LOCUS       V047638                11239 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047638
VERSION     V047638
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3018
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             3049..6093
                     /label="NCAPG(NM_022346)"
                     /note="NCAPG(NM_022346)"
                     /gene="NCAPG"
     CDS             6100..6165
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        6209..6420
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6433..6701
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6728..7327
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7334..7922
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7996..8229
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8301..8422
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8441..8576
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8614..8630)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8638..8654
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8662..8692)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8707..8728
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9016..9604)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9775..10635)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10636..10740)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11214..11230
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2341 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2401 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2461 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2521 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2581 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2641 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2701 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2761 atggccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag
     2821 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2881 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2941 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3001 atggacgagc tgtacaaggg tggaggcgga tcaggaggcg gtggatctat gggagcggaa
     3061 aggaggctgc tgtcgattaa ggaggccttt cggctggcgc agcagccgca ccagaaccag
     3121 gcgaagctgg tggtggcgct gagccgcacc taccgcacga tggatgataa gacagttttt
     3181 catgaggagt tcattcatta ccttaaatat gttatggtgg tctataaacg tgaaccagct
     3241 gtggagaggg taatagaatt tgcagcaaag tttgttacct catttcacca atcagatatg
     3301 gaagatgatg aggaagagga agatggtggc cttttaaatt atttgtttac ttttctctta
     3361 aagtctcatg aagcaaacag caatgcagtg agatttagag tgtgcctgct cataaacaag
     3421 cttttgggaa gtatgccaga aaatgctcag attgatgatg atgtgtttga taaaattaat
     3481 aaagccatgc ttattagatt gaaagataag attccaaatg tgagaataca ggcagttctg
     3541 gcgctttcac gacttcagga tcccaaggat gatgaatgcc cagtggttaa tgcatatgct
     3601 actttgattg aaaatgattc aaatccagaa gttagacggg cagtgttatc atgtattgca
     3661 ccatcagcaa agactttgcc aaaaattgta gggcgcacca aggatgtgaa agaggctgtc
     3721 agaaagctgg cttatcaggt tttagctgaa aaggttcata tgagagctat gtccattgct
     3781 cagagagtaa tgctccttca acaaggtctt aatgacagat cagatgctgt gaaacaagct
     3841 atgcagaagc atcttcttca aggctggtta cggttctctg aaggaaatat cttagagttg
     3901 ctccatcggt tggatgtaga aaattcttct gaagtggcag tctctgttct caatgccttg
     3961 ttttcaataa ctcctctcag tgaactggtg ggactctgta aaaacaatga tggcaggaaa
     4021 ttgattccag tggaaacatt aactcctgaa attgctttgt attggtgtgc cctttgtgaa
     4081 tatttgaaat caaaaggaga tgaaggtgaa gaatttttag agcagatttt gccagagcct
     4141 gtagtatatg cagactattt attgagttac atccagagca ttccagttgt taatgaagaa
     4201 cacagaggtg atttttccta tattggaaat ttgatgacaa aagaattcat aggtcaacaa
     4261 ttgattctaa ttattaagtc tttggatacc agtgaagaag gaggaagaaa aaaactgctg
     4321 gctgttttac aggagattct tattttaccc acaatcccaa tatccctggt ttcttttctt
     4381 gttgaaagac tactccacat cattatagat gataataaga gaacacaaat tgttacagaa
     4441 attatctcag agattcgggc gcccattgtt actgttggtg ttaataacga tccagctgat
     4501 gtaagaaaga aagaactcaa gatggctgaa ataaaagtta agcttatcga agccaaagaa
     4561 gctttggaaa attgcattac cttacaggat tttaatcggg catcagaatt aaaagaagaa
     4621 ataaaagcat tagaagatgc cagaataaac cttttgaaag agacagagca acttgaaatt
     4681 aaagaagtcc acatagagaa gaatgatgct gaaacattgc agaaatgtct tattttatgc
     4741 tatgaactgt tgaagcagat gtccatttca acaggcttaa gtgcaaccat gaatggaatc
     4801 atcgaatctt tgattcttcc tggaataata agtattcatc ctgttgtaag aaacctggct
     4861 gttttatgct tgggatgctg tggactacag aatcaggatt ttgcaaggaa acacttcgta
     4921 ttactattgc aggttttgca aattgatgat gtcacaataa aaataagtgc tttaaaggca
     4981 atctttgacc aactgatgac gttcgggatt gaaccattta aaactaaaaa aatcaaaaca
     5041 cttcattgtg aaggtacaga aataaacagt gatgatgagc aagaatcaaa agaagttgaa
     5101 gagactgcta cagctaagaa tgttctgaaa ctcctttctg atttcttaga tagtgaggta
     5161 tctgaactta ggactggagc tgcagaagga ctagccaagc tgatgttctc tgggcttttg
     5221 gtcagcagca ggattctttc tcgtcttatt ttgttatggt acaatcctgt gactgaagag
     5281 gatgttcaac ttcgacattg cctaggcgtg ttcttccccg tgtttgctta tgcaagcagg
     5341 actaatcagg aatgctttga agaagctttt cttccaaccc tgcaaacact ggccaatgcc
     5401 cctgcatctt ctcctttagc tgaaattgat atcacaaatg ttgctgagtt acttgtagat
     5461 ttgacaagac caagtggatt aaatcctcag gccaagactt cccaagatta tcaggcctta
     5521 acagtacatg acaatttggc tatgaaaatt tgcaatgaga tcttaacaag tccgtgctcg
     5581 ccagaaattc gagtctatac aaaagccttg agttctttag aactcagtag ccatcttgca
     5641 aaagatcttc tggttctatt gaatgagatt ctggagcaag taaaagatag gacatgtctg
     5701 agagctttgg agaaaatcaa gattcagtta gaaaaaggaa ataaagaatt tggtgaccaa
     5761 gctgaagcag cacaggatgc caccttgact acaactactt tccaaaatga agatgaaaag
     5821 aataaagaag tatatatgac tccactcagg ggtgtaaaag caacccaagc atcaaagtct
     5881 actcagctaa agactaacag aggacagaga aaagtgacag tttcagctag gacgaacagg
     5941 aggtgtcaga ctgctgaagc cgactctgaa agtgatcatg aagttccaga accagaatca
     6001 gaaatgaaga tgagactacc aagacgagcc aaaaccgcag cactagaaaa aagtaaactt
     6061 aaccttgccc aatttctcaa tgaagatcta agtggatccg actacaaaga ccatgacggt
     6121 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     6181 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     6241 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     6301 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     6361 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     6421 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     6481 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     6541 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     6601 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     6661 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     6721 acgctagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     6781 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6841 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6901 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6961 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     7021 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     7081 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     7141 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     7201 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     7261 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     7321 tgcctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     7381 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     7441 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7501 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7561 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7621 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7681 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     7741 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7801 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7861 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7921 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7981 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg
     8041 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8101 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8161 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8221 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     8281 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     8341 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8401 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     8461 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8521 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga
     8581 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8641 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8701 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8761 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8821 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8881 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8941 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9001 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9061 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9181 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9421 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9541 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9601 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9661 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9721 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9781 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9841 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9901 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9961 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10021 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10081 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10141 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10201 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10261 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10321 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10381 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10441 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10501 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10561 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10621 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10681 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10741 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10801 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    10861 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10921 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    10981 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    11041 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    11101 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    11161 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    11221 gacggccagt gccaagctg
//
