LOCUS       V024458                11174 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024458
VERSION     V024458
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5217
                     /label="Kif23(NM_001405055)"
                     /note="Kif23(NM_001405055)"
                     /gene="Kif23"
     CDS             5224..5289
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5333..5544
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5557..5825
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5854..5863
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5884..6546
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6616..6669
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6670..7269
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7270..7857
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7931..8164
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8236..8357
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8376..8511
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8549..8565)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8573..8589
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8597..8627)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8642..8663
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8951..9539)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9710..10570)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10571..10675)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11149..11165
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga agtcagcgaa ggctaagacg
     2341 gtcagaaaac ctgtaataaa aaaaggatct cagacaaacc ttaaagatcc agtgggggtg
     2401 tactgtaggg ttcgccccct gagctttcct gaccaagaat gctgtgtgga agtgatcaat
     2461 agtacaaccc tgcagctcca cactcctgag ggctatcgac tcaacagaaa tggggactat
     2521 aaggagactc agtactcatt caagcgggta tttggcactc acaccaccca gaaggaactt
     2581 tttgatgttg tagctaatcc cttggtagat gacctcattc atggcaagaa tggtctttta
     2641 tttacatatg gcgtgacggg aagtggaaaa acctacacaa tgacagggtc tccagggtct
     2701 ggaggcctgc ttcctcgttg tttgaatatg atctttaaca gcatagggtc atttcaagca
     2761 aaacgttatg tgtttaagtc taatgatagg aacagtatgg aaatacagtg tgaagtcgat
     2821 gccttgttag aacggcagaa aagagaagcc ttgcccattc caaagacccc ctctagcaag
     2881 cgacaagcag atccagagtt tgcagatatg ataaatgtac aagaattctg caaagcagaa
     2941 gaagttgatg aagacagtgt ctatggagta tttgtctctt acattgaaat ctataataat
     3001 tacatatatg atctattgga agaagtgcag tttgatccca taaagcccaa gtggaacggc
     3061 tgcagtacac ctatgaggaa cgcagagtct gtactcccac aatctaaaac gctccgagaa
     3121 gataagaacc acaatatgta tgtggcagga tgtacagaag tagaagtgaa atctacggag
     3181 gaggcttttg aggttttctg gagagggcag aaaaagagac gcattgctaa cacccatttg
     3241 aatagagagt ccagtcgttc acatagcgtg ttcagcatta aacttgtcca ggctcccctg
     3301 gatgctgatg gagacaatgt cttacaggaa aaagagcaaa ttactataag ccagctgtct
     3361 ctggtagatc ttgctgggag cgaaagaacc aaccgtacta aagcagaagg gaacagacta
     3421 cgtgaggccg gaaacattaa tcagtcattg atgacactaa gaacatgcat ggaggtcctg
     3481 agagagaacc agacgtatgg cactaacaag atggttccat atcgagattc aaagctaacc
     3541 catctattca agaactactt cgatggggag gggaaagttc ggatgatcgt gtgtgtgaat
     3601 ccaaaggctg aagactatga agaaagcttg caagtcatga gatttgctga agtaacccaa
     3661 gaagtggaag tggcaagacc agtggacaag gtgatatgtg gcctgacacc cgggaggcgg
     3721 tacaggaacc tgcctcgggg aggccccgtt ggagatgaac ctttggtgcc tgaagtgatt
     3781 ctacagagct tcccaccgct gcccccatgc aagcttttgg atatcaatga tgaggagacc
     3841 cttccaaagc tggctgacac tttggagaaa cgacatcacc tgcgacaact aatgaccgag
     3901 gaccttaaca aaaaatgtct agctttcaag gccttattaa aagaatttga caattctcta
     3961 tcaaataaag aaaactacgt tcaggaaaaa ctaaatgaaa gagaaaaagt gatctcggga
     4021 cagaaattgg aaatagagcg actggagaag aaaaacaaaa ctttggagta caagattgag
     4081 attctggaga aaacaactac gatctatgag gaagataagc gcaatctgca gcaggagctt
     4141 gagagccaga atcagaagct tcagcggcag ttttctgaca agcgcagatt agaagccagg
     4201 ttgcaaggca tggtaacaga aacgtcgatg aagtggcaga aggaatgtga gcgtcgggtg
     4261 gcagccaccc agctagagat gcagaataaa ctctgggtca aagatgaaaa gctcaaacag
     4321 ctgaaggcca ttgtgactga acccaaacct gagaagccag agagaccctc ccgggagcgg
     4381 gaccgggaga aaatcattcc gagatctgtc tctccttcgc ctctacctct ttctagtaac
     4441 aatattgctc agatttccaa cggccagcaa ctcatgagcc agccgcagct acacagacgc
     4501 tctaactctt gcagcagcat ttctgtagct tcctgtatct cggaatggga gcagaaacta
     4561 tctccattca gcacacctgt caatgtcacc tcccttgcaa ggcataggca gcaggagcca
     4621 ggacaaagta aaacgtgtat cgtgtcagac agaaggcgag gcatgtgctg gactgaaggc
     4681 agggagatgg tccccacatt cagcagtgag ataggcgtag aagaggacca ttgccgcagg
     4741 aacaccccaa ttcctgtacg acacagaagg tcccgctctg cagggagcag atgggtagat
     4801 cataagcctg cctctaatgt gcaaactgag acagtgatgc agccgcatgt ccctcacgcc
     4861 atcacagtgt ctgttgcaaa tgaaaaggcg ctagctaagt gtgagaagta catgctgacc
     4921 caccaggaac tagcctccga tggggagatt cagactaaag tcattaaggg tgatgtttat
     4981 aagacgagag gtggcggaca atcggttcag tttactgata ttgagacttt aaaacaagaa
     5041 ttgccaactg gtagtcggaa acgaagatcg tccaccctag cacctgccca accagatggt
     5101 acagagtctg aatggaccga tgtagaaaca aggtgctctg ttgccgttga aatgagagca
     5161 ggatctcagc tgggaccggg atatcagcac cacgcacaac ccaagcgcaa gaaacctgga
     5221 tccgactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
     5281 gatgacaagt agcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     5341 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     5401 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     5461 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     5521 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     5581 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     5641 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     5701 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     5761 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     5821 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc
     5881 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
     5941 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
     6001 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
     6061 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
     6121 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
     6181 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
     6241 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
     6301 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
     6361 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
     6421 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
     6481 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
     6541 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
     6601 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     6661 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6721 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     6781 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6841 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6901 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6961 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     7021 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     7081 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     7141 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7201 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7261 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7321 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7381 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7441 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7501 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7561 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7621 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     7681 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7741 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7801 ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg
     7861 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7921 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt
     7981 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8041 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8101 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8161 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     8221 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8281 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8341 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     8401 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8461 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt
     8521 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8581 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8641 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8701 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8761 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8821 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8881 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8941 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     9001 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     9061 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9121 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9181 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9241 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9301 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9361 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     9421 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9481 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9541 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9601 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9661 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9721 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9781 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9841 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9901 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9961 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    10021 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    10081 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    10141 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    10201 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    10261 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10321 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10381 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10441 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10501 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10561 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10621 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10681 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    10741 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
    10801 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    10861 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
    10921 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    10981 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
    11041 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
    11101 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
    11161 ccagtgccaa gctg
//
