LOCUS       V040853                10519 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040853
VERSION     V040853
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5373
                     /label="Nat10(NM_001399512)"
                     /note="Nat10(NM_001399512)"
                     /gene="Nat10"
     CDS             5380..5445
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5489..5700
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5713..5981
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6008..6607
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6614..7202
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7276..7509
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7581..7702
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7721..7856
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7894..7910)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7918..7934
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7942..7972)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7987..8008
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8296..8884)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9055..9915)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9916..10020)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10494..10510
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgcatcgg aagaaggtgg ataaccgaat tcgaattctc
     2341 attgagaatg gcgtatctga gcggcagagg tctctttttg ttgtagttgg agatcgagga
     2401 aaagatcagg tggttattct tcatcatatg ttgtccaaag caactgtgaa ggctcggccc
     2461 tctgtcctgt ggtgttacaa gaaagagctg ggatttagca gtcatcggaa gaagagaatg
     2521 cggcagctac agaagaagat aaagagtggg accttgaaca taaagcaaga tgaccccttt
     2581 gagctttttg tagcagccac aaacattcgc tactgctact acaatgaaac ccacaagatt
     2641 ctgggcaaca cttttggcat gtgtgtcctc caggactttg aagcattaac tccaaacttg
     2701 ttggccagaa ctgtagaaac agtagaaggt ggtggattgg tggtcatcct cttacggacc
     2761 atgaactcgc ttaagcagct gtacacaatg actatggatg tgcactccag gtacaggacc
     2821 gaggcccatc aggacgtggt gggaaggttt aacgagaggt ttattctctc tctggcatct
     2881 tgtaagaagt gtctggtcat tgatgaccaa ctcaatatcc tgcccatctc ctcccatgtg
     2941 gccagcattg aagccttacc tcctcaggcc cccgatgaga atctcagtcc tgctgctctg
     3001 gagctgcagg agttgaaaga gagcttgcag gacactcagc ccgtgggtgt tttggtggac
     3061 tgctgcaaga ccctggacca ggccaaagct gtcttgaaat tcattgaggg gatctcggag
     3121 aagaccctaa ggagtactgt tgccctcacc gctgcccgag gacggggcaa gtctgcggcc
     3181 ctgggcctgg ctattgctgg ggcagtggca ttcggatatt ccaatatttt tgtcacctcc
     3241 ccaagcccag ataacctcca caccctgttt gaatttgtat ttaaggggtt tgatgctctg
     3301 cagtatcagg agcacctgga ctacgagatt gtacagtcgc tgaaccccga gtttaataaa
     3361 gcggtgatca gggtaaatgt gttccgagag cacaggcaga ctatccagta catccatcct
     3421 gcagatgctg tgaaactggg ccaggctgag ctggttgtga tagatgaagc tgccgctatt
     3481 cccctccccc tggtgaagag cttgcttggg ccctacctgg ttttcatggc atctactatc
     3541 aatggctacg agggcactgg ccggtcactt tccctcaaac tcatccagca actccgtcag
     3601 cagagtgccc agagccaggt cagcaccact gctgagaaca agaccacgac gacagccaga
     3661 ttggcctcag ctcggacatt gcatgaggtt tccctccaag agtcaatccg gtacgcccct
     3721 ggggatgcag tggagaagtg gctgaatgac ctgctgtgcc tggattgcct caacatcacc
     3781 cgcatcgttt ccggctgccc cttgcctgag gcctgtgagc tctactatgt taacagagat
     3841 accctctttt gctaccacaa ggcttctgag gtttttctgc agaggctcat ggctctctat
     3901 gtggcttcgc attacaagaa ctctcccaac gatctgcaga tgctctcgga tgcacctgct
     3961 caccacctct tctgccttct gccacctgtg ccccctaccc agaatgccct gcccgaagtg
     4021 cttgcagttg tccaggtgtg ccttgaagga gagctttctc gtcagtccat tttgaatagc
     4081 ctgtctagag gcaaaaaggc ttctggggac ctgcttccat ggactgtgtc agaacagttt
     4141 caggatccag actttggagg cctttctggt ggacgggttg ttcgaattgc tgtacatcca
     4201 gattaccaag ggatgggcta cggcagccga gcactgcagc tgctgcagat gtactacgaa
     4261 ggcaggttcc cgtgtctgga ggaaaaggtc ttggagacac cacaagaaat ccacaccgtg
     4321 agcagtgagg ccgtcagctt gttggaagaa gttatcactc cccggaagga tctgcctccc
     4381 ttactcctca agttgaatga gaggcctgca gagcgcctag attatctggg ggtttcctat
     4441 gggctgaccc ccaggctcct caagttctgg aaacgagctg gatttgttcc tgtctatttg
     4501 agacagaccc caaatgacct gactggagag cactcctgca ttatgctgaa gacactggct
     4561 gatgaggatg aggcggagcg gggagcttgg ctggcagcat tttggaccga tttccgaaga
     4621 cggttcctgg ccctgctgtc ttaccagttc agcaccttct ctcctgccct gtctctgaac
     4681 atcattcaga acaggaacat agcgaagtca gccccaccag tcctaggccg ggagcatctg
     4741 gaggccctct tcctccccta tgacctgaag cggctggaga tgtactctcg gaacatggtc
     4801 gattatcacc tcatcatgga ccttatccca gccatttccc gcctgtactt cctcaaccag
     4861 ctaggggacc tggcattgtc agccgcccag tcggctcttc tcctgggaat gggcctacag
     4921 cacaagtccg tggatcagct ggaaaaggag atcgagctac cctcaggcca gttgatgggg
     4981 cttttcaacc ggatcatccg caaagtcgtg aagctgttca atgatgtgca ggaaaaggct
     5041 attgaggagc agatggtggc agtgaaggat gtggtcatgg agcctacgat aaagacgctg
     5101 agtgatgacc tggatgaagc agcaaaggaa ttccaggaga aacacaagaa ggacgtgggg
     5161 aagctgaagg acatggacct ctctcaatat ataatccgtg gcgatgatga agagtggaat
     5221 gaagttttga gcaaagcagg gcagaatgcc tccattgtta gtttgaaaag tgacaagaaa
     5281 aggaaactgg aaacaaaaca agaacccaaa cagagcaaga agttgaagaa gagcggtaac
     5341 aacagaaagg acacgaaact gaagagaaag aagggatccg actacaaaga ccatgacggt
     5401 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     5461 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     5521 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     5581 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     5641 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     5701 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5761 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5821 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5881 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5941 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     6001 acgctagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     6061 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6121 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6181 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6241 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     6301 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6361 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6421 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     6481 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6541 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     6601 tgcctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6661 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6721 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6781 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6841 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6901 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6961 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     7021 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7081 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7141 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7201 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7261 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg
     7321 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7381 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7441 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7501 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7561 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     7621 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7681 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7741 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7801 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga
     7861 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7921 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7981 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8041 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8101 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8161 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8221 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8281 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8341 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8401 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8461 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8521 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8581 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8641 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8701 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8761 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8821 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8881 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8941 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9001 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9061 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9121 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9181 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9241 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9301 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9361 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9421 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9481 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9541 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9601 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9661 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9721 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9781 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9841 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9901 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9961 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10021 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10081 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    10141 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10201 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    10261 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    10321 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
    10381 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    10441 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
    10501 gacggccagt gccaagctg
//
