LOCUS       V038164                12652 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038164
VERSION     V038164
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..6678
                     /label="MED14(NM_004229)"
                     /note="MED14(NM_004229)"
                     /gene="MED14"
     CDS             6685..6750
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6794..7005
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7018..7286
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      7315..7324
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             7345..8007
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8077..8130
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8131..8730
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8731..9319
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9393..9626
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9698..9819
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9859..9994
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10027..10043)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10051..10067
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10075..10105)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10120..10141
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10429..11017)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11188..12048)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12049..12153)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12627..12643
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ccccagtgca gctggagaac
     2341 caccagctgg tcccgcccgg aggcggcggc gggggcagcg gcggaccccc gtcagcccca
     2401 gcccctcctc ccccgggagc cgccgtggcg gcggccgctg cagctgcggc tagcccgggc
     2461 taccggctga gcaccctcat tgaatttctg ctgcaccggg cctactcgga gcttatggtg
     2521 ttgacggacc tactgccaag gaaatctgat gtggaaagga aaatagaaat agtgcagttt
     2581 gctagccgga cacgccaact cttcgttcga ttattagctt tagtgaaatg ggctaataat
     2641 gctggcaaag tggaaaaatg tgcgatgatt tcaagctttt tagatcagca agccatcctg
     2701 tttgtggaca ctgctgatcg cctggcctcg ttagctagag atgctctggt ccatgcacgc
     2761 ctgcctagtt ttgccatccc atatgccatt gatgtactaa ctactggatc ttacccacgg
     2821 ctgccaacct gcattaggga caaaattatt cctccagacc caattaccaa aattgaaaaa
     2881 caagccacac tccatcagct gaatcagatt cttagacatc ggcttgtaac cacagatctt
     2941 cctcctcagt tagcaaatct tacagttgca aatggccggg tgaagtttcg tgttgaagga
     3001 gaatttgaag ccaccttgac tgtgatggga gatgaccctg atgttccatg gcgtcttctc
     3061 aagctagaaa ttctagttga ggataaggaa acaggagatg ggcgagcttt ggttcatagc
     3121 atgcaaatca gcttcatcca tcaactggtg cagtctaggc tctttgctga tgagaaacct
     3181 cttcaggata tgtacaactg cctacattct ttctgtttat cacttcagtt agaagtgtta
     3241 cattcccaaa ctctaatgtt aatccgagaa cggtggggag accttgtgca ggtggaaagg
     3301 tatcatgctg gaaagtgcct ctccctttca gtttggaatc aacaggttct tgggagaaaa
     3361 actggaacag catctgttca caaagttaca attaaaattg atgagaatga tgtctccaag
     3421 cctttacaga tttttcacga tcctcctttg ccagcttctg attccaaatt agtagaaaga
     3481 gccatgaaga tcgaccactt atcaatagaa aaactcctga ttgacagtgt ccatgcaaga
     3541 gctcatcaga agctccaaga actgaaggcc attcttagag gcttcaatgc caatgaaaac
     3601 tcttccatag agactgcact cccagctctt gttgttccca tcttggagcc ctgtggtaat
     3661 tcagagtgtc tgcacatttt tgtagattta cattctggaa tgtttcaatt gatgctttat
     3721 ggacttgacc aggccactct ggatgacatg gagaagtctg tgaatgatga tatgaaacga
     3781 atcataccct ggattcagca acttaagttt tggcttggac aacagcgttg caagcagtct
     3841 ataaaacatc tgcctacgat aagcagtgaa acattgcagc tttccaatta ctcaactcat
     3901 cctattggaa acctttctaa gaataaactg ttcattaaac ttacccgcct tccacaatac
     3961 tacattgttg tggagatgtt ggaggttccc aataaaccca cacaactgtc gtacaagtac
     4021 tactttatgt ctgtgaatgc tgcagatcgt gaagacagcc ctgcaatggc attgctgctg
     4081 cagcagttca aggaaaacat tcaggacttg gtttttcgta caaaaaccgg gaaacagacc
     4141 agaaccaatg ccaagcgcaa gttgtctgat gatccatgtc cagtagaatc caagaaaaca
     4201 aaacgagcag gagaaatgtg tgccttcaat aaagttttag cccacttcgt cgctatgtgt
     4261 gatacaaata tgccatttgt aggacttcgg ttggagttgt ccaatctgga gattccacat
     4321 caaggagtgc aagtggaagg tgatggcttc agccatgcaa ttcgcttatt aaaaattcct
     4381 ccctgtaagg gtataactga ggaaacccaa aaggctctgg accgctctct tcttgattgc
     4441 actttccgat tacaaggtag aaataaccgc acttgggtag cagagttagt gtttgcaaat
     4501 tgtccactta atggcacttc tactagggag caaggaccat cccggcacgt ttacctgaca
     4561 tatgaaaatc tgttgtctga gcctgttggt ggtagaaagg tggttgaaat gtttcttaat
     4621 gactggaata gcattgcacg attatatgag tgtgtgttgg aatttgcacg ttctctacca
     4681 gacatacctg ctcatctaaa tattttctca gaagttcgtg tttataatta ccgaaaactt
     4741 atcttgtgtt atggaaccac caagggaagc tcaattagta tccaatggaa ttcgatccat
     4801 caaaaattcc acatttcttt gggaactgtt ggcccaaact caggttgcag taactgtcac
     4861 aataccattc tccatcagct tcaagaaatg ttcaacaaaa caccaaatgt ggttcagtta
     4921 ttacaggtac tgtttgatac tcaggctcca ttaaatgcca tcaacaaact ccccactgtg
     4981 ccgatgttgg gcttgaccca gagaaccaat actgcctacc agtgcttctc cattctgcca
     5041 cagtcgtcca cccacatcag actggccttc aggaacatgt attgcattga tatatactgc
     5101 cggagtcgag gtgttgtggc aatacgggat ggtgcctata gtctttttga taacagcaaa
     5161 ctagttgaag gtttctatcc tgcaccagga ctaaagacgt tcctgaatat gtttgttgac
     5221 agcaatcagg atgctcgaag aaggtctgta aatgaggacg ataatccccc ttctcctata
     5281 ggaggagata tgatggattc tttaatatcg cagctccagc caccacccca gcaacagcca
     5341 tttccaaagc agccaggaac atcaggtgct tatcctctta cttcaccccc tacatcttat
     5401 catagcacag tcaatcagtc tccctcaatg atgcacacac agtctccagg aaatctgcat
     5461 gctgccagct cccccagtgg ggctttgaga gccccatcac cagcgtcatt tgttccaact
     5521 cctcccccat cctcgcatgg aatctcaata ggaccagggg ccagttttgc tagtccacat
     5581 ggaactcttg accctagttc cccatacact atggtgtcac caagtggacg agcagggaac
     5641 tggccaggat ctcctcaagt gtctggcccc tcaccagcag cacgcatgcc tggaatgtca
     5701 ccagccaacc cctcactaca ttctccggtt ccagatgctt ctcattcccc tcgagctgga
     5761 acaagttctc aaacaatgcc aacaaacatg cctccacctc gtaaactacc tcagcgctct
     5821 tgggcggcat ccatacctac catcctcact cacagtgcct tgaacatttt actgctgccc
     5881 tctccaactc caggccttgt gcccggcctg gcaggtagtt acctttgttc tccacttgag
     5941 agattccttg gatcagtcat catgagacga catcttcaaa gaattattca acaagaaacg
     6001 ctgcagctga taaattctaa tgaacccgga gtgatcatgt ttaagactga tgcactgaaa
     6061 tgcagagtag ctcttagtcc caaaaccaac caaacgcttc agctaaaagt gacacctgaa
     6121 aatgcaggac agtggaaacc tgatgaactt caagttttgg agaaattctt tgaaacaaga
     6181 gttgcaggac caccatttaa agctaatacg ttaatagcct tcaccaagct attaggagct
     6241 cctacacaca tcctcaggga ttgtgtgcac attatgaagc tggagctgtt ccctgaccaa
     6301 gcaacacagc taaaatggaa tgttcagttt tgcctgacga tccctcccag cgcgccgccg
     6361 attgcacctc ctgggacgcc tgctgtggtg ctgaaatcca aaatgctatt ttttcttcaa
     6421 ctaactcaga aaacatcggt ccctccccaa gaacctgtta gtattatagt tccaatcatt
     6481 tatgacatgg cttcaggtac aacccagcag gcagacattc ccagacagca gaactcttct
     6541 gttgctgctc ccatgatggt cagcaacatt ctgaagaggt ttgcagagat gaatccacca
     6601 cgacaaggtg aatgcacaat atttgcagct gttcgtgatt taatggctaa tcttacactg
     6661 ccccctggtg ggcgtccagg atccgactac aaagaccatg acggtgatta taaagatcat
     6721 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     6781 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     6841 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     6901 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     6961 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     7021 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     7081 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     7141 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     7201 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     7261 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc
     7321 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc
     7381 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc
     7441 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc
     7501 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc
     7561 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac
     7621 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac
     7681 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc
     7741 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc
     7801 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac
     7861 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc
     7921 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac
     7981 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg
     8041 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca
     8101 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc
     8161 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     8221 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     8281 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     8341 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     8401 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     8461 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     8521 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     8581 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     8641 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     8701 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa
     8761 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     8821 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8881 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8941 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     9001 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     9061 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     9121 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     9181 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     9241 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     9301 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     9361 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     9421 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     9481 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9541 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9601 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     9661 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     9721 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9781 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     9841 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9901 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9961 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
    10021 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    10081 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    10141 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    10201 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    10261 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    10321 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    10381 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    10561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10621 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10861 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    11041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    11101 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    11161 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    11221 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    11281 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    11341 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11401 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11461 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11521 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11581 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    11641 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    11701 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    11761 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11821 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11881 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11941 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    12001 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    12061 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    12121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    12181 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    12241 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    12301 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    12361 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    12421 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    12481 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    12541 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    12601 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
