LOCUS       V024853                11729 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V024853
VERSION     V024853
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5772
                     /label="Man2a2(NM_172903)"
                     /note="Man2a2(NM_172903)"
                     /gene="Man2a2"
     CDS             5779..5844
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5888..6099
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6112..6380
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6409..6418
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6439..7101
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7171..7224
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7225..7824
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7825..8412
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8486..8719
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8791..8912
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8931..9066
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9104..9120)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9128..9144
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9152..9182)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9197..9218
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9506..10094)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10265..11125)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11126..11230)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11704..11720
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatga agctgaaaaa gcaggtgacg
     2341 gtgtgtgggg ctgctatctt ctgtgtggcc gtcttttctc tgtaccttat gctggaccga
     2401 gtgcagcatg atcctgccag acaccagaat ggtgggaact tccccaggag ccaaatctct
     2461 gtgctacaga accggataga acagctggaa caactgctgg aggaaaacca tgacatcata
     2521 agccgtatca aggactctgt actggaactg acagccaatg cggagggccc gccagccctg
     2581 ctgccctacc ataccgccaa cggttcctgg gctgtgctcc cggagccccg gcccagcttc
     2641 ttctccgtct ccccccagga ctgccagttt gctttggggg gacggggtca gaagccagaa
     2701 ctacagatgt taaccgtgtc tgaggatttg ccgtttgaca atgtggaggg tggcgtgtgg
     2761 aggcaaggct ttgacatctc ctacagccca aatgactggg acactgaaga cctgcaagtg
     2821 tttgtggtgc ctcactccca caacgatcca ggctggatca agacttttga caagtactac
     2881 acggaacaga cccagcacat cctcaacagc atggtgtcca agctgcagga agacccccgg
     2941 cggcgctttc tctgggcaga agtctccttc ttcgccaagt ggtgggacaa catcagtgcc
     3001 caaaagaggg cagcagttcg aaggctggtg ggaaatgggc agctggagat tgcgacaggc
     3061 ggatgggtga tgcccgacga ggccaactcc cattactttg ccttggttga ccagctcatc
     3121 gaggggcacc agtggctgga gagaaacctg ggtgcaaccc cgcgctcggg ctgggcagtg
     3181 gacccctttg gacacagctc caccatgcct tacctgctgc gccgtgccaa cctgaccagc
     3241 atgctgattc agagggtgca ttatgccatc aagaagcact ttgctgccac tcatagcctg
     3301 gagttcatgt ggaggcagat gtgggattca gactccagca cagacatctt ttgccacatg
     3361 atgcccttct acagctacga cgttccacac acctgtggcc ccgatcccaa gatctgctgc
     3421 cagtttgatt tcaaacgtct gccgggtggg agaatcaatt gcccttggaa ggtgccaccc
     3481 cgggctatta cagaagccaa tgtggcagac agggcagccc tgctcctgga ccagtaccgg
     3541 aagaagtccc ggctgttccg aagcaacgtc ctcctcgtgc cacttggtga tgacttccgg
     3601 tatgacaagc cccaggaatg ggatgcccag ttcttcaact accagcggct ctttgacttc
     3661 ctcaacagca agcctgagtt ccatgtgcag gcccagtttg ggaccctctc tgagtatttc
     3721 gatgccctgt ataagaggac aggagtagag cctggtgccc ggcctccagg gtttcctgtg
     3781 ctgagtgggg acttcttctc ctacgctgac cgggaggacc actactggac aggctattac
     3841 acctcccggc ctttctacaa gagcttggac cgcgtcctag aagcccacct gcgtggggca
     3901 gagattctat acagcctggc tttggcccat gcccgccgtt ctggactggc cggccagtac
     3961 ccgctgtctg attttgctct tctgacggaa gctcggcgta cactgggcct cttccagcac
     4021 cacgatgcca tcacgggaac cgccaaggag gcagtagtgg tagactacgg ggtcaggttg
     4081 ctgcgttccc tggtcagcct caagcaggtt atcatcaatg ctgcccacta cctggtgctg
     4141 ggggaccagg agacctacag ctttgaccct gggacaccct tcctccaaat ggatgacagc
     4201 cgtgtaagcc atgatgccct gcccgagcgc acggtgatcc ggctggactc ctcacccagg
     4261 tttgtggtgg tatttaaccc gctggaacag gagcggctca gtgtggtgtc cctgctggtc
     4321 aactcgcccc gggtgcgcgt cctttcagag gagggccagc ccctgtctgt gcagatcagt
     4381 gtgcactgga gctcggctac ggacatggtc cctgatgtct accaggtgtc agtacccgtc
     4441 cgcctgccag gcctgggcct gggtgtgctg cagctgcagc cagatctcga tgggccctac
     4501 acgctgcagt cttccgtgcg ggtctacctg aacggcgtga aactgtccgt cagcaggcag
     4561 tcagcattcc ctgtccgtgt tgtggactcc ggcgccagcg acttcgccat cagcaatcgc
     4621 tacatgcagg tctggttctc cggccttact gggcttctca agagcatccg acgggtggat
     4681 gaggagcagg agcagcagat ggaactggag ttcctcgtct acggcacccg tacctccaag
     4741 gacaagagtg gcgcctacct cttcctgcct gatagcgagg ctaagcccta cgtccctaag
     4801 aaacctcctg tgctgcgtgt caccgaaggc cctttcttct cagaggtggc tgtgtactat
     4861 gagcactttc accaagtgat tcgactttat aacctgccag gggtagaggg gctgtctctg
     4921 gacatgtcat tccaggtaga tatcagggac tatgtgaaca aggaactggc cctgcgcatc
     4981 cacacagaca ttgacagcca gggcactttc ttcacagacc tcaatggctt tcagatacag
     5041 ccccggcagt atctgaagaa gctgcccctg caggctaatt tctaccctat gccagtcatg
     5101 gcctatatcc aagattccca gaggcgcctc acgctgcaca ctgcccaggc tctgggtgtc
     5161 tccagcctcg gtaatggcca gctggaggtg atcttggacc gaaggctaat gcaggatgac
     5221 aaccggggac taggccaagg gctcaaagac aacaagatca cctgcaaccg tttccgcctc
     5281 ctgttagaac gtcggaccac aatgagccca gaggtgcatc aggagcagga gcgttctaca
     5341 agctacccgt ccctcctcag ccacctgaca tccatgtacc tcagcacacc tcctcttgtc
     5401 ttacccgtgg ccaagaggca gggcactagc cctgctctgc gctctttcca ccctctggct
     5461 tctccattgc cctgtgactt ccatttgctc aatctgcgca tgctcccagc cgaggacacc
     5521 ttgcccgcaa ctgactctgc gctcatctta caccgcaagg ggtttgactg tggccttgaa
     5581 gccaagaacc tgggcttcaa ctgtaccaca agccaaggca agctagccct ggggagcctc
     5641 ttccatggcc tggatgtgac cttcctgcag ccaacctcct tgactttgct ataccctctg
     5701 gcctcacctt ccaacagcac tgacatctct ctggagccca tggagatcag caccttccgc
     5761 ctgcgcttgg gcggatccga ctacaaagac catgacggtg attataaaga tcatgacatc
     5821 gactacaagg atgacgatga caagtagcgg ccgcgaagga tctgcgatcg ctccggtgcc
     5881 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     5941 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     6001 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     6061 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     6121 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     6181 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     6241 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     6301 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     6361 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag
     6421 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac
     6481 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc
     6541 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg
     6601 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg
     6661 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc
     6721 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag
     6781 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc
     6841 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc
     6901 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac
     6961 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc
     7021 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc
     7081 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc
     7141 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt
     7201 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc
     7261 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7321 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7381 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7441 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7501 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7561 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     7621 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     7681 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     7741 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     7801 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg
     7861 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7921 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7981 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     8041 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     8101 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     8161 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     8221 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8281 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8341 gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg
     8401 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8461 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat
     8521 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8581 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8641 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8701 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     8761 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     8821 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8881 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     8941 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9001 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9061 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg
     9121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9181 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9241 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9301 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9421 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9481 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     9661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     9721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     9781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     9841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     9961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10021 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10081 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10141 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10201 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10261 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10321 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10381 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10441 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10501 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10561 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10621 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    10681 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    10741 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    10801 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    10861 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    10921 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    10981 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11041 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11101 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11161 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11221 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
    11281 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
    11341 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
    11401 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
    11461 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
    11521 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
    11581 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
    11641 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
    11701 gttgtaaaac gacggccagt gccaagctg
//
