LOCUS       V032201                11568 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032201
VERSION     V032201
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..5650
                     /label="MECOM(NM_001105077)"
                     /note="MECOM(NM_001105077)"
                     /gene="MECOM"
     CDS             5657..5722
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5766..5977
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5990..6258
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6293..7000
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             7010..7063
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7064..7663
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7664..8251
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8325..8558
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8630..8751
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8770..8905
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8943..8959)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8967..8983
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8991..9021)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9036..9057
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9345..9933)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10104..10964)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10965..11069)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11543..11559
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgatctt agacgaattt tacaatgtga agttctgcat
     2341 agatgccagt caaccagatg ttggaagctg gctcaagtac attagattcg ctggctgtta
     2401 tgatcagcac aaccttgttg catgccagat aaatgatcag atattctata gagtagttgc
     2461 agacattgcg ccgggagagg agcttctgct gttcatgaag agcgaagact atccccatga
     2521 aactatggcg ccggatatcc acgaagaacg gcaatatcgc tgcgaagact gtgaccagct
     2581 ctttgaatct aaggctgaac tagcagatca ccaaaagttt ccatgcagta ctcctcactc
     2641 agcattttca atggttgaag aggactttca gcaaaaactc gaaagcgaga atgatctcca
     2701 agagatacac acgatccagg agtgtaagga atgtgaccaa gtttttcctg atttgcaaag
     2761 cctggagaaa cacatgctgt cacatactga agagagggaa tacaagtgtg atcagtgtcc
     2821 caaggcattt aactggaagt ccaatttaat tcgccaccag atgtcacatg acagtggaaa
     2881 gcactatgaa tgtgaaaact gtgccaagca ggttttcacg gaccctagca accttcagcg
     2941 gcacattcgc tctcagcatg tcggtgcccg ggcccatgca tgcccggagt gtggcaaaac
     3001 gtttgccact tcgtcgggcc tcaaacaaca caagcacatc cacagcagtg tgaagccctt
     3061 tatctgtgag gtctgccata aatcctatac tcagttttca aacctttgcc gtcataagcg
     3121 catgcatgct gattgcagaa cccaaatcaa gtgcaaagac tgtggacaaa tgttcagcac
     3181 tacgtcttcc ttaaataaac acaggaggtt ttgtgagggc aagaaccatt ttgcggcagg
     3241 tggatttttt ggccaaggca tttcacttcc tggaacccca gctatggata aaacgtccat
     3301 ggttaatatg agtcatgcca acccgggcct tgctgactat tttggcgcca ataggcatcc
     3361 tgctggtctt acctttccaa cagctcctgg attttctttt agcttccctg gtctgtttcc
     3421 ttccggcttg taccacaggc ctcctttgat acctgctagt tctcctgtta aaggactatc
     3481 aagtactgaa cagacaaaca aaagtcaaag tcccctcatg acacatcctc agatactgcc
     3541 agctacacag gatattttga aggcactatc taaacaccca tctgtagggg acaataagcc
     3601 agtggagctc cagcccgaga ggtcctctga agagaggccc tttgagaaaa tcagtgacca
     3661 gtcagagagt agtgaccttg atgatgtcag tacaccaagt ggcagtgacc tggaaacaac
     3721 ctcgggctct gatctggaaa gtgacattga aagtgataaa gagaaattta aagaaaatgg
     3781 taaaatgttc aaagacaaag taagccctct tcagaatctg gcttcaataa ataataagaa
     3841 agaatacagc aatcattcca ttttctcacc atctttagag gagcagactg cggtgtcagg
     3901 agctgtgaat gattctataa aggctattgc ttctattgct gaaaaatact ttggttcaac
     3961 aggactggtg gggctgcaag acaaaaaagt tggagcttta ccttaccctt ccatgtttcc
     4021 cctcccattt tttccagcat tctctcaatc aatgtaccca tttcctgata gagacttgag
     4081 atcgttacct ttgaaaatgg aaccccaatc accaggtgaa gtaaagaaac tgcagaaggg
     4141 cagctctgag tccccctttg atctcaccac taagcgaaag gatgagaagc ccttgactcc
     4201 agtcccctcc aagcctccag tgacacctgc cacaagccaa gaccagcccc tggatctaag
     4261 tatgggcagt aggagtagag ccagtgggac aaagctgact gagcctcgaa aaaaccacgt
     4321 gtttggggga aaaaaaggaa gcaacgtcga atcaagacct gcttcagatg gttccttgca
     4381 gcatgcaaga cccactcctt tctttatgga ccctatttac agagtagaga aaagaaaact
     4441 aactgaccca cttgaagctt taaaagagaa atacttgagg ccttctccag gattcttgtt
     4501 tcacccacaa ttccaactgc ctgatcagag aacttggatg tcagctattg aaaacatggc
     4561 agaaaagcta gagagcttca gtgccctgaa acctgaggcc agtgagctct tacagtcagt
     4621 gccctctatg ttcaacttca gggcgcctcc caatgccctg ccagagaacc ttctgcggaa
     4681 gggaaaggag cgctatacct gcagatactg tggcaagatt tttccaaggt ctgcaaacct
     4741 aacacggcac ttgagaaccc acacaggaga gcagccttac agatgcaaat actgtgacag
     4801 atcatttagc atatcttcta acttgcaaag gcatgttcgc aacatccaca ataaagagaa
     4861 gccatttaag tgtcacttat gtgataggtg ttttggtcaa caaaccaatt tagacagaca
     4921 cctaaagaaa catgagaatg ggaacatgtc cggtacagca acatcgtcgc ctcattctga
     4981 actggaaagt acaggtgcga ttctggatga caaagaagat gcttacttca cagaaattcg
     5041 aaatttcatt gggaacagca accatggcag ccaatctccc aggaatgtgg aggagagaat
     5101 gaatggcagt cattttaaag atgaaaaggc tttggtgacc agtcaaaatt cagacttgct
     5161 ggatgatgaa gaagttgaag atgaggtgtt gttagatgag gaggatgaag acaatgatat
     5221 tactggaaaa acaggaaagg aaccagtgac aagtaattta catgaaggaa accctgagga
     5281 tgactatgaa gaaaccagtg ccctggagat gagttgcaag acatccccag tgaggtataa
     5341 agaggaagaa tataaaagtg gactttctgc tctagatcat ataaggcact tcacagatag
     5401 cctcaaaatg aggaaaatgg aagataatca atattctgaa gctgagctgt cttcttttag
     5461 tacttcccat gtgccagagg aacttaagca gccgttacac agaaagtcca aatcgcaggc
     5521 atatgctatg atgctgtcac tgtctgacaa ggagtccctc cattctacat cccacagttc
     5581 ttccaacgtg tggcacagta tggccagggc tgcggcggaa tccagtgcta tccagtccat
     5641 aagccacgta ggatccgact acaaagacca tgacggtgat tataaagatc atgacatcga
     5701 ctacaaggat gacgatgaca agtagcggcc gcgaaggatc tgcgatcgct ccggtgcccg
     5761 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     5821 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     5881 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     5941 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
     6001 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
     6061 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
     6121 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
     6181 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
     6241 tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggtgag
     6301 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     6361 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     6421 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     6481 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     6541 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     6601 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     6661 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     6721 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     6781 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     6841 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     6901 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     6961 gggccgccac tccaccggcg gcatggacga gctgtacaag ggatctcgcg agggcagagg
     7021 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc
     7081 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     7141 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     7201 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     7261 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     7321 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     7381 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     7441 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     7501 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     7561 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     7621 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta
     7681 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7741 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7801 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7861 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7921 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7981 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     8041 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     8101 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     8161 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt ctccgccttc gccctcagac
     8221 gagtcggatc tccctttggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     8281 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     8341 ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8401 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8461 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8521 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8581 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     8641 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8701 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8761 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8821 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8881 tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca
     8941 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9001 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9061 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9121 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9181 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9241 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9301 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9361 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9421 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9481 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9541 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9601 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9661 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9721 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9781 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9841 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9901 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9961 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10021 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10081 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10141 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10201 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10261 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10321 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10381 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10441 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10501 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10561 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10621 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10681 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10741 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10801 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10861 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10921 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10981 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11041 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    11101 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    11161 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
    11221 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
    11281 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
    11341 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
    11401 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    11461 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
    11521 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
