LOCUS       V045131                10514 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045131
VERSION     V045131
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4491
                     /label="KIF2C(NM_006845)"
                     /note="KIF2C(NM_006845)"
                     /gene="KIF2C"
     CDS             4522..4548
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4552..4578
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4582..4608
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4673..4884
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4897..5165
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5194..5203
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5224..5886
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5956..6009
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6010..6609
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6610..7197
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7271..7504
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7576..7697
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7716..7851
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7889..7905)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7913..7929
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7937..7967)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7982..8003
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8291..8879)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9050..9910)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9911..10015)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10489..10505
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ccatggactc gtcgcttcag
     2341 gcccgcctgt ttcccggtct cgctatcaag atccaacgca gtaatggttt aattcacagt
     2401 gccaatgtaa ggactgtgaa cttggagaaa tcctgtgttt cagtggaatg ggcagaagga
     2461 ggtgccacaa agggcaaaga gattgatttt gatgatgtgg ctgcaataaa cccagaactc
     2521 ttacagcttc ttcccttaca tccgaaggac aatctgccct tgcaggaaaa tgtaacaatc
     2581 cagaaacaaa aacggagatc cgtcaactcc aaaattcctg ctccaaaaga aagtcttcga
     2641 agccgctcca ctcgcatgtc cactgtctca gagcttcgca tcacggctca ggagaatgac
     2701 atggaggtgg agctgcctgc agctgcaaac tcccgcaagc agttttcagt tcctcctgcc
     2761 cccactaggc cttcctgccc tgcagtggct gaaataccat tgaggatggt cagcgaggag
     2821 atggaagagc aagtccattc catccgaggc agctcttctg caaaccctgt gaactcagtt
     2881 cggaggaaat catgtcttgt gaaggaagtg gaaaaaatga agaacaagcg agaagagaag
     2941 aaggcccaga actctgaaat gagaatgaag agagctcagg agtatgacag tagttttcca
     3001 aactgggaat ttgcccgaat gattaaagaa tttcgggcta ctttggaatg tcatccactt
     3061 actatgactg atcctatcga agagcacaga atatgtgtct gtgttaggaa acgcccactg
     3121 aataagcaag aattggccaa gaaagaaatt gatgtgattt ccattcctag caagtgtctc
     3181 ctcttggtac atgaacccaa gttgaaagtg gacttaacaa agtatctgga gaaccaagca
     3241 ttctgctttg actttgcatt tgatgaaaca gcttcgaatg aagttgtcta caggttcaca
     3301 gcaaggccac tggtacagac aatctttgaa ggtggaaaag caacttgttt tgcatatggc
     3361 cagacaggaa gtggcaagac acatactatg ggcggagacc tctctgggaa agcccagaat
     3421 gcatccaaag ggatctatgc catggcctcc cgggacgtct tcctcctgaa gaatcaaccc
     3481 tgctaccgga agttgggcct ggaagtctat gtgacattct tcgagatcta caatgggaag
     3541 ctgtttgacc tgctcaacaa gaaggccaag ctgcgcgtgc tggaggacgg caagcaacag
     3601 gtgcaagtgg tggggctgca ggagcatctg gttaactctg ctgatgatgt catcaagatg
     3661 atcgacatgg gcagcgcctg cagaacctct gggcagacat ttgccaactc caattcctcc
     3721 cgctcccacg cgtgcttcca aattattctt cgagctaaag ggagaatgca tggcaagttc
     3781 tctttggtag atctggcagg gaatgagcga ggcgcggaca cttccagtgc tgaccggcag
     3841 acccgcatgg agggcgcaga aatcaacaag agtctcttag ccctgaagga gtgcatcagg
     3901 gccctgggac agaacaaggc tcacaccccg ttccgtgaga gcaagctgac acaggtgctg
     3961 agggactcct tcattgggga gaactctagg acttgcatga ttgccacgat ctcaccaggc
     4021 ataagctcct gtgaatatac tttaaacacc ctgagatatg cagacagggt caaggagctg
     4081 agcccccaca gtgggcccag tggagagcag ttgattcaaa tggaaacaga agagatggaa
     4141 gcctgctcta acggggcgct gattccaggc aatttatcca aggaagagga ggaactgtct
     4201 tcccagatgt ccagctttaa cgaagccatg actcagatca gggagctgga ggagaaggct
     4261 atggaagagc tcaaggagat catacagcaa ggaccagact ggcttgagct ctctgagatg
     4321 accgagcagc cagactatga cctggagacc tttgtgaaca aagcggaatc tgctctggcc
     4381 cagcaagcca agcatttctc agccctgcga gatgtcatca aggccttgcg cctggccatg
     4441 cagctggaag agcaggctag cagacaaata agcagcaaga aacggcccca gggtggaggc
     4501 ggatcaggag gcggtggatc ttacccatac gacgtacctg attatgcaac ttatccttat
     4561 gatgtacctg attatgctag ctatccttat gatgtcccag actacgcata gggatccctg
     4621 cgatatccag cgcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     4681 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     4741 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     4801 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     4861 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     4921 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     4981 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     5041 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     5101 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     5161 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc
     5221 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
     5281 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
     5341 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
     5401 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
     5461 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
     5521 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
     5581 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
     5641 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
     5701 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
     5761 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
     5821 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
     5881 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
     5941 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     6001 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6061 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     6121 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6181 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6241 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6301 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6361 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     6421 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     6481 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     6541 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     6601 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6661 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6721 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6781 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6841 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6901 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6961 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     7021 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7081 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7141 ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg
     7201 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7261 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt
     7321 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7381 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7441 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7501 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7561 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7621 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7681 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     7741 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     7801 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt
     7861 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7921 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7981 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8101 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8161 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8221 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8281 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8341 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8401 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8461 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8521 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8581 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8641 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8701 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8761 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8821 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8881 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8941 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9001 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9061 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9121 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9181 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9241 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9301 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9361 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9421 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9481 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9541 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9601 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9661 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9721 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9781 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9841 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9901 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9961 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10021 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    10081 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
    10141 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    10201 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
    10261 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    10321 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
    10381 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    10441 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    10501 ccagtgccaa gctg
//
