LOCUS       V037676                10918 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037676
VERSION     V037676
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4944
                     /label="Kpnb1(NM_017063)"
                     /note="Kpnb1(NM_017063)"
                     /gene="Kpnb1"
     CDS             4951..5016
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5060..5271
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5284..5552
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5581..5590
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5611..6273
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6343..6396
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6397..6996
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6997..7585
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7659..7892
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7964..8085
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8125..8260
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8293..8309)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8317..8333
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8341..8371)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8386..8407
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8695..9283)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9454..10314)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10315..10419)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10893..10909
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agctcataac catcctcgag
     2341 aagaccgtgt ctccggatcg gctggagctg gaagcggcgc agaagttcct tgagcgtgcg
     2401 gctgtggaga atctgcccac gttccttgtg gaactgtcca gagtgctggc aaatcccgga
     2461 aacagtcagg ttgccagagt tgcagctggt ctacaaatta agaactcttt gacatcgaag
     2521 gatccagata tcaaggcaca gtaccagcag aggtggcttg ctattgatgc taatgctcgg
     2581 cgggaagtca agaactatgt tttgcagact ttgggcacag agacttaccg gcctagttca
     2641 gcctcacagt gtgtggctgg tatcgcttgt gcagagatcc cagtaagcca gtggccggaa
     2701 ctaattcctc agctggtagc caatgtcacc aaccccaaca gcacggagca catgaaagag
     2761 tccacactgg aagccatcgg ttacatttgc caagatatag acccggagca gctgcaggat
     2821 aaatccaatg agatcctgac tgccataatc cagggcatgc ggaaggaaga gcctagtaac
     2881 aatgtgaagc tggccgctac caacgcgctc ctgaactcac tggagttcac caaagcaaac
     2941 ttcgacaaag agtctgaacg gcactttatc atgcaagtgg tctgtgaagc cacacagtgt
     3001 ccagatacaa gggtaagagt ggctgcttta cagaatctag tgaagataat gtccttgtat
     3061 taccagtaca tggagacata catgggccct gccctctttg caatcacaat tgaagcaatg
     3121 aaaagtgaca ttgatgaggt agctctccaa gggatagagt tctggtccaa tgtctgtgat
     3181 gaggaaatgg atttggccat tgaggcttca gaggcagcag aacaaggacg gcccccggag
     3241 cacaccagca aattttacgc caagggagcg ctgcagtacc tggtgcccat cctcacacag
     3301 acactaacta aacaggatga aaacgatgac gacgatgact ggaacccttg caaagcagct
     3361 ggggtgtgcc tcatgctcct gtctacctgc tgtgaagatg acattgtgcc acatgtcctt
     3421 ccctttatta aagagcacat caagaaccct gactggcggt accgggatgc agcagtgatg
     3481 gcttttggca gcatcttgga aggaccagag cctaaccagc ttaaaccatt agtcatacag
     3541 gctatgccca ccctgataga attaatgaaa gaccccagtg tagttgttcg agatacaaca
     3601 gcatggactg tgggcaggat ctgtgagctg ctgcctgaag ctgccatcaa tgatgtctac
     3661 ttggcacccc ttttacagtg tctgattgag ggtctcagtg ctgaacccag agtggcttca
     3721 aatgtgtgct gggctttttc cagtctggct gaagctgctt acgaagctgc agatgtagct
     3781 gatgatcagg aagaaccagc cacctattgc ttgtcttctt cctttgaact tatagttcag
     3841 aagctgctgg agaccaccga cagacctgat ggacaccaga acaacctgag aagttctgca
     3901 tatgaatctc taatggaaat tgtaaaaaac agtgccaagg attgttaccc tgcggtgcag
     3961 aagaccaccc tggtcattat ggaacggttg cagcaggtgc ttcagatgga gtcccacatc
     4021 cagagcacgt cggacaggat ccaattcaat gacctccagt ctctactctg tgcaactctt
     4081 cagaatgttc tccggaaagt gcagcatcaa gatgctttgc agatctctga tgtggtcatg
     4141 gcctccctgt taaggatgtt ccaaagcaca gctgggtctg gcggagtgca agaagacgcc
     4201 ctgatggcag tcagcacact ggtggaagtg ttgggtggtg aattcctcaa gtacatggag
     4261 gcctttaaac cattcctggg cattggattg aaaaattatg ctgaatacca ggtatgtttg
     4321 gcagctgttg gcttagttgg agacttgtgc cgagccctgc agtctaacat cttacctttc
     4381 tgtgatgagg tgatgcagct gctcctagag aacttgggga atgagaatgt ccacaggtct
     4441 gtgaaaccac agattctgtc tgtgtttggt gacattgctc ttgccattgg tggagaattt
     4501 aagaaatact tggaggttgt actgaataca ctgcagcagg cctcccaagc ccaagtggac
     4561 aagtcagact ttgacatggt ggattatctg aatgagctaa gagaaagctg cttggaagct
     4621 tatacgggaa tcgtccaggg attgaaggga gaccaggaaa acgtacaccc tgatgtaatg
     4681 ctggtgcaac ccagagtaga atttattttg tcttttattg atcacattgc tggagatgag
     4741 gatcatacgg atggagtggt agcttgtgct gctggactga taggggactt gtgtacagca
     4801 tttggaaagg atgtactgaa attagtagaa gctaggccaa tgatccatga actattaact
     4861 gaagggcgga gatcgaagac taacaaagca aagacccttg ctacgtgggc aacaaaggaa
     4921 ctgaggaaac tgaagaacca agctggatcc gactacaaag accatgacgg tgattataaa
     4981 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     5041 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     5101 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     5161 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     5221 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     5281 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     5341 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     5401 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     5461 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     5521 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     5581 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     5641 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     5701 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     5761 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     5821 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     5881 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     5941 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     6001 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     6061 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     6121 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     6181 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     6241 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     6301 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     6361 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     6421 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     6481 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     6541 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     6601 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     6661 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     6721 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6781 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6841 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6901 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     6961 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     7021 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7081 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7141 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7201 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7261 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7321 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7381 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7441 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7501 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7561 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     7621 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7681 acgaagataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7741 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7801 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7861 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7921 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7981 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8041 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     8101 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8161 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8221 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa
     8281 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8341 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8401 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8461 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8521 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8581 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8641 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8701 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8761 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8821 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8881 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8941 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9001 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9061 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9121 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9181 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9241 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9301 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9361 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9421 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9481 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9541 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9601 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9661 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9721 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9781 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9841 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9901 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9961 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10021 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10081 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10141 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10201 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10261 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10321 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10381 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10441 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    10501 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    10561 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    10621 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
    10681 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    10741 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    10801 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    10861 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
