LOCUS       V046851                11698 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046851
VERSION     V046851
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..5724
                     /label="Myo1b(NM_001161817)"
                     /note="Myo1b(NM_001161817)"
                     /gene="Myo1b"
     CDS             5731..5796
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5840..6051
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6064..6332
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6361..6370
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6391..7053
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             7123..7176
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7177..7776
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7777..8365
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8439..8672
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8744..8865
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8905..9040
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9073..9089)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9097..9113
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9121..9151)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9166..9187
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9475..10063)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10234..11094)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11095..11199)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11673..11689
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg ccaaaatgga ggtgaaatcc
     2341 tcacttctgg acaatatgat cggtgttgga gatatggttc ttttagagcc gctcaatgag
     2401 gagacgttca tcgacaacct gaagaagcgc ttcgaccaca atgagatata tacatacatt
     2461 gggagcgtgg ttatatctgt taacccctac cggtccttgc ccatatattc gccggagaaa
     2521 gtggaagatt acagaaatag aaacttctat gaactgagcc ctcacatctt tgctttgtcg
     2581 gatgaagcat acagatccct ccgtgatcaa gataaagatc aatgcattct cattactggg
     2641 gagagtggag caggaaaaac agaggccagc aagctcgtca tgtcctatgt cgctgccgtg
     2701 tgcggaaaag gagcagaagt caatcaggtc aaggaacagc ttctgcagtc caaccctgtc
     2761 ctggaagctt ttggaaatgc caagactgtg aggaatgaca actcatctag attcggcaaa
     2821 tacatggata ttgaatttga ctttaaaggc gatccgctgg gaggagtgat aagtaactat
     2881 cttttagaga aatctcgtgt tgttaagcag ccaagaggtg aaagaaactt ccatgtgttc
     2941 tatcagctgc tctcaggcgc ctctgaggag ctcctctaca agcttaagct ggagcgggat
     3001 ttcagcagat acaactatct gagtctggac tctgctaaag ttaatggtgt ggatgatgct
     3061 gccaatttca gaactgttag gaatgcgatg cagatcgtgg gctttctgga tcatgaggcc
     3121 gaggctgtcc tggaggtagt ggcagccgtt ttgaaactgg ggaacatcga gttcaagcct
     3181 gaatcacgag tgaatggttt ggatgaaagc aaaatcaaag ataaaaatga gttaaaagaa
     3241 atttgcgagt tgaccagcat cgatcaggtg gttttagaaa gagcgttcag tttccgaaca
     3301 gttgaagcga agcaggagaa agtttcaacc acgctgaatg tggctcaggc ttattatgcc
     3361 cgtgatgccc tggctaaaaa cctctacagc cgcttgtttt catggttggt aaatcgtatc
     3421 aacgaaagca ttaaggcaca aacaaaagtg agaaagaagg tcatgggtgt tctggacatt
     3481 tatggctttg aaatatttga ggataacagc ttcgagcagt ttattattaa ctattgtaat
     3541 gaaaagcttc aacaaatctt catcgaactt accctcaaag aagagcaaga ggaatatatt
     3601 cgggaggaca tagaatggac tcacattgac tacttcaaca atgctatcat ttgtgaccta
     3661 atagaaaata acacaaatgg aatcttggcc atgctggatg aagagtgcct gagacccggc
     3721 actgttacag atgagacctt cctggaaaag ctgaaccaag tctgtgccac ccaccagcac
     3781 tttgagagca ggatgagcaa gtgttcccgc tttctcaacg acacaaccct gccccacagc
     3841 tgcttcagaa tccagcatta tgctggcaag gtgctctacc aggtggaagg atttgttgac
     3901 aagaacaatg accttctgta ccgggacctg tctcaagcca tgtggaaggc cggccactcc
     3961 ctcatcaagt ctctgtttcc tgaaggaaat cctgcaaagg tcaacctgaa gagacctccc
     4021 accgcaggct cccagttcaa ggcgtctgtg gccacgctga tgagaaacct gcagaccaag
     4081 aacccgaact acatcaggtg catcaagcca aatgataaaa aggcagctca cattttcaat
     4141 gagagtctcg tatgccatca gatcaggtac ctgggtcttt tggagaacgt tcgagtgaga
     4201 cgggcaggct atgccttcag gcaggcctat gaaccttgcc tggaaagata caagatgctt
     4261 tgcaaacaaa cgtggcctca ctggaaggga ccagcaaggt ctggcgtgga ggttctgttt
     4321 aatgagctag agattcctgt ggaagagcac tccttcggga gatccaagat attcatccga
     4381 aacccacgga cattattcca actagaagac ctaaggaagc agcggctgga ggacctggcc
     4441 actctcattc agaagattta tcgaggatgg aaatgccgta cacattttct gctcatgaaa
     4501 agaagccaag ttgtgattgc tgcctggtac aggagatatg cgcaacaaaa gcggtaccag
     4561 cagataaaga gttcagccct ggtgattcag tcgtacatcc ggggctggaa ggctcgaaaa
     4621 atcctgcggg agctgaagca tcagaagcgc tgtaaggagg cagccaccac catcgcggcg
     4681 tactggcatg gcacccaggc gcgaagggag ctgagacggc tgaaggacga ggctaggaat
     4741 aagcacgcta ttgcagttat ttgggcttac tggcttggat cgaaggcacg gagggagttg
     4801 aaacgcttga aggaggaggc taggcgtaag catgcagttg ctgtcatttg ggcttactgg
     4861 cttggactga aggtacgcag ggaatacagg aaattcttta gagcaaatgc tggaaagaaa
     4921 atctatgagt ttacacttca aagaattgtg caaaaatact tgttggaaat gaaaaataaa
     4981 atgccttcct tgtcaccgat agataaaaat tggccatcaa gaccttacct gttcttggat
     5041 tcgactcaca aggagctaaa aaggatcttc cacttgtgga ggtgtaaaaa atacagggat
     5101 caattcacag accagcagaa acttatttat gaagagaagc tcgaggccag cgaactcttc
     5161 aaagacaaga aggctttata tccttctagt gttgggcaac cattccaagg agcttacctg
     5221 gaaatcaaca agaacccaaa atataagaaa ctcaaagatg ccattgaaga gaagatcatt
     5281 attgctgagg ttgtgaacaa aattaatcgg gctaatggga agagtacatc tcggattttc
     5341 ctcttaacaa acaataacct tctccttgct gaccaaaagt ctgggcagat caagtccgag
     5401 gtccccctgg tagatgtgac caaggtttcc atgagttccc agaacgatgg tttctttgca
     5461 gtgcacctca aggagggttc agaagcggct agtaaagggg actttctctt cagcagtgac
     5521 cacctgattg aaatggcaac caagctgtat cgcacgactc tcagccaaac taaacagaag
     5581 ctcaacatcg agatttccga tgagttcctg gtacagttca gacaggacaa agtatgtgtg
     5641 aagtttattc aaggcaacca gaaaaatggg agcgtgccaa cctgcaaacg aaagaacaac
     5701 agactccttg aagtcgctgt ccctggatcc gactacaaag accatgacgg tgattataaa
     5761 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     5821 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     5881 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     5941 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     6001 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     6061 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     6121 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     6181 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     6241 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     6301 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     6361 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     6421 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     6481 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     6541 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     6601 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     6661 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     6721 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     6781 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     6841 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     6901 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     6961 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     7021 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     7081 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     7141 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     7201 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     7261 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     7321 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     7381 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     7441 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     7501 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     7561 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     7621 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     7681 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     7741 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     7801 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7861 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7921 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7981 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8041 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8101 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     8161 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     8221 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     8281 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     8341 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     8401 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     8461 acgaagataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     8521 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8581 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8641 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     8701 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     8761 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8821 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     8881 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8941 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     9001 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa
     9061 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     9121 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     9181 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9241 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9301 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9361 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9421 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9481 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9541 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9601 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9661 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9721 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9781 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9841 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9901 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9961 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10021 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10081 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    10141 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    10201 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10261 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10321 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10381 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10441 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10501 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10561 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10621 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10681 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10741 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10801 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10861 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10921 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10981 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    11041 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    11101 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    11161 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11221 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    11281 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    11341 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    11401 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
    11461 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    11521 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    11581 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    11641 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
