LOCUS       V021967                10366 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021967
VERSION     V021967
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..4408
                     /label="Mybl2(NM_008652)"
                     /note="Mybl2(NM_008652)"
                     /gene="Mybl2"
     CDS             4415..4480
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4525..4736
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4749..5017
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5046..5055
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5076..5738
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5808..5861
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5862..6461
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6462..7049
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7123..7356
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7428..7549
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7568..7703
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7741..7757)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7765..7781
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7789..7819)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7834..7855
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8143..8731)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8902..9762)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9763..9867)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10341..10357
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgt ctcggcggac gcgctgcgag gatctggatg agttacacta
     2341 ccaggacgtg gactcagacc tcctggagca gagagacaac agatgtaagg ttaaatggac
     2401 ccacgaggag gacgagcagt tgagggccct ggtgaggcag tttggacagc aagactggaa
     2461 gttcttggcc agtcactttc ctaaccgcac agaccagcaa tgccagtacc ggtggctgag
     2521 ggttttgaat cccgaccttg ttaagggacc atggaccaaa gaggaagatc aaaaggtcat
     2581 tgagttggtc aagaagtatg gcaccaaaca gtggacgctg atagccaagc acctgaaggg
     2641 ccggctgggg aagcagtgcc gtgagcgctg gcacaaccac ctcaaccccg aggtgaagaa
     2701 gtcatgctgg accgaggagg aagacaggat aatctgtgag gcccataaag tcctgggtaa
     2761 ccgctgggct gagatcgcca agatgctacc agggaggacg gacaatgctg tgaagaatca
     2821 ctggaactcc actatcaaaa ggaaagtcga cacgggaggt ttcccagccg agtccaggga
     2881 ctgcaagcct gtctacttgc tcctggagct ggaggacaag gaacagcacc agggtgtcca
     2941 gccggtggac ggccagggaa gtctcgtgag cagctggccg ctggtgccct ctattgtgaa
     3001 ggaggagagc agcgaggagg agattgccat agctgctact tctgctaaag aactcggaca
     3061 tgagcctgtc cctgctgatc tgggagaagt gcgcacccca gagcccccag aatctctcaa
     3121 gcgtgaatac caggagttct cctccccgga aacgagcctg ccctacaagt gggtggtaga
     3181 ggcggcaaac ctcctcatcc cggctgtggg gtccagcctc tctgaagctc tggacttgat
     3241 tgagtcggac cctgatgctt ggtgcgacct gagtaaattt gaccttcctg aagaaccctc
     3301 tacggagggc agtgtcgtca gcagcccagt gcagccccag acgtcgcagc agcagcagga
     3361 ggaggcactg cagtcatccc agcaggctgc cacgccgggg cccagtgtga ctgagtaccg
     3421 ccttgatggc cacactattt cagacctgag ccggagcagt cggggggagc tgatccccat
     3481 ttctcctagc actgagtttg ggggctcagg cattggcaca cctccctcag tgctcaagcg
     3541 acagaagaaa cggcgtgtgg ccctgtcgcc tgtcacagag aacagtgcca gcctgtcctt
     3601 cctggactct tgtaacagcc tcaccccgaa gagcacgcct gtcaaaaccc tccccttctc
     3661 tccttcccag tttctgaact tctggaacaa acaggatacc ctggagctgg agagcccctc
     3721 actgacatcc actccagtgt gtagccaaaa ggtggtcgtc accacgcccc tgcacaggga
     3781 taagacaccc ctgcaccaga agtatccatc atcagaagta ctcccagatc agaagtactc
     3841 catggacaac actccccaca cgccaacccc gttcaagaat gccctggaga agtacggacc
     3901 gctgaagccc ctgccgcaga ctccgcacct ggaggaggac ttgaaggagg tgctccgttc
     3961 tgaggccggc atggagctca tcattgagga tgacatgagg cccgagaagc agaagagaaa
     4021 gcctgggctg cggcgaagcc ctatcaagaa ggtccgcaag tctctggctc tcgacattat
     4081 ggatgaagat gggaagctga tgtcctctac catgcccaaa cccctgtcct tgccaaccag
     4141 tgtcacaccc agctcctgtg gcttcacctc gccaggtagc aaagagggta acagcctgct
     4201 caaccagggt ttcctacagg ccaaacccga gaaggtggtg gccgcccaga agactcggag
     4261 ccacattcca acccctgccc ctatgaccca tgcctggaag acggtggcct gtgggggcac
     4321 caaagaccag ctcttcatgc aggagaaagc ccggcagctc ctgagccgcc tgaagtccag
     4381 ccacacatct cggaccctca ttctgtccgg atccgactac aaagaccatg acggtgatta
     4441 taaagatcat gacatcgact acaaggatga cgatgacaag tgagcggccg cgaaggatct
     4501 gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     4561 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
     4621 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     4681 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga
     4741 agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc
     4801 cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg
     4861 ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga
     4921 gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg
     4981 tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc
     5041 tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca
     5101 tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca
     5161 agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct
     5221 acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct
     5281 acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga
     5341 agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg
     5401 tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg
     5461 acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc
     5521 tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg
     5581 tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc
     5641 ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg
     5701 agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct cagtcgtcca
     5761 attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag ggcagaggaa
     5821 gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag tacaagccca
     5881 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     5941 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     6001 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     6061 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     6121 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     6181 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     6241 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     6301 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     6361 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     6421 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct ctggattaca
     6481 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6541 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6601 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6661 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6721 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6781 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6841 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     6901 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6961 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga
     7021 gtcggatctc cctttggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg
     7081 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     7141 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     7201 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7261 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7321 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     7381 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     7441 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7501 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     7561 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7621 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7681 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg
     7741 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7801 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7861 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7921 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7981 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8041 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8101 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8161 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8221 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8281 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8341 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8401 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8461 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8521 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8581 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8641 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8701 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8761 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8821 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8881 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8941 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9001 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9061 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9121 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9181 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9241 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9301 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9361 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9421 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9481 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9541 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9601 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9661 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9721 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9781 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9841 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     9901 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     9961 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    10021 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    10081 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    10141 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    10201 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    10261 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    10321 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
