LOCUS       V043940                10855 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043940
VERSION     V043940
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3018
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3055..5709
                     /label="KMT5B(NM_017635)"
                     /note="KMT5B(NM_017635)"
                     /gene="KMT5B"
     CDS             5716..5781
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5825..6036
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6049..6317
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6344..6943
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6950..7538
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7612..7845
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7917..8038
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8057..8192
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8230..8246)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8254..8270
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8278..8308)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8323..8344
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8632..9220)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9391..10251)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10252..10356)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10830..10846
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcagccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2761 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2821 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3001 atggacgagc tgtacaaggg aggaggggga agcggtggag gcggttctct cgagatgaag
     3061 tggttgggag aatccaagaa catggtggtg aatggcagga gaaatggagg caagttgtct
     3121 aatgaccatc agcagaatca atcaaaatta cagcacacgg ggaaggacac cctgaaggct
     3181 ggcaaaaatg cagtcgagag gaggtcgaac agatgtaatg gtaactcggg atttgaagga
     3241 cagagtcgct atgtaccatc ctctggaatg tccgccaagg aactctgtga aaatgatgac
     3301 ctagcaacca gtttggttct tgatccctat ttaggttttc aaacacacaa aatgaatact
     3361 agcgcctttc cttcgaggag ctcaaggcat ttttcaaaat ctgacagttt ttctcacaac
     3421 aaccctgtga gatttaggcc tattaaagga aggcaggaag aactaaagga agtaattgaa
     3481 cgttttaaga aagatgaaca cttggagaaa gccttcaaat gtttgacttc aggcgaatgg
     3541 gcacggcact attttctcaa caagaataaa atgcaggaga aattattcaa agaacatgta
     3601 tttatttatt tgcgaatgtt tgcaactgac agtggatttg aaatattgcc atgtaataga
     3661 tactcatcag aacaaaatgg agccaaaata gttgcaacaa aagagtggaa acgaaatgac
     3721 aaaatagaat tactggtggg ttgtattgcc gaactttcag aaattgagga gaacatgcta
     3781 cttagacatg gagaaaacga cttcagtgtc atgtactcca caaggaaaaa ctgtgctcaa
     3841 ctctggctgg gtcctgctgc gtttataaac catgattgca gacctaattg taagtttgtg
     3901 tcaactggtc gagatacagc atgtgtgaag gctctaagag acattgaacc tggagaagaa
     3961 atttcttgtt attatggaga tgggttcttt ggagaaaata atgagttctg cgagtgttac
     4021 acttgcgaaa gacggggcac tggtgctttt aaatccagag tgggactgcc tgcgcctgct
     4081 cctgttatca atagcaaata tggactcaga gaaacagata aacgtttaaa taggcttaaa
     4141 aagttaggtg acagcagcaa aaattcagac agtcaatctg tcagctctaa cactgatgca
     4201 gataccactc aggaaaaaaa caatgcaact tctaaccgaa aatcttcagt tggcgtaaaa
     4261 aagaatagca agagcagaac gttaacgagg caatctatgt caagaattcc agcttcttcc
     4321 aactctacct catctaagct aactcatata aataattcca gggtaccaaa gaaactgaag
     4381 aagcctgcaa agcctttact ttcaaagata aaattgagaa atcattgcaa gcggctggag
     4441 caaaagaatg cttcaagaaa actcgaaatg ggaaacttag tactgaaaga gcctaaagta
     4501 gttctgtata aaaatttgcc cattaaaaaa gataaggagc cagagggacc agcccaagcc
     4561 gcagttgcca gcgggtgctt gactagacac gcggcgagag aacacagaca gaatcctgtg
     4621 agaggtgctc attcgcaggg ggagagctcg ccctgcacct acataactcg gcggtcagtg
     4681 aggacaagaa caaatctgaa ggaggcctct gacatcaagc ttgaaccaaa tacgttgaat
     4741 ggctataaaa gcagtgtgac ggaaccttgc cccgacagtg gtgaacagct gcagccagct
     4801 cctgtgctgc aggaggaaga actggctcat gagactgcac aaaaagggga ggcaaagtgt
     4861 cataagagtg acacaggcat gtccaaaaag aagtcacgac aaggaaaact tgtgaaacag
     4921 tttgcaaaaa tagaggaatc tactccagtg cacgattctc ctggaaaaga cgacgcggta
     4981 ccagatttga tgggtcccca ttctgaccag ggtgagcaca gtggcactgt gggcgtgcct
     5041 gtgagctaca cagactgtgc tccttcaccc gtcggttgtt cagttgtgac atcagatagc
     5101 ttcaaaacaa aagacagctt tagaactgca aaaagtaaaa agaagaggcg aatcacaagg
     5161 tatgatgcac agttaatcct agaaaataac tctgggattc ccaaattgac tcttcgtagg
     5221 cgtcatgata gcagcagcaa aacaaatgac caagagaatg atggaatgaa ctcttccaaa
     5281 ataagcatca agttaagcaa agaccatgac aacgataaca atctctatgt agcaaagctt
     5341 aataatggat ttaactcagg atcaggcagt agttctacaa aattaaaaat ccagctaaaa
     5401 cgagatgagg aaaatagggg gtcttataca gaggggcttc atgaaaatgg ggtgtgctgc
     5461 agtgatcctc tttctctctt ggagtctcga atggaggtgg atgactatag tcagtatgag
     5521 gaagaaagta cagatgattc ctcctcttct gagggcgatg aagaggagga tgactatgat
     5581 gatgactttg aagacgattt tattcctctt cctccagcta agcgcttgag gttaatagtt
     5641 ggaaaagact ctatagatat tgacatttct tcaaggagaa gagaagatca gtctttaagg
     5701 cttaatgccg gatccgacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     5761 tacaaggatg acgatgacaa gtagcggccg cgaaggatct gcgatcgctc cggtgcccgt
     5821 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5881 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5941 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     6001 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     6061 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     6121 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     6181 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     6241 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     6301 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagatgaccg agtacaagcc
     6361 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     6421 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     6481 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6541 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6601 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6661 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6721 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6781 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     6841 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6901 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca atcaacctct
     6961 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7021 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7081 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7141 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7201 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7261 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7321 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     7381 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7441 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7501 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga
     7561 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7621 taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag
     7681 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7741 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7801 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     7861 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     7921 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7981 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8041 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8101 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8161 ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc
     8221 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8281 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8341 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8401 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8461 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8521 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8581 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8641 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8701 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8761 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8821 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8881 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8941 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     9001 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     9061 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     9121 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     9181 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     9241 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9301 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9361 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9421 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9481 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9541 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9601 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9661 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9721 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9781 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9841 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9901 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9961 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    10021 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    10081 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    10141 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10201 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    10261 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10321 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10381 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    10441 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    10501 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    10561 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
    10621 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    10681 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    10741 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
    10801 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
