LOCUS       V044793                11246 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044793
VERSION     V044793
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      2311..2320
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             2320..2343
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             2344..5340
                     /label="MERTK(NM_006343)"
                     /note="MERTK(NM_006343)"
                     /gene="MERTK"
     promoter        5405..5616
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5629..5897
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5926..5935
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5956..6618
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6688..6741
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6742..7341
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7342..7929
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8003..8236
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8308..8429
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8448..8583
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8621..8637)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8645..8661
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8669..8699)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8714..8735
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9023..9611)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9782..10642)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10643..10747)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11221..11237
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg actacaagga cgacgatgac
     2341 aagatggggc cggccccgct gccgctgctg ctgggcctct tcctccccgc gctctggcgt
     2401 agagctatca ctgaggcaag ggaagaagcc aagccttacc cgctattccc gggacctttt
     2461 ccagggagcc tgcaaactga ccacacaccg ctgttatccc ttcctcacgc cagtgggtac
     2521 cagcctgcct tgatgttttc accaacccag cctggaagac cacatacagg aaacgtagcc
     2581 attccccagg tgacctctgt cgaatcaaag cccctaccgc ctcttgcctt caaacacaca
     2641 gttggacaca taatactttc tgaacataaa ggtgtcaaat ttaattgctc aatcagtgta
     2701 cctaatatat accaggacac cacaatttct tggtggaaag atgggaagga attgcttggg
     2761 gcacatcatg caattacaca gttttatcca gatgatgaag ttacagcaat aatcgcttcc
     2821 ttcagcataa ccagtgtgca gcgttcagac aatgggtcgt atatctgtaa gatgaaaata
     2881 aacaatgaag agatcgtgtc tgatcccatc tacatcgaag tacaaggact tcctcacttt
     2941 actaagcagc ctgagagcat gaatgtcacc agaaacacag ccttcaacct cacctgtcag
     3001 gctgtgggcc cgcctgagcc cgtcaacatt ttctgggttc aaaacagtag ccgtgttaac
     3061 gaacagcctg aaaaatcccc ctccgtgcta actgttccag gcctgacgga gatggcggtc
     3121 ttcagttgtg aggcccacaa tgacaaaggg ctgaccgtgt ccaagggagt gcagatcaac
     3181 atcaaagcaa ttccctcccc accaactgaa gtcagcatcc gtaacagcac tgcacacagc
     3241 attctgatct cctgggttcc tggttttgat ggatactccc cgttcaggaa ttgcagcatt
     3301 caggtcaagg aagctgatcc gctgagtaat ggctcagtca tgatttttaa cacctctgcc
     3361 ttaccacatc tgtaccaaat caagcagctg caagccctgg ctaattacag cattggtgtt
     3421 tcctgcatga atgaaatagg ctggtctgca gtgagccctt ggattctagc cagcacgact
     3481 gaaggagccc catcagtagc acctttaaat gtcactgtgt ttctgaatga atctagtgat
     3541 aatgtggaca tcagatggat gaagcctccg actaagcagc aggatggaga actggtgggc
     3601 taccggatat cccacgtgtg gcagagtgca gggatttcca aagagctctt ggaggaagtt
     3661 ggccagaatg gcagccgagc tcggatctct gttcaagtcc acaatgctac gtgcacagtg
     3721 aggattgcag ccgtcaccag agggggagtt gggcccttca gtgatccagt gaaaatattt
     3781 atccctgcac acggttgggt agattatgcc ccctcttcaa ctccggcgcc tggcaacgca
     3841 gatcctgtgc tcatcatctt tggctgcttt tgtggattta ttttgattgg gttgatttta
     3901 tacatctcct tggccatcag aaaaagagtc caggagacaa agtttgggaa tgcattcaca
     3961 gaggaggatt ctgaattagt ggtgaattat atagcaaaga aatccttctg tcggcgagcc
     4021 attgaactta ccttacatag cttgggagtc agtgaggaac tacaaaataa actagaagat
     4081 gttgtgattg acaggaatct tctaattctt ggaaaaattc tgggtgaagg agagtttggg
     4141 tctgtaatgg aaggaaatct taagcaggaa gatgggacct ctctgaaagt ggcagtgaag
     4201 accatgaagt tggacaactc ttcacagcgg gagatcgagg agtttctcag tgaggcagcg
     4261 tgcatgaaag acttcagcca cccaaatgtc attcgacttc taggtgtgtg tatagaaatg
     4321 agctctcaag gcatcccaaa gcccatggta attttaccct tcatgaaata cggggacctg
     4381 catacttact tactttattc ccgattggag acaggaccaa agcatattcc tctgcagaca
     4441 ctattgaagt tcatggtgga tattgccctg ggaatggagt atctgagcaa caggaatttt
     4501 cttcatcgag atttagctgc tcgaaactgc atgttgcgag atgacatgac tgtctgtgtt
     4561 gcggacttcg gcctctctaa gaagatttac agtggcgatt attaccgcca aggccgcatt
     4621 gctaagatgc ctgttaaatg gatcgccata gaaagtcttg cagaccgagt ctacacaagt
     4681 aaaagtgatg tgtgggcatt tggcgtgacc atgtgggaaa tagctacgcg gggaatgact
     4741 ccctatcctg gggtccagaa ccatgagatg tatgactatc ttctccatgg ccacaggttg
     4801 aagcagcccg aagactgcct ggatgaactg tatgaaataa tgtactcttg ctggagaacc
     4861 gatcccttag accgccccac cttttcagta ttgaggctgc agctagaaaa actcttagaa
     4921 agtttgcctg acgttcggaa ccaagcagac gttatttacg tcaatacaca gttgctggag
     4981 agctctgagg gcctggccca gggctccacc cttgctccac tggacttgaa catcgaccct
     5041 gactctataa ttgcctcctg cactccccgc gctgccatca gtgtggtcac agcagaagtt
     5101 catgacagca aacctcatga aggacggtac atcctgaatg ggggcagtga ggaatgggaa
     5161 gatctgactt ctgccccctc tgctgcagtc acagctgaaa agaacagtgt tttaccgggg
     5221 gagagacttg ttaggaatgg ggtctcctgg tcccattcga gcatgctgcc cttgggaagc
     5281 tcattgcccg atgaactttt gtttgctgac gactcctcag aaggctcaga agtcctgatg
     5341 tgaggatccc tgcgatatcc agcgcggccg cgaaggatct gcgatcgctc cggtgcccgt
     5401 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5461 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5521 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5581 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     5641 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     5701 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     5761 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     5821 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     5881 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catggagagc
     5941 gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc
     6001 gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac
     6061 aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg
     6121 ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac
     6181 gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg
     6241 ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg
     6301 gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac
     6361 gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc
     6421 accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc
     6481 aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc
     6541 gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag
     6601 acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt ggacggcacc
     6661 gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac atgcggtgac
     6721 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     6781 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6841 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6901 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6961 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     7021 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7081 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7141 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7201 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     7261 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7321 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     7381 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7441 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7501 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7561 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7621 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7681 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     7741 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7801 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7861 ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttggccg
     7921 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7981 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa ataagatctg
     8041 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8101 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8161 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8221 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     8281 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     8341 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8401 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     8461 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8521 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8581 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt
     8641 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8701 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8761 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     8821 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8881 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8941 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9001 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9061 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9121 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9181 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9241 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9301 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9361 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9421 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     9481 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9541 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9601 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9661 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9721 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9781 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9841 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9901 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9961 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10021 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10081 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10141 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10201 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10261 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10321 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10381 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10441 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10501 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10561 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10621 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10681 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10741 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
    10801 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
    10861 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
    10921 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
    10981 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
    11041 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
    11101 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
    11161 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
    11221 gtaaaacgac ggccagtgcc aagctg
//
