LOCUS       V031676                11944 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031676
VERSION     V031676
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..6612
                     /label="NSD3(NM_023034)"
                     /note="NSD3(NM_023034)"
                     /gene="NSD3"
     CDS             6619..6663
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic
                     ribonuclease A"
                     /transl_table=1
                     /translation="KETAAAKFERQHMDS"
                     /label="S-Tag"
     CDS             6697..6720
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             6724..6747
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             6757..6870
                     /codon_start=1
                     /note="selected from a peptide library; binds streptavidin
                     with nanomolar affinity (Keefe et al., 2001)"
                     /product="streptavidin-binding peptide"
                     /transl_table=1
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
                     /label="SBP"
     promoter        6914..7125
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             7138..7406
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             7433..8032
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8039..8627
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8701..8934
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9006..9127
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9146..9281
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9319..9335)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9343..9359
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9367..9397)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9412..9433
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9721..10309)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10480..11340)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11341..11445)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11919..11935
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggatttc tctttctctt tcatgcaagg gatcatggga
     2341 aacacaattc agcaaccacc tcaactcatt gactccgcca acatccgtca ggaggatgcc
     2401 tttgataaca acagtgacat tgctgaagat ggtggccaga caccatatga agctactttg
     2461 cagcaaggct ttcagtaccc agctacaaca gaagatcttc ctccactcac aaatgggtat
     2521 ccatcatcaa tcagtgtgta tgaaactcaa accaaatacc agtcatataa tcagtatcct
     2581 aatgggtcag ccaatggctt tggtgcagtt agaaacttta gccccactga ctattatcat
     2641 tcagaaattc caaacacaag accacatgaa attctggaaa aaccttcccc tccacagcca
     2701 ccacctcctc cttcggtacc acaaactgtg attccaaaga agactggctc acctgaaatt
     2761 aaactaaaaa taaccaaaac tatccagaat ggcagggaat tgtttgagtc ttccctttgt
     2821 ggagaccttt taaatgaagt acaggcaagt gagcacacga aatcaaagca tgaaagcaga
     2881 aaagaaaaga ggaaaaaaag caacaagcat gactcatcaa gatctgaaga gcgcaagtca
     2941 cacaaaatcc ccaaattaga accagaggaa caaaatagac caaatgagag ggttgacact
     3001 gtatcagaaa aaccaaggga agaaccagta ctaaaagagg aagccccagt tcagccaata
     3061 ctatcttctg ttccaacaac ggaagtgtcc actggtgtta agtttcaggt tggcgatctt
     3121 gtgtggtcca aggtgggaac ctatccttgg tggccttgta tggtttcaag tgatccccag
     3181 cttgaggttc atactaaaat taacacaaga ggtgcccgag aatatcatgt ccagtttttt
     3241 agcaaccagc cagagagggc gtgggttcat gaaaaacggg tacgagagta taaaggtcat
     3301 aaacagtatg aagaattact ggctgaggca accaaacaag ccagcaatca ctctgagaaa
     3361 caaaagattc ggaaaccccg acctcagaga gaacgtgctc agtgggatat tggcattgcc
     3421 catgcagaga aagcattgaa aatgactcga gaagaaagaa tagaacagta tacttttatt
     3481 tacattgata aacagcctga agaggcttta tcccaagcaa aaaagagtgt tgcctccaaa
     3541 accgaagtta aaaaaacccg acgaccaaga tctgtgctga atactcagcc agaacagacc
     3601 aatgcagggg aggtggcctc ctcactctca agtactgaaa ttcggagaca tagccagagg
     3661 cggcacacaa gtgcggaaga ggaagagcca ccgcctgtta aaatagcctg gaaaactgcg
     3721 gcagcaagga aatccttacc agcttccatt acgatgcaca aagggagcct ggatttgcag
     3781 aagtgtaaca tgtctccagt tgtgaaaatt gaacaagtgt ttgctcttca gaatgctaca
     3841 ggggatggga aatttatcga tcaatttgtt tattcaacaa agggaattgg taacaaaaca
     3901 gaaataagtg tcagggggca agacaggctt ataatttcta caccaaacca gagaaatgaa
     3961 aagccaacgc agagtgtatc atctcctgaa gcaacatctg gttctacagg ctcagtagaa
     4021 aagaagcaac agagaagatc aattagaact cgttctgaat cagagaaatc cactgaggtt
     4081 gtgccaaaga agaagatcaa aaaggagcag gttgaaacag ttcctcaggc tacagtgaag
     4141 actggattac agaaaggtgc cagcgagatt tcagattcct gtaaacctct aaagaaaagg
     4201 agtcgcgcct caactgatgt agaaatgact agttcagcat acagagacac atctgactcc
     4261 gattctagag gactgagtga cctgcaggta ggctttggaa agcaagtaga tagcccttca
     4321 gctactgcag atgcagacgt ttctgatgtg cagtccatgg attcaagttt gtcgagaaga
     4381 ggcactggaa tgagtaagaa ggacactgta tgtcagattt gtgaaagctc tggtgactct
     4441 ctgattcctt gtgagggaga gtgctgcaaa cactttcacc tggagtgcct gggattggca
     4501 tcacttcctg atagcaagtt catctgcatg gaatgtaaaa ctgggcagca cccatgtttt
     4561 tcgtgtaaag tgtctggtaa agatgtgaag cgttgttctg ttggtgcttg tgggaaattt
     4621 tatcatgaag cctgtgtccg caaattcccc actgccatct ttgaatcaaa aggattccgc
     4681 tgtcctcagc actgctgctc tgcctgctct atggagaaag atatccacaa agcaagtaaa
     4741 ggccgcatga tgagatgttt aagatgtcca gttgcctatc actctggaga tgcttgcatt
     4801 gcggccggaa gcatgttagt atcctcctac attctcatct gtagtaatca ttccaaacgg
     4861 agcagtaatt cttctgctgt aaatgtaggc ttttgtttcg tttgtgccag agggctgata
     4921 gttcaggacc attcagaccc catgttcagt tcatatgcct ataagtccca ctacctactg
     4981 aatgaatcaa atcgtgctga gttgatgaaa ttacctatga ttccttcttc gtcagcttcc
     5041 aaaaagaaat gtgagaaagg tggaagattg ctctgctgtg aatcgtgccc agcttccttc
     5101 cacccggaat gcctaagcat agaaatgcca gaaggctgct ggaattgtaa tgactgtaaa
     5161 gctggcaaga aactacatta caagcagatt gtttgggtca aattgggaaa ttacagatgg
     5221 tggccagcag agatctgcaa ccccaggtct gtgccactga acatccaggg ccttaaacat
     5281 gacttggggg acttccctgt attcttcttt ggttctcatg actactactg ggtacaccag
     5341 ggcagagtgt tcccttatgt tgaaggagac aaaagctttg ctgaagggca gactagtatt
     5401 aacaagacct tcaaaaaggc actggaagaa gctgcaaaac gtttccagga attgaaagca
     5461 caaagagaaa gtaaagaagc cctagagatt gaaaaaaact caagaaaacc ccctccctac
     5521 aaacacatca aagctaacaa agtaatagga aaggtgcaga tccaggttgc tgacctgtca
     5581 gagattcccc gctgtaactg caagccagct gatgaaaacc cttgtggctt ggaatcggag
     5641 tgcctgaaca gaatgttgca gtatgaatgc cacccgcagg tgtgcccagc tggagatcgt
     5701 tgtcagaacc agtgctttac aaagagacta taccctgatg cagagatcat caaaacggag
     5761 cggagaggct ggggcctcag gaccaaaagg agcattaaga agggtgaatt tgtaaatgaa
     5821 tacgtcggtg aattaattga tgaagaagaa tgcagattgc gaatcaagcg agcccacgag
     5881 aacagtgtaa ctaattttta tatgttaact gttaccaagg accgtataat tgatgccggc
     5941 ccaaaaggaa attattctcg cttcatgaac cacagttgta atcccaactg tgaaacacaa
     6001 aagtggacag tgaatggaga tgttcgagtg ggactatttg ctctctgtga tattcctgca
     6061 gggatggagt taacatttaa ttataaccta gattgtctgg gcaacggcag aacggagtgc
     6121 cactgtggag cagataactg cagtggtttt ctaggagtgc ggccaaagtc ggcatgtgcg
     6181 tcaacaaatg aagagaaggc aaaaaatgct aagttaaaac agaagagacg aaagatcaaa
     6241 acagaaccaa agcagatgca tgaagattac tgttttcaat gtggagatgg tggagagctg
     6301 gtcatgtgtg acaaaaaaga ctgtcccaaa gcataccacc tcctatgcct taacctgact
     6361 cagccaccat atggaaagtg ggagtgtccg tggcatcagt gcgatgagtg cagcagtgca
     6421 gctgtttcct tctgtgaatt ctgtccacat tcattttgta aagatcatga aaagggggcc
     6481 ctggttccct ctgcactgga aggccgcctc tgctgctcgg aacatgaccc catggctcct
     6541 gtgtcaccag aatactggag caagataaaa tgtaaatggg aatcacaaga tcatggagaa
     6601 gaagtaaaag aaggatccaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac
     6661 agcggagccg gcgcaggagc cggcgctgac gcgcctgact acaaagacga tgacgacaag
     6721 ggagattaca aggatgacga tgacaaaggc gcgtcgatgg acgagaagac caccggctgg
     6781 cggggcggcc acgtggtgga gggcctggcc ggcgagctgg agcagctgcg ggccaggctg
     6841 gagcaccacc ctcagggcca gcgggagccc tagcggccgc gaaggatctg cgatcgctcc
     6901 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     6961 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     7021 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     7081 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     7141 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     7201 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     7261 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     7321 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     7381 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga
     7441 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     7501 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     7561 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     7621 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     7681 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     7741 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     7801 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     7861 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     7921 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     7981 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa
     8041 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     8101 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     8161 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     8221 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     8281 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     8341 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     8401 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     8461 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     8521 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     8581 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa
     8641 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8701 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc
     8761 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8821 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8881 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8941 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     9001 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9061 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9121 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc
     9181 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9241 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg
     9301 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9361 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9421 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9481 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9541 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9601 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9661 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9721 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9781 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9841 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9901 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9961 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10021 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10081 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10141 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    10201 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10261 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10321 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10381 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10441 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10501 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10561 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10621 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10681 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10741 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10801 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10861 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10921 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10981 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    11041 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    11101 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11161 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11221 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11281 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11341 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11401 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11461 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    11521 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    11581 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    11641 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    11701 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    11761 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    11821 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    11881 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    11941 gctg
//
