LOCUS V020383 8518 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020383 VERSION V020383 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1798..1915 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 2005..2208 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 2289..3437 /label="MICA(NM_000247)" /note="MICA(NM_000247)" /gene="MICA" promoter 3488..3699 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 3712..3980 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 4007..4606 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 4613..5201 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 5275..5508 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 5580..5701 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 5720..5855 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(5893..5909) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5917..5933 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5941..5971) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5986..6007 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6295..6883) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7054..7914) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7915..8019) /gene="bla" /label="AmpR promoter" primer_bind 8493..8509 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagccaccat ggggctgggc ccggtcttcc tgcttctggc tggcatcttc ccttttgcac 2341 ctccgggagc tgctgctgag ccccacagtc ttcgttataa cctcacggtg ctgtcctggg 2401 atggatctgt gcagtcaggg tttctcactg aggtacatct ggatggtcag cccttcctgc 2461 gctgtgacag gcagaaatgc agggcaaagc cccagggaca gtgggcagaa gatgtcctgg 2521 gaaataagac atgggacaga gagaccagag acttgacagg gaacggaaag gacctcagga 2581 tgaccctggc tcatatcaag gaccagaaag aaggcttgca ttccctccag gagattaggg 2641 tctgtgagat ccatgaagac aacagcacca ggagctccca gcatttctac tacgatgggg 2701 agctcttcct ctcccaaaac ctggagacta aggaatggac aatgccccag tcctccagag 2761 ctcagacctt ggccatgaac gtcaggaatt tcttgaagga agatgccatg aagaccaaga 2821 cacactatca cgctatgcat gcagactgcc tgcaggaact acggcgatat ctaaaatccg 2881 gcgtagtcct gaggagaaca gtgcccccca tggtgaatgt cacccgcagc gaggcctcag 2941 agggcaacat taccgtgaca tgcagggctt ctggcttcta tccctggaat atcacactga 3001 gctggcgtca ggatggggta tctttgagcc acgacaccca gcagtggggg gatgtcctgc 3061 ctgatgggaa tggaacctac cagacctggg tggccaccag gatttgccaa ggagaggagc 3121 agaggttcac ctgctacatg gaacacagcg ggaatcacag cactcaccct gtgccctctg 3181 ggaaagtgct ggtgcttcag agtcattggc agacattcca tgtttctgct gttgctgctg 3241 ctgctatttt tgttattatt attttctatg tccgttgttg taagaagaaa acatcagctg 3301 cagagggtcc agagctcgtg agcctgcagg tcctggatca acacccagtt gggacgagtg 3361 accacaggga tgccacacag ctcggatttc agcctctgat gtcagatctt gggtccactg 3421 gctccactga gggcgcctag ggatccgcgg ccgcgaagga tctgcgatcg ctccggtgcc 3481 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 3541 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 3601 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 3661 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 3721 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 3781 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 3841 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 3901 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 3961 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagatga ccgagtacaa 4021 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc 4081 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg 4141 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 4201 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 4261 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc 4321 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 4381 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 4441 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc 4501 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 4561 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagtcg acaatcaacc 4621 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 4681 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 4741 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 4801 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 4861 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 4921 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 4981 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt 5041 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 5101 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg 5161 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa 5221 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag 5281 ggctaattca ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt 5341 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 5401 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 5461 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat 5521 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga 5581 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 5641 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 5701 atcatgtctg gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc 5761 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 5821 attccagaag tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa 5881 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 5941 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 6001 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 6061 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 6121 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 6181 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 6241 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 6301 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 6361 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 6421 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 6481 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 6541 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 6601 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 6661 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 6721 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 6781 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 6841 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 6901 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 6961 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 7021 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 7081 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 7141 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 7201 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 7261 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 7321 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 7381 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 7441 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 7501 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 7561 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 7621 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 7681 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 7741 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 7801 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 7861 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 7921 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 7981 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 8041 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 8101 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 8161 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 8221 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 8281 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 8341 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 8401 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 8461 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg //