcgtctcc tttatgccgt ggggggcttt gacgggacaa accgccttaa ttcagctgag
     3781 tgttactacc cagagaggaa cgagtggcga atgatcacag caatgaacac catccgaagc
     3841 ggggcaggcg tctgcgtcct gcacaactgt atctatgctg ctgggggcta tgatggtcag
     3901 gaccagctga acagcgtgga gcgctacgat gtggaaacag agacgtggac tttcgtagcc
     3961 cccatgaagc accggcgaag tgccctgggg atcactgtcc accaggggag aatctacgtc
     4021 cttggaggct atgatggtca cacgttcctg gacagtgtgg agtgttacga cccagataca
     4081 gacacctgga gcgaggtgac ccgaatgaca tcgggccgga gtggggtggg cgtggctgtc
     4141 accatggagc cctgccggaa gcagattgac cagcagaact gtacctgtgg atccgactac
     4201 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
     4261 tagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4321 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     4381 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     4441 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     4501 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     4561 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     4621 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     4681 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     4741 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     4801 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc
     4861 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc
     4921 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc
     4981 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc
     5041 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac
     5101 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac
     5161 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag
     5221 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac
     5281 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc
     5341 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc
     5401 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac
     5461 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga
     5521 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga
     5581 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     5641 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     5701 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     5761 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     5821 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     5881 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5941 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6001 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6061 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6121 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6181 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     6241 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6301 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6361 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6421 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6481 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6541 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6601 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     6661 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6721 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6781 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt
     6841 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6901 actggaaggg ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc
     6961 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7021 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7081 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     7141 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7201 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7261 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7321 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     7381 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7441 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7501 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     7561 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7621 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7681 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7741 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7801 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7861 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7921 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7981 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8041 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8101 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8161 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8221 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8281 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8341 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8401 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8461 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8521 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8581 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8641 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8701 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8761 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8821 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8881 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8941 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9001 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9061 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9121 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9181 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9241 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9301 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9361 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9421 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9481 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9541 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9601 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9661 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     9721 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     9781 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     9841 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     9901 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     9961 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    10021 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    10081 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    10141 aacgacggcc agtgccaagc tg
//
