LOCUS       V035683                10438 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035683
VERSION     V035683
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4464
                     /label="NFATC1(NM_172390)"
                     /note="NFATC1(NM_172390)"
                     /gene="NFATC1"
     CDS             4471..4536
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4580..4791
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4804..5072
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5101..5110
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5131..5793
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             5863..5916
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5917..6516
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6517..7105
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7179..7412
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7484..7605
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7645..7780
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7813..7829)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7837..7853
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7861..7891)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7906..7927
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8215..8803)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8974..9834)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9835..9939)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10413..10429
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgc caagcaccag ctttccagtc
     2341 ccttccaagt ttccacttgg ccctgcggct gcggtcttcg ggagaggaga aactttgggg
     2401 cccgcgccgc gcgccggcgg caccatgaag tcagcggagg aagaacacta tggctatgca
     2461 tcctccaacg tcagccccgc cctgccgctc cccacggcgc actccaccct gccggccccg
     2521 tgccacaacc ttcagacctc cacaccgggc atcatcccgc cggcggatca cccctcgggg
     2581 tacggagcag ctttggacgg tgggcccgcg ggctacttcc tctcctccgg ccacaccagg
     2641 cctgatgggg cccctgccct ggagagtcct cgcatcgaga taacctcgtg cttgggcctg
     2701 taccacaaca ataaccagtt tttccacgat gtggaggtgg aagacgtcct ccctagctcc
     2761 aaacggtccc cctccacggc cacgctgagt ctgcccagcc tggaggccta cagagacccc
     2821 tcgtgcctga gcccggccag cagcctgtcc tcccggagct gcaactcaga ggcctcctcc
     2881 tacgagtcca actactcgta cccgtacgcg tccccccaga cgtcgccatg gcagtctccc
     2941 tgcgtgtctc ccaagaccac ggaccccgag gagggctttc cccgcgggct gggggcctgc
     3001 acactgctgg gttccccgcg gcactccccc tccacctcgc cccgcgccag cgtcactgag
     3061 gagagctggc tgggtgcccg ctcctccaga cccgcgtccc cttgcaacaa gaggaagtac
     3121 agcctcaacg gccggcagcc gccctactca ccccaccact cgcccacgcc gtccccgcac
     3181 ggctccccgc gggtcagcgt gaccgacgac tcgtggttgg gcaacaccac ccagtacacc
     3241 agctcggcca tcgtggccgc catcaacgcg ctgaccaccg acagcagcct ggacctggga
     3301 gatggcgtcc ctgtcaagtc ccgcaagacc accctggagc agccgccctc agtggcgctc
     3361 aaggtggagc ccgtcgggga ggacctgggc agccccccgc ccccggccga cttcgcgccc
     3421 gaagactact cctctttcca gcacatcagg aagggcggct tctgcgacca gtacctggcg
     3481 gtgccgcagc acccctacca gtgggcgaag cccaagcccc tgtcccctac gtcctacatg
     3541 agcccgaccc tgcccgccct ggactggcag ctgccgtccc actcaggccc gtatgagctt
     3601 cggattgagg tgcagcccaa gtcccaccac cgagcccact acgagacgga gggcagccgg
     3661 ggggccgtga aggcgtcggc cggaggacac cccatcgtgc agctgcatgg ctacttggag
     3721 aatgagccgc tgatgctgca gcttttcatt gggacggcgg acgaccgcct gctgcgcccg
     3781 cacgccttct accaggtgca ccgcatcaca gggaagaccg tgtccaccac cagccacgag
     3841 gccatcctct ccaacaccaa agtcctggag atcccactcc tgccggagaa cagcatgcga
     3901 gccgtcattg actgtgccgg aatcctgaaa ctcagaaact ccgacattga acttcggaaa
     3961 ggagagacgg acatcgggag gaagaacaca cgggtacggc tggtgttccg cgttcacgtc
     4021 ccgcaaccca gcggccgcac gctgtccctg caggtggcct ccaaccccat cgaatgctcc
     4081 cagcgctcag ctcaggagct gcctctggtg gagaagcaga gcacggacag ctatccggtc
     4141 gtgggcggga agaagatggt cctgtctggc cacaacttcc tgcaggactc caaggtcatt
     4201 ttcgtggaga aagccccaga tggccaccat gtctgggaga tggaagcgaa aactgaccgg
     4261 gacctgtgca agccgaattc tctggtggtt gagatcccgc catttcggaa tcagaggata
     4321 accagccccg ttcacgtcag tttctacgtc tgcaacggga agagaaagcg aagccagtac
     4381 cagcgtttca cctaccttcc cgccaacggt aacgccatct ttctaaccgt aagccgtgaa
     4441 catgagcgcg tggggtgctt tttcggatcc gactacaaag accatgacgg tgattataaa
     4501 gatcatgaca tcgactacaa ggatgacgat gacaagtagc ggccgcgaag gatctgcgat
     4561 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     4621 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     4681 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     4741 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt
     4801 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
     4861 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
     4921 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
     4981 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
     5041 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac
     5101 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc
     5161 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag
     5221 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg
     5281 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag
     5341 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac
     5401 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc
     5461 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag
     5521 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg
     5581 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac
     5641 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg
     5701 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac
     5761 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct
     5821 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt
     5881 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     5941 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     6001 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     6061 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     6121 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     6181 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     6241 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6301 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6361 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6421 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     6481 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     6541 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6601 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6661 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6721 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6781 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6841 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6901 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6961 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7021 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7081 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     7141 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7201 acgaagataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7261 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7321 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7381 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7441 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7501 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7561 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7621 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7681 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7741 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa
     7801 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7861 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7921 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7981 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8641 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8881 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8941 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9001 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9061 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9121 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9181 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9241 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9301 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9361 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9421 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9481 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9541 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9601 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9661 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9721 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9781 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9841 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9901 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9961 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    10021 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    10081 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    10141 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
    10201 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    10261 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    10321 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    10381 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
