LOCUS       V029870                13498 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029870
VERSION     V029870
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..7524
                     /label="Marf1(NM_001081154)"
                     /note="Marf1(NM_001081154)"
                     /gene="Marf1"
     CDS             7531..7596
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        7640..7851
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7864..8132
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      8161..8170
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             8191..8853
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             8923..8976
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             8977..9576
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9577..10165
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             10239..10472
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10544..10665
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10705..10840
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10873..10889)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10897..10913
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10921..10951)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10966..10987
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(11275..11863)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12034..12894)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12895..12999)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13473..13489
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg aagggaaagg aactgagaac
     2341 ccttgcagca gaacacttgg atggtttcac caagataatg atgctaagcc gtggctttgg
     2401 aaattctctg gttgcttttc tcgtcccgag cagacattgc ctcttagttc ccaaacgaaa
     2461 gagtacatgg agaacaagaa agctgctgtg gagttaaagg atataccatc tccccttcat
     2521 gttggctcta aatttttccc agcagtccca cttcctgata ttcgttctct tcagcaacct
     2581 aaagtacagc tctcagctat tcccaaagta agctgctgtg ctcattgccc taatgaaccc
     2641 tccacttcac ccatgcgttt tggtggtggc agtggcagta gtggaggttc tgggagcttg
     2701 attcacacgg gctcactctt agactcacca agcactggga cagtcacgtg tcaagtaggg
     2761 tcaggttttg ctttccagtc cgtgtcttca ctgcagaatg cctccactag gaacaacttg
     2821 gtgggccttt caagtgactt ccccagcatg tgtgtagaga gtaacctacc ttcctgcaag
     2881 cacctgtcgt gttgtgggaa gctccatttc cagtcatgtc acagtaatgt acacaagctg
     2941 catcagttcc agaatcttca gggctgtgcc tctgctggct atttcccctg ttccgatttc
     3001 ccaagtgggg ctcctgggca tttggaagag cgcctttccc attcagagct tacacctcac
     3061 ttgtgcacca actctcttca cctaaatgtg gcacctccgg tttgtttaaa gggctcactt
     3121 tactgtgaag actgtctaaa caagccagca agaaatagta taattgatgc tgctaaaatc
     3181 tggccaaata taccacctcc aagtactcag cctgcacctc cagctattcc tgtgtgtaat
     3241 ggctgtggaa caaaaggcat ggaaaaggag acctcattgt tgttggcaac cagtctaggc
     3301 aaaactgctt cgaaatttgg gtcaccagaa gttgcagtca ctggacaggt gctggagacc
     3361 ctccccccca ttggcgtttt ctgggatatt gaaaactgct cagtcccctc tggccggtca
     3421 gcaacaactg ttgtacagag aatccgtgag aagtttttta gaggacacag agaagcagag
     3481 tttatctgtg tgtgtgacat cagtaaagaa aacaaggaag ttattcaaga gctgaataat
     3541 tgtcaggtaa ccgttgctca cataaatgct actgcaaaga atgctgctga tgataaactc
     3601 cgtcagagcc tgcgaaggtt cgcaaacacg cacactgctc cagctactgt ggttcttgtg
     3661 tcaactgatg tcaattttgc gttggaactc agtgatctta ggcaccggca cggtttccac
     3721 atcattttgg tacataaaaa ccaggcctct gaagccttgt tacatcatgc taaccagctg
     3781 atcagatttg aagagttcat ttctgatctc ccccctcggt taccactgaa gattccttgc
     3841 cacactctac tctatgttta taacctgcca gcaaataaag atggcaagag catcagcaac
     3901 aggctcaggc gcctctctga taactgtggt ggcaaggtgc tgagtatcac aggctgcagc
     3961 gcaattctcc gcttcataaa ccaggacagt gcagagcggg cccagaagcg aatggaaaat
     4021 gaagatgtct ttggcaacag gatcatcgtg tcctttactc caaaacatag agaattcttt
     4081 gaagccaaga gttcaaatgc tattgccgac aaagtgaagt ctcccaaaaa agttaagaac
     4141 acaaaattgt gcctcatcaa agacacaagt gaacagtcac ccagtgtcaa agccatgcca
     4201 gggaaagtgt tgcaggcaaa ttctggatct gctacaaaaa ccacaaacat taaaagttta
     4261 caggagctgt gccgaatgga gtcaaagtct ggcaatagaa acagtgacca tcagcaaggc
     4321 catgggaggc tggcagcact gcctaacagt ggtccaactg cttcagtgcc catcgtgaag
     4381 aacacaggtg tgacagagcc actttacaga agcagtcaga aaaaagagaa ccccagttcc
     4441 cagagcactg tcaactctcc tgtagagaag aagaagagag aggagactgt cttccaagta
     4501 agctacccat ccgcattcag caagctgatc gcctcaaggc aagtcagccc tctgctcacc
     4561 tctcagtctt ggtctccgag gagtatgtct ccaaaccttt tgaatagagc ctcaccactt
     4621 gctttcaaca ttgccaatcc cagcagcggt gctgactgcc cagacccatt tgcaaatggt
     4681 gttgacatcc aggtcagcaa tgtggactac aggctgtccc ggaaggagct gcagcagctc
     4741 ctccaggaag ccttttccaa gcatggccag gtgaagagtg ttgagctcag tccccatact
     4801 gattaccagc tcaaggccat tgtgcagatg agaaacctgc acgaagcaat ctgtgcagtg
     4861 aacagcctcc ataggcacaa aattggcagc aagaagatac tggtctcact ctccacagga
     4921 gctgccaaca agtcactctc tttactaagc acagaaacaa tgtctattct tcaggatgct
     4981 cctgcctgtt gcttgcctct ctttaaattt atagacatct atgaaaagaa gtatggacac
     5041 aagttaaatg tgtctgattt atataaatta acagacacta ttgcaatccg tgaacaggga
     5101 aacgggcgat tggtgtgtct cttacctagt aatcaggccc gccagagccc cctgggctct
     5161 tcccagtcac atgatggctc ctcctcaaac tgcagcccag ttctgtttga agagttggag
     5221 tatcacgaac ctgtctgcaa gcagcactgc tctaacaagg acttcagtga acttgtgttt
     5281 gatccagact cttacaagat tccttttgtg gttctctctt tgaaagtgtt cgcacctcaa
     5341 gttcatagtc ttctgcagac ccaccagggc actgtgcctt tgctaagctt tccagattgc
     5401 tatgctgcgg agtttggtga cctagaaatt acgcaagaca gccacaaggg tgtcccctta
     5461 gagcacttca tcacctgcat tccaggagtg aacattgcca ctgctcagaa tggcgtcaaa
     5521 gtagtgaagt ggattcacaa caagccccca cctcccaaca ctgacccttg gcttcttcgt
     5581 tctaaaagtc ctgtgagaaa tccccagctg attcagttca gcagagaggt cattgattta
     5641 ctgaagaccc agccatcgtg tatactaccc gtcagcaatt tcatcccttc ataccatcac
     5701 cactttggaa agcagtgcag ggtttcagac tatggttatt ccaagctgat tgaactccta
     5761 gaagctgtgc ctcatgtgtt gcagattctt ggtatgggct ccaagcgtct gatgaccctt
     5821 actcacaggg cccaggtgaa gcgctttact caggatttac taaaacttct taagtcccag
     5881 gccagcaagc aggtcatcgt gagagacttt tcacaggctt atcattggtg tttctcaaag
     5941 gactgggatg tcacggaata tggtgtttgt gacttgattg atataatatc agagatccca
     6001 gacacaacta tttgcttgtc ccaacaagat gacgacatgg tcatttgtat ccgaaaaaga
     6061 gaacgaactc aggatgaaat agaaaggaca aagcagttct ccaaagatgt tgtggacttg
     6121 ctgcgccacc agccccattt cagaatgccc ttcaacaagt tcattccctc ttaccaccac
     6181 cactttggcc gacagtgtaa acttgcatac tatggattta ccaagctgct tgaacttttg
     6241 gaagccatac ctgagatttt acaagtgttg gaatgtggag aagaaaaaat cctgactttg
     6301 actgaagtgg agcgattcaa agctctagcc gcccagtttg ttaagcttct gcggtcccaa
     6361 aagggtaact gccttatgat gacagatctg tttacagaat atgctaaaac ttttggttat
     6421 acatttcgtc tccaagacta tgatgtcagt tcagtctcag ctttgacaca gaagctctgc
     6481 catgttgtaa aggttgctga tatggagtct ggtaaacaga tccagctgat caataggaag
     6541 tctctgcggt ctctcactgc tcagttgctg gtcttgttga tgtcttggga aggggatgct
     6601 tatcttcctg ttgatgagct caggagacat tatgaaacca ctcacagcac tcctctcaac
     6661 ccctgtgaat atggattcat gaccctgact gagctactga agagtctgcc gtacttagta
     6721 gaggtttgta cgaacgataa ggcagaagag tatgtgaagc tcacaagcct gtacttgttt
     6781 gcaaagaatg tgaggtcttt gcttcatacc tatcactacc agcagatttt tcttcatgaa
     6841 ttttccatgg cctataacaa atatgttgga gaaacactac agcccaaaac ttatggctac
     6901 agtagtgtgg aggaattact gggagccatc ccacaggtgg tctggataaa aggacatggt
     6961 cataagagaa ttgtagtgtt aaaaaatgac atgaaaaacc gcctaagctc atttgacctt
     7021 ttctctgtca atcatgaaga ccagcctgca gtgagcaggc agatcctggc ggtgccagaa
     7081 tcccatctag cctcagaact ccagctccga acaggaccca gccagatgga gcaggagctt
     7141 ctgcacctgg ccagcagctc ccccgttgac ctgctctgtg ctcctgttcc ttcatgcctg
     7201 ccatcccctc agctaagacc agatcctgtc atcctgaagc ccgctgatct cattcagttt
     7261 gaggaacacc ctcaagagcc tttgggagtt ttagttttaa accaagaaga aaaatcagaa
     7321 gttccactac cagtgcaaaa gggaaacctg tcctgtgact cctcacccag cagcccagca
     7381 gcttctcctg ctcctcctgg cccctcctca gaagccccca ggccactttt cagcaaggat
     7441 gcagtggaaa gcccagccaa aaagcagccc aaaaatagag tcaaattggc agccaacttt
     7501 tcttttgcac ctgtaaccaa gcttggatcc gactacaaag accatgacgg tgattataaa
     7561 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     7621 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     7681 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     7741 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     7801 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     7861 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     7921 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     7981 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     8041 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     8101 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     8161 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     8221 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     8281 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     8341 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     8401 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     8461 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     8521 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     8581 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     8641 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     8701 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     8761 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     8821 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     8881 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     8941 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     9001 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     9061 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     9121 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     9181 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     9241 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     9301 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     9361 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     9421 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     9481 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     9541 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     9601 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     9661 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     9721 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     9781 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     9841 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     9901 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     9961 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
    10021 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
    10081 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
    10141 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
    10201 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
    10261 acgaagataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
    10321 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    10381 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    10441 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
    10501 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
    10561 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10621 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
    10681 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    10741 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    10801 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa
    10861 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    10921 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    10981 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    11041 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    11101 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    11161 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    11221 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    11281 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    11341 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    11401 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    11461 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11521 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11581 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    11641 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    11701 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    11761 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    11821 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    11881 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    11941 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    12001 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    12061 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    12121 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    12181 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    12241 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    12301 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    12361 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    12421 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    12481 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    12541 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    12601 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    12661 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    12721 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    12781 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    12841 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    12901 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    12961 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    13021 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    13081 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    13141 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    13201 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
    13261 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    13321 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    13381 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    13441 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
