LOCUS       V034081                10428 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034081
VERSION     V034081
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5274
                     /label="NEK9(NM_001329237)"
                     /note="NEK9(NM_001329237)"
                     /gene="NEK9"
     CDS             5281..5346
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5390..5601
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5614..5882
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5917..6516
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6523..7111
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7185..7418
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7490..7611
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7630..7765
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7803..7819)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7827..7843
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7851..7881)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7896..7917
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8205..8793)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8964..9824)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9825..9929)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10403..10419
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgtcggtg ctgggcgagt acgagcgaca ctgcgattcc
     2341 atcaactcgg actttgggag cgagtccggg ggttgcgggg actcgagtcc ggggcctagc
     2401 gccagtcagg ggccgcgagc cggcggcggc gcggcggagc aggaggaact gcactacatc
     2461 cccatccgcg tcctgggccg cggcgccttc ggggaagcca cgctgtaccg ccgcaccgag
     2521 gatgactcac tggttgtgtg gaaggaagtc gatttgaccc ggctgtctga gaaggaacgt
     2581 cgtgatgcct tgaatgagat tgttattctg gcactgctgc agcacgacaa cattattgcc
     2641 tactacaatc acttcatgga caataccacg ctgctgattg agctggaata ttgtaatgga
     2701 gggaacctgt atgacaaaat ccttcgtcag aaggacaagt tgtttgagga agagatggtg
     2761 gtgtggtacc tatttcagat tgtttcagca gtgagctgca tccataaagc tggaatcctt
     2821 catagagata taaagacatt aaatattttt ctgaccaagg caaacctgat aaaacttgga
     2881 gattatggcc tagcaaagaa acttaattct gagtattcca tggctgagac gcttgtggga
     2941 accccatatt acatgtctcc agagctctgt caaggagtaa agtacaattt caagtctgat
     3001 atctgggcag ttggctgcgt catttttgaa ctgcttacct taaagaggac gtttgatgct
     3061 acaaacccac ttaacctgtg tgtgaagatc gtgcaaggaa ttcgggccat ggaagttgac
     3121 tctagccagt actctttgga attgatccaa atggttcatt cgtgccttga ccaggatcct
     3181 gagcagagac ctactgcaga tgaacttcta gatcgccctc ttctcaggaa acgcaggaga
     3241 gagatggagg aaaaagtcac tctgcttaat gcacctacaa agagaccaag gtcaagcact
     3301 gtgactgaag cacccattgc tgtagtaaca tcacgaacca gtgaagtcta tgtttggggt
     3361 ggtggaaaat ccacccccca gaaactggat gttatcaaga gtggctgtag tgcccggcag
     3421 gtctgtgcag ggaataccca ctttgctgtg gtcacagtgg agaaggaact gtacacttgg
     3481 gtgaacatgc aaggaggcac taaactccat ggtcagctgg gccatggaga caaagcctcc
     3541 tatcgacagc caaagcatgt ggaaaagttg caaggcaaag ctatccgtca ggtgtcatgt
     3601 ggtgatgatt tcactgtctg tgtgactgat gagggtcagc tctatgcctt cggatcagat
     3661 tattatggct gcatgggggt ggacaaagtt gctggccctg aagtgctaga acccatgcag
     3721 ctgaacttct tcctcagcaa tccagtggag caggtctcct gtggagataa tcatgtggtg
     3781 gttctgacac gaaacaagga agtctattct tggggctgtg gcgaatatgg taggggtagc
     3841 cttgtctctg tccacttctt cacaggacga ctgggtttgg attcagaaga ggattattat
     3901 acaccacaaa aggtggatgt tcccaaggcc ttgattattg ttgcagttca atgtggctgt
     3961 gatgggacat ttctgttgac ccagtcaggc aaagtgctgg cctgtggact caatgaattc
     4021 aataagctgg gtctgaatca gtgcatgtcg ggaattatca accatgaagc ataccatgaa
     4081 gttccctaca caacgtcctt taccttggcc aaacagttgt ccttttataa gatccgtacc
     4141 attgccccag gcaagactca cacagctgct attgatgagc gaggccggct gctgaccttt
     4201 ggctgcaaca agtgtgggca gctgggcgtt gggaactaca agaagcgtct gggaatcaac
     4261 ctgttggggg gaccccttgg tgggaagcaa gtgatcaggg tctcctgcgg tgatgagttt
     4321 accattgctg ccactgatga taatcacatt tttgcctggg gcaatggtgg taatggccgc
     4381 ctggcaatga cccccacaga gagaccacat ggctctgata tctgtacctc atggcctcgg
     4441 cctatttttg gatctctgca tcatgtcccg gacctgtctt gccgtggatg gcataccatt
     4501 ctcatcgttg agaaagtatt gaattctaag accatccgtt ccaatagcag tggcttatcc
     4561 attggaactg tgtttcagag ctctagcccg ggaggaggcg gcgggggcgg cggtggtgaa
     4621 gaagaggaca gtcagcagga atctgaaact cctgacccaa gtggaggctt ccgaggaaca
     4681 atggaagcag accgaggaat ggaaggttta atcagtccca cagaggccat ggggaacagt
     4741 aatggggcca gcagctcctg tcctggctgg cttcgaaagg agctggaaaa tgcagaattt
     4801 atccccatgc ctgacagccc atctcctctc agtgcagcgt tttcagaatc tgagaaagat
     4861 accctgccct atgaagagct gcaaggactc aaagtggcct ctgaagctcc tttggaacac
     4921 aaaccccaag tagaagcctc gtcacctcgg ctgaatcctg cagtaacctg tgctgggaag
     4981 ggaacaccac tgactcctcc tgcgtgtgcg tgcagctctc tgcaggtgga ggttgagaga
     5041 ttgcagggtc tggtgttaaa gtgtctggct gaacaacaga agctacagca agaaaacctc
     5101 cagattttta cccaactgca gaagttgaac aagaaattag aaggagggca gcaggtgggg
     5161 atgcattcca aaggaactca gacagcaaag gaagagatgg aaatggatcc aaagcctgac
     5221 ttagattcag attcctggtg cctcctggga acagactcct gtagacccag cctcggatcc
     5281 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     5341 gacaagtgag cggccgcaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc
     5401 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag
     5461 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     5521 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     5581 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc
     5641 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc
     5701 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg
     5761 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc
     5821 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga
     5881 tccaagctgt gaccggcgcc taccccgggc ggccctatga ccgagtacaa gcccacggtg
     5941 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     6001 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     6061 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     6121 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     6181 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     6241 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6301 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6361 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6421 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     6481 cgcacctggt gcatgacccg caagcccggt gcctgagtcg acaatcaacc tctggattac
     6541 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6601 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6661 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6721 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6781 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6841 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6901 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     6961 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7021 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7081 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     7141 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7201 ctcccaacga aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7261 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7321 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7381 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7441 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     7501 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7561 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7621 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7681 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7741 tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca
     7801 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7861 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7921 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7981 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8041 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8101 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8161 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8221 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8281 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8341 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8401 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8461 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8521 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8581 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8641 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8701 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8761 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8821 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8881 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8941 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9001 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9061 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9121 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9181 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9241 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9301 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9361 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9421 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9481 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9541 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9601 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9661 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9721 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9781 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9841 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9901 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     9961 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    10021 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
    10081 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
    10141 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
    10201 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
    10261 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    10321 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
    10381 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
