LOCUS       V021230                10770 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021230
VERSION     V021230
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2301..5624
                     /label="MYO1E(NM_004998)"
                     /note="MYO1E(NM_004998)"
                     /gene="MYO1E"
     CDS             5631..5696
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5740..5951
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5964..6232
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6259..6858
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6865..7453
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7527..7760
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7832..7953
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7972..8107
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8145..8161)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8169..8185
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8193..8223)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8238..8259
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8547..9135)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9306..10166)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10167..10271)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10745..10761
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagattcta
     2281 gagctagcga attcgccacc atgggaagca aaggtgtcta ccagtaccac tggcaaagcc
     2341 acaatgtcaa gcacagtggt gtggacgaca tggtgctact gtccaagatc acagagaact
     2401 ccatcgtgga gaatctgaag aagagataca tggatgacta catttttaca tatataggat
     2461 ctgtattaat ctcagtcaac cctttcaagc agatgccata ttttggggaa aaggaaattg
     2521 aaatgtacca aggagcggca cagtatgaaa acccaccaca tatctatgcc cttgcagata
     2581 atatgtacag aaacatgatc attgacagag agaaccagtg cgtcattatc agtggtgaaa
     2641 gtggtgctgg aaaaacagtg gctgccaaat atatcatgag ctacatctcc agagtgtctg
     2701 gaggagggac caaagtccag cacgtgaagg acattatcct gcagtccaac ccgctgctgg
     2761 aggccttcgg gaacgccaag accgtccgga acaacaactc cagccgattt ggaaaatact
     2821 ttgaaatcca gttcagtcca ggtggggaac cagatggtgg aaagatctcc aacttccttc
     2881 tggaaaaatc tagggtggtg atgaggaacc caggagagcg gagttttcac atattttacc
     2941 agctcatcga gggcgcctct gcagagcaga aacacagcct tggcatcacc agcatggact
     3001 attattacta cctgagcctc tcgggctcat acaaggttga tgacattgac gacaggcggg
     3061 agtttcagga aactctgcac gccatgaatg tgattgggat ctttgcagaa gagcaaacgc
     3121 tggtgttgca gatagtggcg ggtattctcc acctgggaaa catcagcttc aaagaagttg
     3181 gcaactacgc ggctgtggag agtgaagagt ttttagcttt tcctgcatat ctgctaggga
     3241 taaaccagga ccggttgaaa gaaaagctaa caagccggca gatggatagc aagtggggag
     3301 gcaaatccga atccatccac gtgaccctca acgtagagca ggcctgttac acccgggatg
     3361 cgctcgccaa ggccctgcac gcccgggtct ttgatttctt ggtagattcc atcaataaag
     3421 ccatggagaa agaccatgaa gaatacaaca ttggcgtcct agacatctat ggctttgaaa
     3481 tattccagaa aaatggcttt gaacagtttt gtatcaattt tgttaatgaa aaactgcagc
     3541 agatttttat tgaactgaca ttaaaggcag aacaggaaga atatgttcaa gagggaataa
     3601 gatggacacc cattgagtac tttaataata aaatcgtatg tgacctcata gagaacaaag
     3661 tgaaccctcc tggcatcatg agcatcctgg atgacgtgtg cgccacgatg catgcggtgg
     3721 gtgagggggc agatcagacg ctgctccaga aacttcagat gcagattggg agtcatgagc
     3781 acttcaacag ttggaaccaa ggcttcatca ttcatcatta tgctgggaag gtatcctatg
     3841 acatggatgg cttttgtgaa aggaaccggg atgtgctttt tatggatctc atcgagctta
     3901 tgcagagcag cgagctgcct ttcataaagt ctttatttcc ggaaaatctg caggctgaca
     3961 agaaagggcg cccaactact gccggaagca aaataaagaa acaagccaat gaccttgtga
     4021 gcaccctgat gaaatgtacg ccccactaca ttcgctgcat caagccaaac gaaaccaaga
     4081 agcccagaga ctgggaggaa agcagggtaa agcatcaagt cgaatatttg ggtctgaaag
     4141 agaacattcg agtgagaaga gctggctatg cctatcggcg catcttccaa aaattcctac
     4201 agaggtatgc cattctgacc aaagccacct ggccttcttg gcagggagag gagaagcaag
     4261 gcgtcctgca cctgctgcag tcggtcaaca tggacagcga ccagttccag ctggggagga
     4321 gtaaagtgtt catcaaagcc cccgagtctc tatttctttt agaagagatg agagagagaa
     4381 agtatgatgg gtatgctcga gtgatacaga aatcatggag gaaattcgtg gcccggaaga
     4441 aatacgttca aatgagagaa gaagcctcag acctcttatt gaacaagaag gagagaagga
     4501 gaaacagtat taacaggaac tttatagggg attatattgg gatggaagag cacccagaac
     4561 tccagcagtt cgtgggcaag agggagaaga ttgatttcgc agacacagtc accaagtatg
     4621 acaggaggtt caagggtgta aagcgagacc tgctccttac cccaaagtgc ttgtacttaa
     4681 tcggacgaga aaaagtcaaa cagggcccag acaagggcct ggtgaaagaa gtcctgaagc
     4741 ggaaaatcga gatagaacgg atcttgtctg tgtccctcag tactatgcag gatgacattt
     4801 ttattctcca tgagcaagag tatgacagtt tgcttgaatc tgtcttcaaa actgaattcc
     4861 taagcctctt agcaaagcgt tacgaggaga agacccagaa gcaactacct ctgaaattca
     4921 gcaatacgct tgaactgaag ttgaaaaagg aaaactgggg cccctggagt gcagggggct
     4981 cccggcaagt gcagttccac caagggtttg gggacctggc tgtcctcaag cccagtaaca
     5041 aagtgctgca ggtcagcatc ggacctggac tgcccaagaa ctcccgtcct accagaagga
     5101 acactaccca aaatacaggt tattccagtg ggactcaaaa tgccaactac ccagtgagag
     5161 ctgcccctcc tcccccagga taccatcaga acggagtcat cagaaaccag tatgtgccat
     5221 atccccatgc tcctggaagc cagaggtcca atcagaaaag cctgtacacc tccatggccc
     5281 gcccgccctt gcctcggcag cagtctacca gttcagaccg agtgtcacag acgccagaga
     5341 gcctggattt cctcaaggtc ccggaccagg gagctgcagg ggtcaggaga caaacaacca
     5401 gtcggcctcc cccagcaggg ggcagaccca agccccagcc caagcccaag cctcaggtgc
     5461 cacagtgcaa ggctttgtat gcctatgacg ctcaggacac agacgaactc agctttaatg
     5521 ccaatgacat tattgatatt atcaaagaag atccttctgg ctggtggacg ggtcgactac
     5581 gaggcaagca gggcctgttc cccaacaact atgtgaccaa gatcggatcc gactacaaag
     5641 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagc
     5701 ggccgcgaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     5761 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     5821 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     5881 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     5941 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     6001 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     6061 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     6121 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     6181 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     6241 gaccggcgcc tacgctagat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6301 gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6361 cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6421 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6481 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6541 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6601 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6661 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6721 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6781 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6841 cgcaagcccg gtgcctgagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     6901 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6961 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7021 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7081 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7141 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7201 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7261 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7321 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7381 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7441 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7501 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga
     7561 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7621 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7681 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7741 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7801 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7861 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7921 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7981 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8041 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8101 ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct
     8161 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8221 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8281 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8341 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8401 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8461 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8521 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8581 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8641 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8701 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8761 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8821 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8881 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8941 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     9001 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9061 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9121 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9181 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9241 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9301 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9361 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9421 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9481 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9541 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9601 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9661 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9721 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9781 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9841 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9901 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9961 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10021 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10081 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10141 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10201 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10261 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    10321 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    10381 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    10441 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    10501 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    10561 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
    10621 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    10681 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    10741 cgttgtaaaa cgacggccag tgccaagctg
//
