LOCUS       V029186                10369 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029186
VERSION     V029186
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4395
                     /label="Marchf7(NM_020575)"
                     /note="Marchf7(NM_020575)"
                     /gene="Marchf7"
     CDS             4402..4467
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4511..4722
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4735..5003
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5032..5041
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5062..5724
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5794..5847
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5848..6447
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6448..7036
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7110..7343
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7415..7536
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7576..7711
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7744..7760)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7768..7784
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7792..7822)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7837..7858
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8146..8734)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8905..9765)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9766..9870)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10344..10360
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agtctaaacc ttccaggatt
     2341 ccaagaagaa tttctgttca accctctggc tctttaagca ctaggatggt gtctggaaac
     2401 agaggaacca gtttaaatga ttcatatcat tctagagact cctcctttag actggattct
     2461 gaatatcagt ctgcatcagc atcagcgtgt gcatcaccat gtcagcctgc ctggtacagt
     2521 gagtctgaga tacctcaggg agcgcgggca cgagcacaga cccagcagcg ggatcatgac
     2581 tcaaagagac ccaagctttc ctgtacaaac tgtgcatcta cctcagctgg gaggaacggt
     2641 gggagtgggt taaatacagt gtcagattct tcttggaggc atagtcaagt tcccagatct
     2701 tcatcaatgg tacttggttc atttggaaca gacttgatga gagaaaggag agatttggac
     2761 aggagaagag agtcctccat cagcaatctt atggattata atcaccgaag tggtgatttc
     2821 acaacttcat catatgttca agaaagagtt ccttcttcat attcacaggg agcaagacca
     2881 aaagagaatg cagtgagcac tttacagttg aattcatcat ccaccaatca ccaattgcct
     2941 tctgaccatc agacagtacc aagttctagg gactccagta gaagttcttt cagatcacat
     3001 ttttctccaa gacaatcaga atcttttcgc aacagttcac atcctgcatt ttcatatttt
     3061 tcaagtagaa atgaaactcc aactataagc aattcagaaa ggggttcatc tcagagacca
     3121 tatcgagaat cttctgacaa tgaaggtagg cgtacaacta ggagattgct gtcacggata
     3181 gcttctagca tgtcatctac ttttttctca cgaagatcta gtcaagattc cttgaataca
     3241 agatctttga gttctgaaaa ttatatttct ccgagaaccc tgacttcaca gtctcggaat
     3301 aatggaacct cctcgtcctc tgacgtcagt gagggcaggg cagctgaagc atctcaggga
     3361 tttagatttc ttaggcgaag atgggggttg tcgtcgctca gccaaaatca tagctctgaa
     3421 ccagaggcag aaaattttaa ccaagaatca gaaggtagaa attcaggacc atggttgtct
     3481 tcttcactta gaaatagatg cacacctttg ttctcgagaa ggaggcgaga gggaagggat
     3541 gagtcttcaa gaatgtctac gtcagatgta ccacctagat ctcatatttt cagaagagat
     3601 tcaaatgaag tagttcatct tgaagcacag ggtgactccc ttggggctgc tgccaaccga
     3661 ccacaagcat ctggagcgtc aagcagtgct gctgcaggtg gctccacccc agagttgcct
     3721 cagggtggaa gaaatccagg actaacaggg attcttcctg gctccttgtt ccggtttgca
     3781 gtcccaccag cactcggcag taatctggct gacaatgtca tgattactgt agatattatt
     3841 ccttctggtt ggaattcaac tgatgggaaa aatgataaag ctaaaagtgc accttcaaga
     3901 gacccagaaa aacttcagaa aatcaaagaa agcctccttt tagaggactc tgatgatgaa
     3961 gaagaagggg acttatgtag aatttgtcag atggcagcag cgtcatcatc taatttattg
     4021 atagagccgt gcaaatgcac agggagcctg cagtacgtcc atcaagagtg tatgaaaaag
     4081 tggttacaag ccaaaattaa ttctggctct tcattagagg ctgtgactac ctgtgaactc
     4141 tgtaaagaga agttgcaact taacctggag gattttgata ttcatgaact acatagagct
     4201 catgcaaatg aacaagctga gtatgagttt atcagctctg gtctctacct agttgtctta
     4261 ctgcacttgt gtgaacaaag cttttctgat atgatgggaa atacaattga accaagcact
     4321 cgtgtccgat ttattaacct tgcaagaact cttcaggcac atatggaaga tctcgaaact
     4381 tcagaggatg aattcggatc cgactacaaa gaccatgacg gtgattataa agatcatgac
     4441 atcgactaca aggatgacga tgacaagtag cggccgcgaa ggatctgcga tcgctccggt
     4501 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     4561 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     4621 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     4681 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     4741 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     4801 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     4861 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     4921 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     4981 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg
     5041 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg
     5101 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg
     5161 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac
     5221 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc
     5281 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc
     5341 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc
     5401 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc
     5461 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc
     5521 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg
     5581 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc
     5641 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc
     5701 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac
     5761 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc
     5821 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc
     5881 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     5941 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6001 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6061 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6121 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6181 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6241 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6301 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6361 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6421 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga
     6481 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6541 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6601 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6661 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6721 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6781 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6841 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6901 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6961 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7021 tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct
     7081 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata
     7141 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7201 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7261 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7321 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7381 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     7441 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7501 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     7561 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7621 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7681 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat
     7741 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7801 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7861 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7921 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7981 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8041 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8101 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8161 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8221 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8281 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8341 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8401 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8461 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8521 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8581 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8641 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8701 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8761 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8821 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8881 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8941 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9001 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9061 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9121 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9181 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9241 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9301 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9361 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9421 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9481 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9541 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9601 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9661 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9721 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9781 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9841 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9901 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     9961 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
    10021 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    10081 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
    10141 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    10201 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
    10261 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
    10321 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg
//
