LOCUS       V035583                10433 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035583
VERSION     V035583
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4434
                     /label="KIF2A(NM_004520)"
                     /note="KIF2A(NM_004520)"
                     /gene="KIF2A"
     CDS             4441..4467
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4471..4497
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4501..4527
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4592..4803
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4816..5084
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5113..5122
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5143..5805
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5875..5928
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5929..6528
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6529..7116
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7190..7423
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7495..7616
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7635..7770
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7808..7824)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7832..7848
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7856..7886)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7901..7922
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8210..8798)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8969..9829)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9830..9934)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10408..10424
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg caacggccaa cttcggcaag
     2341 atccagatcg ggatttacgt ggagatcaag cgcagcgatg gccgaataca tcaagcaatg
     2401 gtaacatctt taaatgaaga taatgaaagt gtaactgttg aatggataga aaatggagat
     2461 acaaaaggca aagagattga cctggagagc atcttttcac ttaaccctga ccttgttcct
     2521 gatgaagaaa ttgaacccag tccagaaaca cctccacctc cagcatcctc agccaaagta
     2581 aacaaaattg taaagaatcg acggactgta gcttctatta agaatgaccc tccttcaaga
     2641 gataatagag tggttggttc agcacgtgca cggcccagtc aatttcctga acagtcttcc
     2701 tctgcacaac agaatggtag tgtttcagat atatctccag ttcaagctgc aaaaaaggaa
     2761 tttggacccc cttcacgtag aaaatctaat tgtgtgaaag aagtagaaaa actgcaagaa
     2821 aaacgagaga aaaggagatt gcaacagcaa gaacttagag aaaaaagagc ccaggacgtt
     2881 gatgctacaa acccaaatta tgaaattatg tgtatgatca gagactttag aggaagtttg
     2941 gattatagac cattaacaac agcagatcct attgatgaac ataggatatg tgtgtgtgta
     3001 agaaaacgac cactcaataa aaaagaaact caaatgaaag atcttgatgt aatcacaatt
     3061 cctagtaaag atgttgtgat ggtacatgaa ccaaaacaaa aagtagattt aacaaggtac
     3121 ctagaaaacc aaacatttcg ttttgattat gcctttgatg actcagctcc taatgaaatg
     3181 gtttacaggt ttactgctag accactagtg gaaactatat ttgaaagggg aatggctaca
     3241 tgctttgctt atgggcagac tggaagtgga aaaactcata ctatgggtgg tgacttttca
     3301 ggaaagaacc aagattgttc taaaggaatt tatgcattag cagctcgaga tgtcttttta
     3361 atgctaaaga agccaaacta taagaagcta gaacttcaag tatatgcaac cttctttgaa
     3421 atttatagtg gaaaggtgtt tgacttgcta aacaggaaaa caaaattaag agttctagaa
     3481 gatggaaaac agcaggttca agtggtggga ttacaggaac gggaggtcaa atgtgttgaa
     3541 gatgtactga aactcattga cataggcaac agttgcagaa catccggtca aacatctgca
     3601 aatgcacatt catctcggag ccatgcagtg tttcagatta ttcttagaag gaaaggaaaa
     3661 ctacatggca aattttctct cattgatttg gctggaaatg aaagaggagc tgatacttcc
     3721 agtgcggaca ggcaaactag gcttgaaggt gctgaaatta ataaaagcct tttagcactc
     3781 aaggagtgca tcagagcctt aggtagaaat aaacctcata ctcctttccg tgcaagtaaa
     3841 ctcactcagg tgttaagaga ttctttcata ggtgaaaact ctcgtacctg catgattgcc
     3901 acaatctctc caggaatggc atcctgtgaa aatactctta atacattaag atatgcaaat
     3961 agggtcaaag aattgactgt agatccaact gctgctggtg atgttcgtcc aataatgcac
     4021 catccaccaa accagattga tgacttagag acacagtggg gtgtggggag ttcccctcag
     4081 agagatgatc taaaacttct ttgtgaacaa aatgaagaag aagtctctcc acagttgttt
     4141 actttccacg aagctgtttc acaaatggta gaaatggaag aacaagttgt agaagatcac
     4201 agggcagtgt tccaggaatc tattcggtgg ttagaagatg aaaaggccct cttagagatg
     4261 actgaagaag tagattatga tgtcgattca tatgctacac aacttgaagc tattcttgag
     4321 caaaaaatag acattttaac tgaactgcgg gataaagtga aatctttccg tgcagctcta
     4381 caagaggagg aacaagccag caagcaaatc aacccgaaga gaccccgtgc ccttctcgag
     4441 tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga ttatgctagc
     4501 tatccttatg atgtcccaga ctacgcatag ggatccctgc gatatccagc gcggccgcga
     4561 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4621 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
     4681 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4741 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4801 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc
     4861 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     4921 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     4981 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
     5041 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg
     5101 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag
     5161 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc
     5221 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc
     5281 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc
     5341 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc
     5401 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc
     5461 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc
     5521 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat
     5581 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc
     5641 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac
     5701 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc
     5761 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag
     5821 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc
     5881 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac
     5941 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     6001 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     6061 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     6121 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     6181 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     6241 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     6301 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     6361 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     6421 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     6481 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg
     6541 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6601 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6661 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6721 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6781 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6841 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6901 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     6961 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7021 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct
     7081 cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact
     7141 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7201 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac
     7261 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7321 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7381 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7441 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     7501 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7561 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7621 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7681 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7741 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt
     7801 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7861 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7921 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7981 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8041 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8101 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8161 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8221 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8281 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8341 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8401 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8461 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8521 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8581 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8641 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8701 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8761 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8821 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8881 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8941 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9001 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9061 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9121 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9181 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9241 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9301 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9361 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9421 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9481 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9541 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9601 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9661 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9721 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9781 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9841 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9901 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9961 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    10021 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    10081 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10141 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    10201 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10261 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    10321 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    10381 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
