LOCUS       V033755                10358 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033755
VERSION     V033755
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5190
                     /label="LRP8(NM_004631)"
                     /note="LRP8(NM_004631)"
                     /gene="LRP8"
     CDS             5197..5223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5227..5253
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5257..5283
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        5328..5539
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5552..5820
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5847..6446
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6453..7041
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7115..7348
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7420..7541
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7560..7695
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7733..7749)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7757..7773
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7781..7811)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7826..7847
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8135..8723)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8894..9754)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9755..9859)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10333..10349
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgggcctc cccgagccgg gccctctccg gcttctggcg
     2341 ctgctgctgc tgctgctgct gctgctgctg ctgcagctcc agcatcttgc ggcggcagcg
     2401 gctgatccgc tgctcggcgg ccaagggccg gccaaggatt gcgaaaagga ccaattccag
     2461 tgccggaacg agcgctgcat cccctctgtg tggagatgcg acgaggacga tgactgctta
     2521 gaccacagcg acgaggacga ctgccccaag aagacctgtg cagacagtga cttcacctgt
     2581 gacaacggcc actgcatcca cgaacggtgg aagtgtgacg gcgaggagga gtgtcctgat
     2641 ggctccgatg agtccgaggc cacttgcacc aagcaggtgt gtcctgcaga gaagctgagc
     2701 tgtggaccca ccagccacaa gtgtgtacct gcctcgtggc gctgcgacgg ggagaaggac
     2761 tgcgagggtg gagcggatga ggccggctgt gctaccttgt gcgccccgca cgagttccag
     2821 tgcggcaacc gctcgtgcct ggccgccgtg ttcgtgtgcg acggcgacga cgactgtggt
     2881 gacggcagcg atgagcgcgg ctgtgcagac ccggcctgcg ggccccgcga gttccgctgc
     2941 ggcggcgatg gcggcggcgc ctgcatcccg gagcgctggg tctgcgaccg ccagtttgac
     3001 tgcgaggacc gctcggacga ggcagccgag ctctgcggcc gtccgggccc cggggccacg
     3061 tccgcgcccg ccgcctgcgc caccgcctcc cagttcgcct gccgcagcgg cgagtgcgtg
     3121 cacctgggct ggcgctgcga cggcgaccgc gactgcaaag acaaatcgga cgaggccgac
     3181 tgcccactgg gcacctgccg tggggacgag ttccagtgtg gggatgggac atgtgtcctt
     3241 gcaatcaagc actgcaacca ggagcaggac tgtccagatg ggagtgatga agctggctgc
     3301 ctacaggggc tgaacgagtg tctgcacaac aatggcggct gctcacacat ctgcactgac
     3361 ctcaagattg gctttgaatg cacgtgccca gcaggcttcc agctcctgga ccagaagacc
     3421 tgtggcgaca ttgatgagtg caaggaccca gatgcctgca gccagatctg tgtcaattac
     3481 aagggctatt ttaagtgtga gtgctaccct ggctacgaga tggacctact gaccaagaac
     3541 tgcaaggctg ctgctggcaa gagcccatcc ctaatcttca ccaaccggca cgaggtgcgg
     3601 aggatcgacc tggtgaagcg gaactattca cgcctcatcc ccatgctcaa gaatgtcgtg
     3661 gcactagatg tggaagttgc caccaatcgc atctactggt gtgacctctc ctaccgtaag
     3721 atctatagcg cctacatgga caaggccagt gacccgaaag agcaggaggt cctcattgac
     3781 gagcagttgc actctccaga gggcctggca gtggactggg tccacaagca catctactgg
     3841 actgactcgg gcaataagac catctcagtg gccacagttg atggtggccg ccgacgcact
     3901 ctcttcagcc gtaacctcag tgaaccccgg gccatcgctg ttgaccccct gcgagggttc
     3961 atgtattggt ctgactgggg ggaccaggcc aagattgaga aatctgggct caacggtgtg
     4021 gaccggcaaa cactggtgtc agacaatatt gaatggccca acggaatcac cctggatctg
     4081 ctgagccagc gcttgtactg ggtagactcc aagctacacc aactgtccag cattgacttc
     4141 agtggaggca acagaaagac gctgatctcc tccactgact tcctgagcca cccttttggg
     4201 atagctgtgt ttgaggacaa ggtgttctgg acagacctgg agaacgaggc cattttcagt
     4261 gcaaatcggc tcaatggcct ggaaatctcc atcctggctg agaacctcaa caacccacat
     4321 gacattgtca tcttccatga gctgaagcag ccaagagctc cagatgcctg tgagctgagt
     4381 gtccagccta atggaggctg tgaatacctg tgccttcctg ctcctcagat ctccagccac
     4441 tctcccaagt acacatgtgc ctgtcctgac acaatgtggc tgggtccaga catgaagagg
     4501 tgctaccgag cacctcaatc tacctcaact acgacgttag cttctaccat gacgaggaca
     4561 gtacctgcca ccacaagagc ccccgggacc accgtccaca gatccaccta ccagaaccac
     4621 agcacagaga caccaagcct gacagctgca gtcccaagct cagttagtgt ccccagggct
     4681 cccagcatca gcccgtctac cctaagccct gcaaccagca accactccca gcactatgca
     4741 aatgaagaca gtaagatggg ctcaacagtc actgccgctg ttatcgggat catcgtgccc
     4801 atagtggtga tagccctcct gtgcatgagt ggatacctga tctggagaaa ctggaagcgg
     4861 aagaacacca aaagcatgaa ttttgacaac ccagtctaca ggaaaacaac agaagaagaa
     4921 gacgaagatg agctccatat agggagaact gctcagattg gccatgtcta tcctgcagca
     4981 atcagcagct ttgatcgccc actgtgggca gagccctgtc ttggggagac cagagaaccg
     5041 gaagacccag cccctgccct caaggagctt tttgtcttgc cgggggaacc aaggtcacag
     5101 ctgcaccaac tcccgaagaa ccctctttcc gagctgcctg tcgtcaaatc caagcgagtg
     5161 gcattaagcc ttgaagatga tggactaccc ggatcctacc catacgacgt acctgattat
     5221 gcaacttatc cttatgatgt acctgattat gctagctatc cttatgatgt cccagactac
     5281 gcataggcgg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac
     5341 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag
     5401 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     5461 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     5521 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg
     5581 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     5641 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     5701 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     5761 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     5821 caagctgtga ccggcgccta cgctagatga ccgagtacaa gcccacggtg cgcctcgcca
     5881 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     5941 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     6001 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     6061 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     6121 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     6181 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     6241 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     6301 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     6361 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     6421 gcatgacccg caagcccggt gcctgagtcg acaatcaacc tctggattac aaaatttgtg
     6481 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6541 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6601 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6661 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6721 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6781 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6841 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6901 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6961 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7021 ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta
     7081 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7141 aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7201 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7261 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7321 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7381 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     7441 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7501 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     7561 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7621 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7681 gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc
     7741 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7801 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7861 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7921 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7981 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8041 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8101 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8161 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8221 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8281 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8341 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8401 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8461 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8521 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8581 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8641 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8701 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8761 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8821 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8881 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8941 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9001 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9061 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9121 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9181 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9241 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9301 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9361 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9421 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9481 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9541 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9601 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9661 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9721 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9781 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9841 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     9901 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     9961 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    10021 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    10081 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
    10141 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
    10201 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    10261 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
    10321 agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
