LOCUS       V037896                15819 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037896
VERSION     V037896
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2303..9949
                     /label="MTOR(NM_004958)"
                     /note="MTOR(NM_004958)"
                     /gene="MTOR"
     CDS             9956..9973
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     promoter        10017..10228
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             10241..10509
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             10544..11251
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             11261..11314
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             11315..11914
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    11915..12502
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             12576..12809
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    12881..13002
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      13021..13156
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(13194..13210)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    13218..13234
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(13242..13272)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    13287..13308
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(13596..14184)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(14355..15215)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(15216..15320)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     15794..15810
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc gaattcgcca ccatgcttgg aaccggacct gccgccgcca ccaccgctgc
     2341 caccacatct agcaatgtga gcgtcctgca gcagtttgcc agtggcctaa agagccggaa
     2401 tgaggaaacc agggccaaag ccgccaagga gctccagcac tatgtcacca tggaactccg
     2461 agagatgagt caagaggagt ctactcgctt ctatgaccaa ctgaaccatc acatttttga
     2521 attggtttcc agctcagatg ccaatgagag gaaaggtggc atcttggcca tagctagcct
     2581 cataggagtg gaaggtggga atgccacccg aattggcaga tttgccaact atcttcggaa
     2641 cctcctcccc tccaatgacc cagttgtcat ggaaatggca tccaaggcca ttggccgtct
     2701 tgccatggca ggggacactt ttaccgctga gtacgtggaa tttgaggtga agcgagccct
     2761 ggaatggctg ggtgctgacc gcaatgaggg ccggagacat gcagctgtcc tggttctccg
     2821 tgagctggcc atcagcgtcc ctaccttctt cttccagcaa gtgcaaccct tctttgacaa
     2881 catttttgtg gccgtgtggg accccaaaca ggccatccgt gagggagctg tagccgccct
     2941 tcgtgcctgt ctgattctca caacccagcg tgagccgaag gagatgcaga agcctcagtg
     3001 gtacaggcac acatttgaag aagcagagaa gggatttgat gagaccttgg ccaaagagaa
     3061 gggcatgaat cgggatgatc ggatccatgg agccttgttg atccttaacg agctggtccg
     3121 aatcagcagc atggagggag agcgtctgag agaagaaatg gaagaaatca cacagcagca
     3181 gctggtacac gacaagtact gcaaagatct catgggcttc ggaacaaaac ctcgtcacat
     3241 tacccccttc accagtttcc aggctgtaca gccccagcag tcaaatgcct tggtggggct
     3301 gctggggtac agctctcacc aaggcctcat gggatttggg acctccccca gtccagctaa
     3361 gtccaccctg gtggagagcc ggtgttgcag agacttgatg gaggagaaat ttgatcaggt
     3421 gtgccagtgg gtgctgaaat gcaggaatag caagaactcg ctgatccaaa tgacaatcct
     3481 taatttgttg ccccgcttgg ctgcattccg accttctgcc ttcacagata cccagtatct
     3541 ccaagatacc atgaaccatg tcctaagctg tgtcaagaag gagaaggaac gtacagcggc
     3601 cttccaagcc ctggggctac tttctgtggc tgtgaggtct gagtttaagg tctatttgcc
     3661 tcgcgtgctg gacatcatcc gagcggccct gcccccaaag gacttcgccc ataagaggca
     3721 gaaggcaatg caggtggatg ccacagtctt cacttgcatc agcatgctgg ctcgagcaat
     3781 ggggccaggc atccagcagg atatcaagga gctgctggag cccatgctgg cagtgggact
     3841 aagccctgcc ctcactgcag tgctctacga cctgagccgt cagattccac agctaaagaa
     3901 ggacattcaa gatgggctac tgaaaatgct gtccctggtc cttatgcaca aaccccttcg
     3961 ccacccaggc atgcccaagg gcctggccca tcagctggcc tctcctggcc tcacgaccct
     4021 ccctgaggcc agcgatgtgg gcagcatcac tcttgccctc cgaacgcttg gcagctttga
     4081 atttgaaggc cactctctga cccaatttgt tcgccactgt gcggatcatt tcctgaacag
     4141 tgagcacaag gagatccgca tggaggctgc ccgcacctgc tcccgcctgc tcacaccctc
     4201 catccacctc atcagtggcc atgctcatgt ggttagccag accgcagtgc aagtggtggc
     4261 agatgtgctt agcaaactgc tcgtagttgg gataacagat cctgaccctg acattcgcta
     4321 ctgtgtcttg gcgtccctgg acgagcgctt tgatgcacac ctggcccagg cggagaactt
     4381 gcaggccttg tttgtggctc tgaatgacca ggtgtttgag atccgggagc tggccatctg
     4441 cactgtgggc cgactcagta gcatgaaccc tgcctttgtc atgcctttcc tgcgcaagat
     4501 gctcatccag attttgacag agttggagca cagtgggatt ggaagaatca aagagcagag
     4561 tgcccgcatg ctggggcacc tggtctccaa tgccccccga ctcatccgcc cctacatgga
     4621 gcctattctg aaggcattaa ttttgaaact gaaagatcca gaccctgatc caaacccagg
     4681 tgtgatcaat aatgtcctgg caacaatagg agaattggca caggttagtg gcctggaaat
     4741 gaggaaatgg gttgatgaac tttttattat catcatggac atgctccagg attcctcttt
     4801 gttggccaaa aggcaggtgg ctctgtggac cctgggacag ttggtggcca gcactggcta
     4861 tgtagtagag ccctacagga agtaccctac tttgcttgag gtgctactga attttctgaa
     4921 gactgagcag aaccagggta cacgcagaga ggccatccgt gtgttagggc ttttaggggc
     4981 tttggatcct tacaagcaca aagtgaacat tggcatgata gaccagtccc gggatgcctc
     5041 tgctgtcagc ctgtcagaat ccaagtcaag tcaggattcc tctgactata gcactagtga
     5101 aatgctggtc aacatgggaa acttgcctct ggatgagttc tacccagctg tgtccatggt
     5161 ggccctgatg cggatcttcc gagaccagtc actctctcat catcacacca tggttgtcca
     5221 ggccatcacc ttcatcttca agtccctggg actcaaatgt gtgcagttcc tgccccaggt
     5281 catgcccacg ttccttaacg tcattcgagt ctgtgatggg gccatccggg aatttttgtt
     5341 ccagcagctg ggaatgttgg tgtcctttgt gaagagccac atcagacctt atatggatga
     5401 aatagtcacc ctcatgagag aattctgggt catgaacacc tcaattcaga gcacgatcat
     5461 tcttctcatt gagcaaattg tggtagctct tgggggtgaa tttaagctct acctgcccca
     5521 gctgatccca cacatgctgc gtgtcttcat gcatgacaac agcccaggcc gcattgtctc
     5581 tatcaagtta ctggctgcaa tccagctgtt tggcgccaac ctggatgact acctgcattt
     5641 actgctgcct cctattgtta agttgtttga tgcccctgaa gctccactgc catctcgaaa
     5701 ggcagcgcta gagactgtgg accgcctgac ggagtccctg gatttcactg actatgcctc
     5761 ccggatcatt caccctattg ttcgaacact ggaccagagc ccagaactgc gctccacagc
     5821 catggacacg ctgtcttcac ttgtttttca gctggggaag aagtaccaaa ttttcattcc
     5881 aatggtgaat aaagttctgg tgcgacaccg aatcaatcat cagcgctatg atgtgctcat
     5941 ctgcagaatt gtcaagggat acacacttgc tgatgaagag gaggatcctt tgatttacca
     6001 gcatcggatg cttaggagtg gccaagggga tgcattggct agtggaccag tggaaacagg
     6061 acccatgaag aaactgcacg tcagcaccat caacctccaa aaggcctggg gcgctgccag
     6121 gagggtctcc aaagatgact ggctggaatg gctgagacgg ctgagcctgg agctgctgaa
     6181 ggactcatca tcgccctccc tgcgctcctg ctgggccctg gcacaggcct acaacccgat
     6241 ggccagggat ctcttcaatg ctgcatttgt gtcctgctgg tctgaactga atgaagatca
     6301 acaggatgag ctcatcagaa gcatcgagtt ggccctcacc tcacaagaca tcgctgaagt
     6361 cacacagacc ctcttaaact tggctgaatt catggaacac agtgacaagg gccccctgcc
     6421 actgagagat gacaatggca ttgttctgct gggtgagaga gctgccaagt gccgagcata
     6481 tgccaaagca ctacactaca aagaactgga gttccagaaa ggccccaccc ctgccattct
     6541 agaatctctc atcagcatta ataataagct acagcagccg gaggcagcgg ccggagtgtt
     6601 agaatatgcc atgaaacact ttggagagct ggagatccag gctacctggt atgagaaact
     6661 gcacgagtgg gaggatgccc ttgtggccta tgacaagaaa atggacacca acaaggacga
     6721 cccagagctg atgctgggcc gcatgcgctg cctcgaggcc ttgggggaat ggggtcaact
     6781 ccaccagcag tgctgtgaaa agtggaccct ggttaatgat gagacccaag ccaagatggc
     6841 ccggatggct gctgcagctg catggggttt aggtcagtgg gacagcatgg aagaatacac
     6901 ctgtatgatc cctcgggaca cccatgatgg ggcattttat agagctgtgc tggcactgca
     6961 tcaggacctc ttctccttgg cacaacagtg cattgacaag gccagggacc tgctggatgc
     7021 tgaattaact gcgatggcag gagagagtta cagtcgggca tatggggcca tggtttcttg
     7081 ccacatgctg tccgagctgg aggaggttat ccagtacaaa cttgtccccg agcgacgaga
     7141 gatcatccgc cagatctggt gggagagact gcagggctgc cagcgtatcg tagaggactg
     7201 gcagaaaatc cttatggtgc ggtcccttgt ggtcagccct catgaagaca tgagaacctg
     7261 gctcaagtat gcaagcctgt gcggcaagag tggcaggctg gctcttgctc ataaaacttt
     7321 agtgttgctc ctgggagttg atccgtctcg gcaacttgac catcctctgc caacagttca
     7381 ccctcaggtg acctatgcct acatgaaaaa catgtggaag agtgcccgca agatcgatgc
     7441 cttccagcac atgcagcatt ttgtccagac catgcagcaa caggcccagc atgccatcgc
     7501 tactgaggac cagcagcata agcaggaact gcacaagctc atggcccgat gcttcctgaa
     7561 acttggagag tggcagctga atctacaggg catcaatgag agcacaatcc ccaaagtgct
     7621 gcagtactac agcgccgcca cagagcacga ccgcagctgg tacaaggcct ggcatgcgtg
     7681 ggcagtgatg aacttcgaag ctgtgctaca ctacaaacat cagaaccaag cccgcgatga
     7741 gaagaagaaa ctgcgtcatg ccagcggggc caacatcacc aacgccacca ctgccgccac
     7801 cacggccgcc actgccacca ccactgccag caccgagggc agcaacagtg agagcgaggc
     7861 cgagagcacc gagaacagcc ccaccccatc gccgctgcag aagaaggtca ctgaggatct
     7921 gtccaaaacc ctcctgatgt acacggtgcc tgccgtccag ggcttcttcc gttccatctc
     7981 cttgtcacga ggcaacaacc tccaggatac actcagagtt ctcaccttat ggtttgatta
     8041 tggtcactgg ccagatgtca atgaggcctt agtggagggg gtgaaagcca tccagattga
     8101 tacctggcta caggttatac ctcagctcat tgcaagaatt gatacgccca gacccttggt
     8161 gggacgtctc attcaccagc ttctcacaga cattggtcgg taccaccccc aggccctcat
     8221 ctacccactg acagtggctt ctaagtctac cacgacagcc cggcacaatg cagccaacaa
     8281 gattctgaag aacatgtgtg agcacagcaa caccctggtc cagcaggcca tgatggtgag
     8341 cgaggagctg atccgagtgg ccatcctctg gcatgagatg tggcatgaag gcctggaaga
     8401 ggcatctcgt ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc
     8461 cttgcatgct atgatggaac ggggccccca gactctgaag gaaacatcct ttaatcaggc
     8521 ctatggtcga gatttaatgg aggcccaaga gtggtgcagg aagtacatga aatcagggaa
     8581 tgtcaaggac ctcacccaag cctgggacct ctattatcat gtgttccgac gaatctcaaa
     8641 gcagctgcct cagctcacat ccttagagct gcaatatgtt tccccaaaac ttctgatgtg
     8701 ccgggacctt gaattggctg tgccaggaac atatgacccc aaccagccaa tcattcgcat
     8761 tcagtccata gcaccgtctt tgcaagtcat cacatccaag cagaggcccc ggaaattgac
     8821 acttatgggc agcaacggac atgagtttgt tttccttcta aaaggccatg aagatctgcg
     8881 ccaggatgag cgtgtgatgc agctcttcgg cctggttaac acccttctgg ccaatgaccc
     8941 aacatctctt cggaaaaacc tcagcatcca gagatacgct gtcatccctt tatcgaccaa
     9001 ctcgggcctc attggctggg ttccccactg tgacacactg cacgccctca tccgggacta
     9061 cagggagaag aagaagatcc ttctcaacat cgagcatcgc atcatgttgc ggatggctcc
     9121 ggactatgac cacttgactc tgatgcagaa ggtggaggtg tttgagcatg ccgtcaataa
     9181 tacagctggg gacgacctgg ccaagctgct gtggctgaaa agccccagct ccgaggtgtg
     9241 gtttgaccga agaaccaatt atacccgttc tttagcggtc atgtcaatgg ttgggtatat
     9301 tttaggcctg ggagatagac acccatccaa cctgatgctg gaccgtctga gtgggaagat
     9361 cctgcacatt gactttgggg actgctttga ggttgctatg acccgagaga agtttccaga
     9421 gaagattcca tttagactaa caagaatgtt gaccaatgct atggaggtta caggcctgga
     9481 tggcaactac agaatcacat gccacacagt gatggaggtg ctgcgagagc acaaggacag
     9541 tgtcatggcc gtgctggaag cctttgtcta tgaccccttg ctgaactgga ggctgatgga
     9601 cacaaatacc aaaggcaaca agcgatcccg aacgaggacg gattcctact ctgctggcca
     9661 gtcagtcgaa attttggacg gtgtggaact tggagagcca gcccataaga aaacggggac
     9721 cacagtgcca gaatctattc attctttcat tggagacggt ttggtgaaac cagaggccct
     9781 aaataagaaa gctatccaga ttattaacag ggttcgagat aagctcactg gtcgggactt
     9841 ctctcatgat gacactttgg atgttccaac gcaagttgag ctgctcatca aacaagcgac
     9901 atcccatgaa aacctctgcc agtgctatat tggctggtgc cctttctggg gatcccatca
     9961 tcatcatcat cactagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
    10021 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
    10081 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
    10141 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
    10201 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
    10261 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
    10321 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
    10381 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
    10441 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
    10501 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggtga gcaagggcga
    10561 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
    10621 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
    10681 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
    10741 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
    10801 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
    10861 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
    10921 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
    10981 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
    11041 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
    11101 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
    11161 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
    11221 ctccaccggc ggcatggacg agctgtacaa gggatctcgc gagggcagag gaagtcttct
    11281 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg
    11341 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
    11401 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
    11461 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
    11521 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
    11581 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
    11641 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
    11701 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
    11761 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
    11821 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
    11881 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg
    11941 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
    12001 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
    12061 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
    12121 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
    12181 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
    12241 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
    12301 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
    12361 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
    12421 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat
    12481 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
    12541 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
    12601 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
    12661 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    12721 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    12781 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
    12841 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
    12901 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    12961 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
    13021 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    13081 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    13141 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag
    13201 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    13261 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    13321 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    13381 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    13441 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    13501 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    13561 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    13621 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    13681 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    13741 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    13801 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    13861 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    13921 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    13981 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    14041 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    14101 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    14161 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    14221 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    14281 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    14341 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    14401 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    14461 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    14521 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    14581 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    14641 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    14701 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    14761 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    14821 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    14881 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    14941 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    15001 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    15061 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    15121 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    15181 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    15241 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    15301 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    15361 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    15421 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    15481 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    15541 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    15601 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    15661 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    15721 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    15781 cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
