LOCUS       V045238                10689 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045238
VERSION     V045238
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2318..5614
                     /label="MRTFB(NM_001308142)"
                     /note="MRTFB(NM_001308142)"
                     /gene="MRTFB"
     promoter        5659..5870
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5883..6151
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6178..6777
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6784..7372
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7446..7679
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7751..7872
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7891..8026
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8064..8080)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8088..8104
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8112..8142)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8157..8178
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8466..9054)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9225..10085)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10086..10190)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10664..10680
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgaatt taaatggatc cgccaccatg gatcacacag gggcgataga
     2341 caccgaggat gaagtgggac ctttagccca tcttgctcca agtcctcaga gtgaagctgt
     2401 ggctcatgaa ttccaggaac tctccttgca gtccagtcaa aacttacccc ctctgaacga
     2461 aaggaaaaat gtgctccagc tgaggctgca acaaaggagg acgagagaac aactagtgga
     2521 ccagggcatc atgccacctt tgaagagccc agcggcattc catgaacaga taaaaagctt
     2581 ggaacgagcc agaactgaaa actttttgaa acacaagatt cggagtcgac cagatcgttc
     2641 tgaacttgtc aggatgcaca ttttagaaga aacatttgca gagccatccc tgcaggctac
     2701 tcagatgaag ttgaaaagag ctcgactagc agatgatctg aatgaaaaga ttgctcaaag
     2761 acctggtcct atggagctgg tagagaaaaa catccttcct gtggactcca gtgttaaaga
     2821 agcaattata ggcgttggga aggaggacta tccccacact cagggcgatt tctcatttga
     2881 tgaagacagc agtgacgctt tgtctccgga ccagcctgcg agtcaggagt cacaggggtc
     2941 agccgcgtcc ccaagtgagc caaaagttag tgaatcgcca tctcctgtga ctacaaacac
     3001 tccagcgcag tttgcttcag tgtccccaac agttcctgaa ttcttgaaaa ctcctccaac
     3061 tgcagatcag cctcccccac ggcctgcagc tcctgtcctc cccacaaaca ctgtgtcctc
     3121 agcaaagcct ggcccagcac tggtgaagca aagccatccc aagaatccaa atgacaaaca
     3181 ccgtagcaaa aagtgcaaag atcccaaacc acgggtaaag aagttaaagt accaccaata
     3241 cattccacca gatcagaagg gtgagaagaa tgagccgcag atggactcta actacgcccg
     3301 cctgctccag cagcagcagc tgttcctgca actgcagatc ctgagtcagc agaagcagca
     3361 ctacaactac cagaccatcc tgcctgcacc attcaagcca ctcaatgaca aaaatagtaa
     3421 cagtgggaat tcagctttga acaatgccac acctaacaca ccaagacaga atacatctac
     3481 tcctgtgaga aagccaggac ctctgccttc tagcctggat gacttaaagg tatcagaact
     3541 gaagacagaa ctgaagttaa ggggtctgcc agtgtcaggc accaaaccgg acctcattga
     3601 gcgcctaaaa ccctaccagg aagtgaacag cagcggcctt gctgctgggg gcatcgtggc
     3661 agtgtcatca tcagccattg tcaccagtaa cccagaagtc actgtggcct tgccggttac
     3721 aacactacac aacactgtga ctagctcagt ctctactctc aaggcagaat tgccacctac
     3781 aggaaccagc aacgcaaccc gtgtggaaaa tgttcattcc cctctgccca tttcaccatc
     3841 tccctccgaa cagtccagtc tcagtactga tgacacaaac atggcagaca ctttcaccga
     3901 gattatgacc atgatgtcgc cttcacagtt cttgagttca tctcctttga gaatgacaaa
     3961 taatgaagac agtctgagtc ccaccagcag cactctgtca aacctggaac tggatgcagc
     4021 cgaaaaggat cgcaagcttc aggagaaaga gaagcaaatc gaagagctga agaggaaact
     4081 ggaacaagag cagaagctcg tggaagtgct gaaaatgcaa cttgaggttg aaaaacgagg
     4141 gcagcagcag cggcccctgg aagcccagcc cagtgcccca ggtcattctg tcaagtcaga
     4201 tcagaagcac ggcagccttg gctcctccat caaagatgag gcctcactcc ctgactgctc
     4261 cagctccagg cagcccatcc cagtagccag ccacgctgta ggccagcccg tctctacagg
     4321 tggccagacc cttgttgcca aaaaggctgt agttatcaag caagaggtcc ctgtgggcca
     4381 ggcagagcag cagagtgtcg tctcgcagtt ttatgtgagt tcccagggac agccaccgcc
     4441 tgctgttgtt gctcagcccc aggctttact gaccacgcag actgctcagc tgctgctccc
     4501 agtgtccatc cagggctcga gtgtcacctc agtgcaactc cctgtaggca gcctcaaact
     4561 ccagacttca ccacaagcag gaatgcagac tcagcctcag atagcaactg ctgcacaaat
     4621 accaactgct gccttggcct caggcttggc cccaactgta cctcagacac aagacacgtt
     4681 cccgcagcat gtgctcagtc agcctcaaca agtcagaaag gttttcacaa actcagcatc
     4741 atcaaataca gttcttccat atcagagaca tcctgcccca gctgtccagc agccctttat
     4801 caataaggcc tccaacagtg ttcttcaatc cagaaatgct ccgcttccat ccctgcaaaa
     4861 tggacctaac acacccaaca agcctagttc acccccgcca ccccagcaat ttgtcgtcca
     4921 gcactctcta tttgggagtc cagtcgccaa gacaaaagat cccccccgct atgaggaggc
     4981 catcaagcag acacgcagca cacaggcccc tctgccagag atttccaacg ctcacagtca
     5041 gcagatggat gacctctttg atatcctcat taagagtgga gagatctccc tccccataaa
     5101 agaagaacct tctcctattt ccaaaatgag accagtgaca gccagcatca ccacaatgcc
     5161 agtgaataca gtggtgtccc ggccaccacc ccaagtccaa atggcaccac ctgtatcttt
     5221 agaacctatg ggcagtttat ctgccagctt agagaaccaa ctagaagctt tcttggatgg
     5281 aactttaccc tcagccaatg aaattcctcc actacaaagc agcagtgaag acagagagcc
     5341 cttctctctg atcgaggacc tccagaatga tctgctgagt cactcaggta tgctggacca
     5401 ttcacactca cccatggaga cttccgagac ccagtttgct gcaggtactc cctgtctgtc
     5461 tctcgacctg tcagactcaa acttggacaa catggagtgg ttggacatta ccatgcccaa
     5521 ctcctcttca ggactcactc ctctcagcac caccgcgccg agcatgttct ctgctgactt
     5581 tctagaccca caggacctac cgctgccatg ggactaagcg gccgcgaagg atctgcgatc
     5641 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     5701 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     5761 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     5821 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc
     5881 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc
     5941 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct
     6001 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac
     6061 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg
     6121 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagatg
     6181 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc
     6241 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc
     6301 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6361 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6421 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6481 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     6541 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     6601 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     6661 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     6721 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtc
     6781 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6841 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6901 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6961 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7021 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7081 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7141 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7201 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7261 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7321 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggtacc
     7381 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7441 gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg
     7501 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7561 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7621 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7681 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     7741 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7801 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7861 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc
     7921 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     7981 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga
     8041 gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8101 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8161 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8221 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8281 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8341 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8401 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8461 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8521 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8581 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8641 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8701 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8761 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8821 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8881 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     8941 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9001 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9061 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9121 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9181 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9241 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9301 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9361 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9421 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9481 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9541 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9601 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9661 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9721 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9781 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9841 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9901 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9961 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10021 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10081 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10141 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10201 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
    10261 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
    10321 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    10381 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
    10441 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
    10501 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
    10561 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    10621 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    10681 gccaagctg
//
