LOCUS       V040685                10264 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040685
VERSION     V040685
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5118
                     /label="MORC3(NM_015358)"
                     /note="MORC3(NM_015358)"
                     /gene="MORC3"
     CDS             5125..5190
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5234..5445
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5458..5726
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5753..6352
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6359..6947
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7021..7254
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7326..7447
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7466..7601
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7639..7655)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7663..7679
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7687..7717)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7732..7753
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8041..8629)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8800..9660)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9661..9765)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10239..10255
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcggcg cagccacccc gcgggatacg cctcagcgcg
     2341 ctttgcccga agtttttaca tacaaattct actagtcaca cctggccatt cagtgcagtt
     2401 gctgaattaa tagataatgc ttatgatcct gatgtgaacg ctaaacaaat atggattgac
     2461 aaaacagtga taaatgacca tatatgcttg acattcaccg acaatgggaa tggtatgact
     2521 tctgataaat tacataaaat gctaagcttt ggcttcagtg acaaagtcac catgaatggt
     2581 catgtcccag ttggattata tgggaatggc ttcaagtcgg gttctatgcg tctgggtaaa
     2641 gacgcaatcg tttttaccaa aaatggagaa agcatgagcg tgggcctttt gtctcagacc
     2701 tacttggaag tcataaaagc ggagcatgtt gttgttccaa tagtggcatt caacaagcac
     2761 cgacagatga ttaatttagc agaatcaaaa gccagccttg ctgcaattct ggaacattct
     2821 ctgttttcca cggaacagaa gttactggca gaacttgatg ctattatagg caagaagggg
     2881 acgaggatca tcatttggaa tcttagaagc tacaaaaatg caacagagtt cgattttgaa
     2941 aaggataaat atgatatcag aattcccgag gatttagatg agataacagg gaagaagggg
     3001 tacaagaagc aggaaaggat ggaccagatt gcccctgaga gtgactattc cctgagggct
     3061 tattgcagta tattatatct aaagccaaga atgcagatca tcctacgtgg acagaaagtg
     3121 aagacacagc tggtttcgaa gagtcttgcc tacatcgaac gtgatgttta tcgaccaaaa
     3181 tttttatcta aaacagtgag aattaccttt ggattcaact gcagaaataa agatcattat
     3241 gggataatga tgtatcacag aaatagactc atcaaagctt atgaaaaagt tggatgtcag
     3301 ttaagggcaa acaacatggg tgttggagtg gttggaatta tagagtgtaa tttccttaag
     3361 ccaactcata ataaacaaga tttcgactat actaatgagt acagacttac aataacagca
     3421 ctaggagaaa agctgaatga ttactggaat gaaatgaaag tgaagaaaaa tacagaatat
     3481 cctctaaatt tgccagttga agatatacag aagcgtcctg atcagacatg ggttcagtgt
     3541 gatgcctgtc taaagtggcg gaaattacct gatgggatgg atcaacttcc tgaaaaatgg
     3601 tattgctcca ataaccctga cccacagttc agaaattgtg aggttccaga agaacctgaa
     3661 gatgaggatt tggtacatcc cacttatgaa aaaacctaca aaaagaccaa caaggaaaaa
     3721 ttcaggatca gacaaccgga aatgatccct cggattaatg ctgaactgtt gtttcggcca
     3781 actgctcttt caactccaag cttttcttct cctaaggaaa gtgttccaag aagacatctt
     3841 tcagaaggaa caaattctta tgcgacaaga cttctaaata atcatcaagt tccacctcag
     3901 tctgaacctg agagcaacag cttgaaacgg agactttcta ctcgttcctc aattttgaat
     3961 gcaaagaatc ggagattgag tagtcagttt gaaaattcag tttataaagg tgatgatgat
     4021 gatgaagatg tcatcatctt agaagaaaac agtaccccca aacctgcagt agatcatgat
     4081 attgacatga aatcagaaca gagtcacgtt gagcaaggtg gtgttcaggt tgagtttgtg
     4141 ggtgacagtg aaccttgtgg ccagactggt tcaacaagca cctcatcatc ccgatgcgac
     4201 cagggaaata ctgcagctac ccagactgaa gtaccaagtt tagttgttaa aaaagaagaa
     4261 actgttgaag acgagataga cgtaagaaat gatgcagtga ttctgccctc ctgtgtagaa
     4321 gctgaagcaa agatacatga aacccaggaa accaccgata aatctgcaga tgatgcaggc
     4381 tgccaattac aagaactgag aaaccagcta ctccttgtca ctgaggaaaa agagaattat
     4441 aaaagacagt gtcatatgtt tactgatcaa atcaaagtgt tacaacagag gatactagaa
     4501 atgaatgaca agtatgttaa gaaagaaact tgccatcagt ccactgaaac cgatgctgta
     4561 tttttacttg aaagtattaa tggcaaatct gaaagtccag accatatggt atctcagtat
     4621 cagcaagctt tggaagaaat agaaaggctg aaaaaacaat gtagtgcttt gcaacatgta
     4681 aaggctgaat gcagccagtg ttccaataat gagagtaaaa gtgaaatgga tgagatggct
     4741 gtgcagcttg acgatgtgtt tagacaactg gacaaatgca gtattgagag ggaccagtat
     4801 aaaagtgagg ttgaattgct ggaaatggaa aagtcacaaa tccgttcaca gtgtgaagaa
     4861 ctcaaaactg aagtagaaca gttaaaatct acaaatcaac agacggcaac agatgtttca
     4921 acatcaagta acattgagga gtctgtaaat catatggatg gagaaagcct caaactccga
     4981 tctcttcgag ttaacgtagg acaactgctg gctatgattg tgcctgatct tgatcttcag
     5041 caagtgaatt acgatgttga tgtagttgat gagattttag gacaagttgt tgaacaaatg
     5101 agtgaaatca gtagtactgg atccgactac aaagaccatg acggtgatta taaagatcat
     5161 gacatcgact acaaggatga cgatgacaag tagcggccgc gaaggatctg cgatcgctcc
     5221 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     5281 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     5341 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     5401 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     5461 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     5521 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     5581 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     5641 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     5701 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga
     5761 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     5821 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     5881 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5941 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6001 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6061 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6121 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6181 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6241 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6301 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa
     6361 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6421 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6481 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6541 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6601 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6661 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6721 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6781 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6841 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6901 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa
     6961 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7021 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc
     7081 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7141 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7201 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     7261 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7321 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7381 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7441 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc
     7501 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     7561 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg
     7621 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7681 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7741 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7801 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7861 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7921 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7981 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8041 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8101 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8161 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8221 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8281 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8341 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8401 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8461 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     8521 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8581 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8641 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8701 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8761 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8821 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8881 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8941 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9001 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9061 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9121 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9181 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9241 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9301 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9361 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9421 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9481 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9541 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9601 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9661 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9721 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9781 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     9841 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     9901 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     9961 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    10021 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    10081 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    10141 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    10201 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    10261 gctg
//
